1 材料与方法
1.1 材料
1.2 方法
表1 试验设计Tab.1 Experimental design |
| 处理 Treatment | 氮肥 Amount of nitrogen fertilizer/(g·cluster-1) | 保水剂 Amount of water retaining agent/(g·cluster-1) |
|---|---|---|
| CK | 0 | 0 |
| A | 150 | 0 |
| B | 300 | 0 |
| C | 450 | 0 |
| D | 150 | 180 |
| E | 300 | 180 |
| F | 450 | 180 |
1.3 数据处理
2 结果与分析
2.1 保水剂与氮肥对鼓节竹叶片光合特征参数影响的差异分析
表2 保水剂与氮肥对鼓节竹叶片光合特征参数影响的差异分析Tab. 2 Difference analysis of the effects of water retaining agent and nitrogen fertilizer on photosynthetic characteristic parameters of B. tuldoides |
| 变量 Variable | 平方和 Sum of squares | 自由度 df | 均方 Mean square | F值 F-value | 显著性水平 Significance |
|---|---|---|---|---|---|
| Chl(a+b) | 0.92 | 6 | 0.15 | 176.19 | 0 |
| Pn | 87.40 | 6 | 14.57 | 77.41 | 0 |
| Gs | 0.01 | 6 | 0.002 | 59.62 | 0 |
| Ci | 728 759.41 | 6 | 121 459.90 | 43.83 | 0 |
| Tr | 8.01 | 6 | 1.33 | 10.70 | 0 |
| WUE | 2.46 | 6 | 0.41 | 19.98 | 0 |
| Fo | 3 665.33 | 6 | 610.89 | 7.56 | 0.001 |
| Fv/Fm | 0.22 | 6 | 0.04 | 79.68 | 0 |
| Fv/Fo | 4.24 | 6 | 0.71 | 31.29 | 0 |
| ΦPSⅡ | 0.08 | 6 | 0.01 | 48.21 | 0 |
| ETR | 273.02 | 6 | 45.50 | 63.87 | 0 |
| qP | 0.02 | 6 | 0.00 | 39.78 | 0 |
| qN | 0.05 | 6 | 0.01 | 39.27 | 0 |
2.2 保水剂与氮肥对鼓节竹叶片叶绿素含量的影响
表3 保水剂与氮肥对鼓节竹叶片叶绿素含量的影响Tab. 3 Effects of water-retaining agent and nitrogen fertilizer on chlorophyll content of B. tuldoides |
| 处理Treatments | Chl(a+b)/(mg·g-1) |
|---|---|
| CK | 2.02±0.02f |
| A | 2.23±0.02e |
| B | 2.46±0.03c |
| C | 2.68±0.02a |
| D | 2.22±0.04e |
| E | 2.41±0.03d |
| F | 2.54±0.02b |
Note: Different lowercase letters in the same column indicate significant difference at 0.05 level. | |
注:同列不同小写字母表示处理间差异显著(P<0.05)。 |
2.3 保水剂与氮肥对鼓节竹叶片气体交换参数的影响
表4 保水剂与氮肥对鼓节竹叶片气体交换参数的影响Tab. 4 Effects of water-retaining agent and nitrogen fertilizer on gas exchange parameters in leaves of B. tuldoides |
| 处理 Treatments | Pn /(μmol·m-2∙s-1) | Gs /(mol∙m-2∙s-1) | Ci /(μmol·m-2∙s-1) | Tr /(mmol∙m-2∙s-1) | WUE /(μmol∙m-2∙s-1) |
|---|---|---|---|---|---|
| CK | 2.69±0.07e | 0.035±0.000 09d | 298.30±0.46c | 2.38±0.11b | 1.12±0.11d |
| A | 3.59±0.07d | 0.039±0.000 03d | 334.89±0.42dc | 2.44±0.17b | 1.48±0.10c |
| B | 4.18±0.08d | 0.037±0.000 04d | 405.49±0.42d | 2.90±0.13b | 1.44±0.10c |
| C | 6.72±0.07b | 0.075±0.000 05b | 563.66±0.39c | 3.67±0.14a | 1.84±0.09b |
| D | 5.67±0.07c | 0.052±0.000 06c | 568.06±0.48c | 3.03±0.12b | 1.86±0.12b |
| E | 7.95±0.07a | 0.090±0.000 07a | 841.58±0.44b | 3.69±0.12a | 2.15±0.15a |
| F | 8.41±0.06a | 0.100±0.000 08a | 841.58±0.43a | 4.13±0.16a | 2.04±0.18b |
Note: Different lowercase letters in the same column indicate significant difference at 0.05 level. | |
注:同列不同小写字母表示处理间差异显著(P<0.05)。 |
2.4 保水剂与氮肥对鼓节竹叶片叶绿素荧光参数的影响
表5 保水剂与氮肥对鼓节叶片叶绿素荧光参数的影响Tab. 5 Effects of water-retaining agent and nitrogen fertilizer on chlorophyll fluorescence parameters of B. tuldoides |
