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  • Germplasm Resources, Genetics & Breeding
    ZHOU Yan, LI Dongmei, WU Kunlin, NING Zulin
    Chinese Journal of Tropical Crops. 2023, 44(8): 1588-1595. https://doi.org/10.3969/j.issn.1000-2561.2023.08.008

    Ardisia gigantifolia Stapf is native to Hekou county, Yunnan province, with few wild individuals, and is listed as a critically endangered species. It has important protection value and good prospect of garden development and application. The stem segments of aseptic seedlings of A. gigantifolia were used as the explants to establish a propagation in vitro. The results showed that the optimal medium for callus induction was WPM+0.50 mg/L TDZ+0.10 mg/L NAA, and the induction rate of callus was 96.67%. The optional medium for callus proliferation was WPM+0.50 mg/L TDZ and the multiplication coefficient was 4.42. The optimal medium for callus differentiation was WPM+2.00 mg/L 6-BA+0.20 mg/L NAA, and the induction rate reached 100%. The optional medium for adventitious bud proliferation culture was WPM+4.00 mg/L 6-BA, the multiplication coefficient was 3.90, and the cluster buds grew well. The optional rooting medium was WPM+10% coconut water+0.20 mg/L NAA, the rooting rate reached 100%, the root system developed well and the test-tube plantlets grew vigorously. Plantlets were transplanted into the mixture substrate with the volume ratio of perlite and peat soil of 1∶3, the survival rate was 96.67% after 60 days and plants grew well. This study can provide technical support for the large-scale seedling production of A. gigantifolia, as well as theoretical basis and technical support for the protection, seedling breeding, and molecular biology research of this species.

  • Plant Cultivation, Physiology & Biochemistry
    LI Youli, JI Xunzhi, QIN Xiaowei, YU Huan, ZHANG Ang, ZONG Ying, HE Shuzhen
    Chinese Journal of Tropical Crops. 2023, 44(6): 1161-1169. https://doi.org/10.3969/j.issn.1000-2561.2023.06.010

    In this experiment, Pandanus amaryllifolius Roxb. plantlets in vitro were used as detected material. In order to investigate the most suitable fertilization formula for increasing aroma of P. amaryllifolius Roxb. plantlets in vitro. And the volatile components of P. amaryllifolius Roxb. plantlets in vitro were separated and identified by gas chromatography-mass spectrometry (GC-MS), and the results were analyzed by principal component analysis (PCA) and cluster heatmap analysis. The results showed that the volatile components were similar in seven different treatments, but their volatile contents were different greatly (P<0.05). A total of 21 volatile components were detected, including alcohols, pyrroles, esters, ketones, furans, furans, acids, olefins and phenols, and squalene, phytol. Among them 2-acetyl-1-pyrroline (2AP), neophytadiene, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran were the main volatile components. The average content of the characteristic aroma component 2AP were (48.07±13.14)μg/g of P. amaryllifolius Roxb. plantlets in vitro, and the treatment 6 (zinc+fertilizer combinations) was hightest to 72.03 μg/g; however the treatment 1 (IBA+zinc+fertilizer combinations) was the lowest to 31.96 μg/g, and the treatment 6 could significantly increase the content of the characteristic aroma component 2AP. The results of principal component analysis showed significant difference between the fertilizer combinations treatment (ammonium bicarbonate + calcium superphosphate+potassium chloride) and the water treatment, and the main volatile components of the treatment 2 and the treatment 6 were phytol, 2AP, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran, neophytadiene; and the main volatile components of the treatment 1 and the treatment 5 were ethyl oleate-based esters, 2,4-di-tert-butylphenol-based phenols, acetol-based ketones. The volatile components were mainly squalene in the treatment 3, treatment 4 and treatment 7. Cluster analysis showed that higher volatile component in the treatment 6 (zinc+fertilizer combinations), and the volatile component were significantly lower in the treatment 1 (IBA+zinc+fertilizer combinations). There were no obviously differences in other treated groups. The conclusion indicated that zinc sulfate immersion and ammonium bicarbonate+calcium superphosphate+potassium chloride fertilizer can improve aroma than other groups.