An early-maturing, high-yield, high-starch, wide-adaptability cassava cultivar was bred in Guangxi for fresh consumption or further processing. A newly bred F₁ progeny of crossing between the high-yield, XX048 as the female parent and the high-starch NZ199 as the male parent was generated by artificial pollination and induction through prediction of graft breeding. After seedling selection, single row and a series of primary, intermediate, and advanced tests as well as regional and production trials, Guire No. 13 (GR13) was selected. As showed in the several years trial, GR13 delivered an average tuber yield of 47.06 t/hm², starch content was 32.0%, which was 22.93% and 21.48, 3.74 and 5.30 percentage point higher than that of NZ199 and SC205; Dry matters content of 45.2%, which was 4.7 and 7.5 percentage point higher than that of NZ199 and SC205, and HCN content was 25.7 mg/kg, lower than that of NZ199 and SC205; a rich content of nutrients (based on FW), such as 0.73% on crude fiber, 42.87 mg/hg on vitamin C, 0.75 g/hg on protein, 3% on total sugar, 1.1% on soluble sugar. The early-maturing (over 27.0% on starch content after planted 180 days), high and stable yield GR13 with wide-adaptability was resistent to drought, cold and mites, and its leaves did not fall off easily. It could be adequately cultivated in current cassava-producing areas in Guangxi, Jiangxi, and Yunnan provinces for either fresh consumption, further processing, or as a raw material for manufacturing starch products.

The proteins on rubber particles of Hevea brasiliensis play key roles in a series of reactions of rubber biosynthesis. They directly determine the number and size of rubber molecules, thus affecting the yield and quality of natural rubber. Small rubber particle protein (SRPP) is the second highest abundance protein in rubber particles following after the rubber elongation factor (REF), which is closely related to rubber particle development and rubber biosynthesis. At present, it is known that there are many SRPP family proteins on rubber particles, but the functions of most SRPP family proteins have not been identified. SRPP may function by interacting with other rubber particle proteins. To screen the interaction protein of HbSRPP7, a normalized H. brasiliensis latex cDNA library was constructed based on the membrane yeast two-hybrid (MYTH) system. The capacity of the cDNA library was 1.5×10⁷ CFU, the average library recombination rate was about 100%, and the average amplification sizes of insert fragments in the cDNA library were above 1500 bp. The pBT3STE-SRPP7 and pBT3SUC-SRPP7 bait vectors were constructed and confirmed that they could be correctly expressed in the NMY32 yeast strain without self-activating activity. The latex MYTH cDNA library was screened using the bait plasmid pBT3STE-SRPP7, and 21 candidate proteins of HbSRPP7 were obtained, including three REF family proteins (HbREF1, HbREF3, and HbREF8), two SRPP family proteins (HbSRPP1 and HbSRPP2), two reactive oxygen species scavenging-related proteins (thioredoxin H-type-like and L-ascorbate peroxidase 2), and five stress-related proteins (high mobility group B protein 2-like, RPM1-interacting protein 4-like, stress-related protein-like, salt stress-induced hydrophobic peptide ESI3-like and F-box/kelch-repeat protein). It is shown that HbSRPP7 may participate in rubber biosynthesis through interaction with the rubber particle proteins related to rubber biosynthesis. In addition, HbSRPP7 may also participate in the regulation of rubber biosynthesis on rubber particles by interacting with biotic and abiotic stress-related proteins and responding to biotic and abiotic stress signals in laticiferous cells of H. brasiliensis. The research results are helpful to understand the function of SRPP family proteins and lay a foundation for revealing the composition of protein complexes involved in rubber biosynthesis on rubber particles and elucidating the molecular mechanism of rubber biosynthesis and its regulation.