| 处理 Treatment | Fo | Fv/Fm | Fv/Fo | ΦPSⅡ | ETR | qP | qN |
|---|---|---|---|---|---|---|---|
| CK | 196.33±4.98cd | 0.68±0.02d | 2.13±0.13c | 0.57±0.04d | 27.77±0.83e | 0.84±0.01d | 0.24±0.01d |
| A | 224.67±5.36a | 0.76±0.01cd | 2.26±0.11c | 0.64±0.02c | 32.60±0.06d | 0.84±0.01c | 0.24±0.01cd |
| B | 212.67±5.28abc | 0.75±0.02c | 3.04±0.04b | 0.69±0.03b | 33.76±0.73cd | 0.85±0.02bc | 0.26±0.01c |
| C | 217.67±7.06ab | 0.83±0.02b | 2.95±0.03b | 0.69±0.01b | 34.90±0.36c | 0.87±0.01b | 0.30±0.02b |
| D | 203.33±2.73d | 0.73±0.01c | 3.19±0.07ab | 0.67±0.02b | 36.70±0.06b | 0.85±0.01bc | 0.35±0.01a |
| E | 181.66±4.48bc | 0.95±0.01a | 3.40±0.07a | 0.78±0.02a | 38.70±0.38a | 0.91±0.01a | 0.34±0.01a |
| F | 205.67±6.84bc | 0.95±0.02a | 3.14±0.05ab | 0.75±0.03a | 38.90±0.62a | 0.93±0.01a | 0.35±0.01a |
Note: Different lowercase letters indicate significant difference at 0.05 level. | |
注:同列不同小写字母表示处理间差异显著(P<0.05)。 |
2.5 保水剂与氮肥对鼓节竹叶片光合特征参数的相关性分析
表6 保水剂与氮肥对鼓节竹叶片光合特征参数的相关性分析Tab. 6 Correlation analysis of water-retaining agent and nitrogen fertilizer on photosynthetic characteristic parameters of leaves of B. tuldoides |
| 指标 Index | Chl(a+b) | Pn | Gs | Ci | Tr | WUE | Fo | Fv/Fm | Fv/Fo | ΦPSⅡ | ETR | qP | qN |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chl(a+b) | 1.000 | ||||||||||||
| Pn | 0.682** | 1.000 | |||||||||||
| Gs | 0.631** | 0.957** | 1.000 | ||||||||||
| Ci | 0.586** | 0.952** | 0.930** | 1.000 | |||||||||
| Tr | 0.708** | 0.919** | 0.904** | 0.867** | 1.000 | ||||||||
| WUE | -0.708** | -0.919** | -0.904** | -0.867** | -1.000** | 1.000 | |||||||
| Fo | 0.185 | -0.268 | -0.309 | -0.281 | -0.133 | -0.133 | 1.000 | ||||||
| Fv/Fm | 0.652** | 0.910** | 0.938** | 0.875** | 0.835** | -0.835** | -0.380* | 1.000 | |||||
| Fv/Fo | 0.602** | 0.766** | 0.638** | 0.754** | 0.666** | -0.666** | -0.399* | 0.692** | 1.000 | ||||
| ΦPSⅡ | 0.710** | 0.863** | 0.817** | 0.835** | 0.746** | -0.746** | -0.286 | 0.900** | 0.841** | 1.000 | |||
| ETR | 0.607** | 0.884** | 0.781** | 0.889** | 0.737** | -0.737** | -0.195 | 0.795** | 0.846** | 0.901** | 1.000 | ||
| qP | 0.574** | 0.895** | 0.919** | 0.903** | 0.807** | -0.807** | -0.339 | 0.939** | 0.638** | 0.863** | 0.797** | 1.000 | |
| qN | 0.406* | 0.857** | 0.756** | 0.896** | 0.711** | -0.711** | -0.336 | 0.681** | 0.792** | 0.731** | 0.863** | 0.721** | 1.000 |
Note: ** indicates extremly significant correlation at 0.01 level (1-tailed); * indicates significant correlation at 0.05 level (1-tailed). | |
注:**表示在0.01水平(单侧)上极显著相关;*表示在0.05水平(单侧)上显著相关。 |
2.6 保水剂与氮肥处理的主成分分析
表7 保水剂与氮肥处理的主成分分析Tab. 7 Principal component analysis of water retaining agent and nitrogen fertilizer treatment |
| 处理Treatments | 得分Scores | 排名Rankings |
|---|---|---|
| CK | -12.57 | 7 |
| A | -8.15 | 6 |
| B | -3.89 | 5 |
| C | 3.15 | 3 |
| D | 0.12 | 4 |
| E | 10.02 | 2 |
| F | 11.32 | 1 |
