龚洁敏 金志 孔德骞 郑学勤 金建华 刘芝华 陈章良
1994, 15(1): 65-71.
Total RNA was isolated from banana fruit (Musa nana Lour). The first strand of cDNA was synthesized with Oligo dT as a primer. The calmodulin gene of banana was obtained by PCR techniques vith primers synthesized according to the two oligo-polynucleotide extremities of the DNA sequence of barley calmodulin gene and cloned into BluescriptSK" vector. A full length of cDNA encoding calmodulin gene was sequenced and analysed. Its nucleotide sequences share 81.9%-95.3% sequence identities as compared with calmodulin genes of alfafa, rice, barley, potato, carrot, maize ?and pea. It was cloned into protein expression vector pTrcHisB and obtained high levels of expression in E. coli.