In order to lay the foundation for scientific evaluation of fruit quality of wampee germplasms, 11 fruit quality indices including single fruit weight, fruit length, fruit diameter, fruit shape index, edible rate, total soluble solid, vitamin C, protein, soluble sugar, total acid, sugar-acid ratio for the mature fruits of 23 wampee germplasms resources were measured and analyzed using principal component analysis and cluster analysis. Lastly, the representative quality evaluation indexes were selected from indices, 23 wampee germplasms resources were clustered according to the selected indexes. The results showed that the variation of single fruit weight, vitamin C, protein, soluble sugar, total acid and sugar-acid ratio were above 20%, while the variation of fruit diameter and fruit shape index were below 10%. Among them, the variation of sugar-acid ratio of wampee germplasms was the most significant, which was as high as 108.57%, while the variation of fruit shape index was the most non-significant, only 6.98%. This indicated that the content of nutrients varied greatly among different wampee germplasm, with abundant genetic information and diversity, and the appearance and size of them were genetically stable, and had less space for improvement. There were significant or extremely significant correlations among the quality indexes of the germplasm. After standardizing the data, the principal component analysis was carried out, and four representative principal components were extracted, of whom cumulative proportion reached 85.41%. According to the score of the 1st to 4th principal component factor and the comprehensive score of the quality indexes, the first and second principal components played a key role in the comprehensive evaluation of wampee quality, the highest score was A3, and the lowest score was G1. Meanwhile, the six indexes which has great influence and relatively independent on the quality of wampee germplasm resources were single fruit weight, fruit shape index, edible rate, soluble sugar, total acid and sugar-acid ratio. The systematic cluster analysis of fruit quality in wampee by the 6 selected and representative indexes which were standardized showed that the fruit quality from the germplasm resources could be clustered into 4 groups according to Euclidean distance of 15. The 1st group had A3 and Y-2, which were the largest, and had a high edible rate and sugar-acid ratio, with a sweet flavor and excellent quality. The 2nd group had X1, Y-1, G2 and B1, the fruits were larger and diverse in shapes, with high edible rates, but relatively low in soluble sugar and sugar acids, and inferior quality. The 3rd group included 300-1, J2, 300-2, 9-3, H1, A1, Z1, A2 and N-3, which had low fruit size, high edible rate, moderate sour and sweet. The 4th group included A5, N-1, H2, A6, T1, G1, J1, J3, which had small fruit, low edible rate, low soluble sugar, high total acid, low sugar-acid ratio and poor taste.