In order to find and develop safe and effective botanical insecticides, to enrich the sources of botanical pesticides and fill the gaps left by prohibitions and restrictions on the use of chemical pesticides, sixteen kinds of Chinese herbal medicines were extracted by methanol, and the insecticidal activity against adult Musca domestica was tested by the method of unlimited intake, and that against the fourth instar larvae of Aedes aegypti was tested by the larval immersion method. The results showed that after 48 h treatment at 10 mg/mL, the corrected mortality rates of Asarum heterotropoides F. Schmidt var. mandshuricum (Maxim.) Kitag, Piper wallichii (Miq.) Hand-Mazz, Aristolochia kaempferi Willd. [A.mollis Dunn], Asarum himalaicum Hook. f. et Thoms. ex Klotzsch was 100%, 100%, 100% and 98.33%, respectively, and the LC50 value separately was 0.1483, 0.1544, 3.5277 and 2.4292 mg/mL, while that for Derris trifoliata Lour was 0.9080 mg/mL. Against the 4 th instar larvae of A. aegypti at 0.5 mg/mL for 48 h, the corrected mortality rate of A. heterotropoides, P. wallichii, A. kaempferi, A. himalaicum, and Magnolia officinalis Rehd. et Wils. was 100%, 100%, 100%, 100% and 100%, separately, and the LC50 value was 0.0046, 0.0094, 0.0570, 0.0658 and 0.0504 mg/mL, respectively, while that for D. trifoliata Lour was 0.1194 mg/mL. A. heterotropoides, P. wallichii, A. kaempferi, A. himalaicum showed excellent insecticidal activity on the two tested insects, better than or close to the activity of the control D. trifoliata.