Chinese Journal of Tropical Crops
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    Effects of Phenolic Substances and Related Enzyme Activities in the Process of Tissue Culture Browning of Artocarpus heterophyllus Stem Tips of ‘Haida 2’
    DING Yan, GUO Jiahui, FAN Ziyun, XIANG Xingxing, GAN Yulu, MIN Tianyu, YANG Ting, FENG Feng, YE Chunhai
    Chinese Journal of Tropical Crops    2023, 44 (3): 562-568.   DOI: 10.3969/j.issn.1000-2561.2023.03.013
    Abstract1581)   HTML19)    PDF(pc) (1317KB)(63)       Save

    Artocarpus heterophyllus is popular for its sweet fruit and has a very promising market, but conventional seed propagation makes it difficult to maintain its excellent traits and the grafting method has a very low survival rate. The technique of tissue culture and rapid propagation has been widely used in the propagation of many rare and valuable plants, but there are no reports of its application in the production of A. heterophyllus. In the process of tissue culture, it was found that the stem tip of A. heterophyllus was severely browned, which led to the difficulty of establishing asexual reproduction system. In order to effectively investigate the measures to overcome the browning, this experiment took the stem tips of A. heterophyllus ‘Haida 2’ as the explants and inoculated them into different concentrations of PVP medium after low-temperature pretreatment at different lengths for primary culture, the browning rate and germination rate were calculated after 15 d, and PPO activity, total phenol and total flavonoid content were determined to explore the inhibition of different concentrations of PVP on browning in stem the tip tissue culture of A. heterophyllus after low-temperature pretreatment. The results showed that the browning rate of A. heterophyllus explants was the lowest and the germination rate was the highest after 12-24 h low-temperature treatment of stem tip and then in 1/2MS medium supplemented with 2.0 g/L PVP. The experimental results showed that this method was beneficial to the proliferation of A. heterophyllus explants. The purpose of this experiment is to promote the establishment of technique of tissue culture and rapid propagation for A. heterophyllus, with a view to providing a theoretical basis for the construction of the A. heterophyllus seedling breeding technology system and its industrial production demonstration.

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    Establishment of Regeneration System of Stem Callus for Ardisia gigantifolia, a Rare and Endangered Species
    ZHOU Yan, LI Dongmei, WU Kunlin, NING Zulin
    Chinese Journal of Tropical Crops    2023, 44 (8): 1588-1595.   DOI: 10.3969/j.issn.1000-2561.2023.08.008
    Abstract1559)   HTML11)    PDF(pc) (4043KB)(56)       Save

    Ardisia gigantifolia Stapf is native to Hekou county, Yunnan province, with few wild individuals, and is listed as a critically endangered species. It has important protection value and good prospect of garden development and application. The stem segments of aseptic seedlings of A. gigantifolia were used as the explants to establish a propagation in vitro. The results showed that the optimal medium for callus induction was WPM+0.50 mg/L TDZ+0.10 mg/L NAA, and the induction rate of callus was 96.67%. The optional medium for callus proliferation was WPM+0.50 mg/L TDZ and the multiplication coefficient was 4.42. The optimal medium for callus differentiation was WPM+2.00 mg/L 6-BA+0.20 mg/L NAA, and the induction rate reached 100%. The optional medium for adventitious bud proliferation culture was WPM+4.00 mg/L 6-BA, the multiplication coefficient was 3.90, and the cluster buds grew well. The optional rooting medium was WPM+10% coconut water+0.20 mg/L NAA, the rooting rate reached 100%, the root system developed well and the test-tube plantlets grew vigorously. Plantlets were transplanted into the mixture substrate with the volume ratio of perlite and peat soil of 1∶3, the survival rate was 96.67% after 60 days and plants grew well. This study can provide technical support for the large-scale seedling production of A. gigantifolia, as well as theoretical basis and technical support for the protection, seedling breeding, and molecular biology research of this species.

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