In order to select the best fertilization mode for passion fruit, five different fertilization treatments were set up in this study: no fertilization (CK), nitrogen, phosphorus and potassium fertilizer (NPK), organic fertilizer (M), nitrogen, phosphorus and potassium combined with organic fertilizer (MNPK), high amount of nitrogen, phosphorus and potassium and high amount of organic fertilizer (2MNPK). After the analysis and comparison of the difference of yield and nutritional components of passion fruit, the principal component analysis and comprehensive evaluation were done by the multivariate statistical analysis method (PCA). The results showed that compared with CK, the growth of passion fruit treated with NPK, MNPK and 2MNPK was the fastest, especially after 66 days of planting. NPK, M, MNPK and 2MNPK increased the yield and quality, MNPK and 2MNPK treatments was the most obvious. Compared with CK, the yield of passion fruit treated with NPK, M, MNPK and 2MNPK increased by 112.86%, 74.40%, 191.00% and 244.30%, respectively, and the content of vitamins, mineral elements, sugars and amino acids increased, while the contents of total acids decreased. The content of total amino acids, essential amino acids and flavor amino acids increased by 82.40%, 76.11% and 76.89%, respectively, and the total acid content decreased by 22.61% in 2MNPK. Based on the multivariate statistical analysis of 36 indexes, three principal component factors were extracted, and the contribution rate of cumulative variance was 97.925%. According to the variance contribution rate of the three principal components, the comprehensive evaluation model was established to calculate the comprehensive total scores of different fertilization treatments, and the order was 2MNPK (123.04)>MNPK (39.06)>M (-12.01)>NPK (-45.36)>CK (-104.73). It shows that among the five fertilization treatments, 2MNPK is the best fertilization mode in the process of passion fruit planting. This study could lay a theoretical foundation for the large-scale popularization and application of passion fruit.