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  • Germplasm Resources, Genetics & Breeding
    ZHOU Yan, LI Dongmei, WU Kunlin, NING Zulin
    Chinese Journal of Tropical Crops. 2023, 44(8): 1588-1595. https://doi.org/10.3969/j.issn.1000-2561.2023.08.008

    Ardisia gigantifolia Stapf is native to Hekou county, Yunnan province, with few wild individuals, and is listed as a critically endangered species. It has important protection value and good prospect of garden development and application. The stem segments of aseptic seedlings of A. gigantifolia were used as the explants to establish a propagation in vitro. The results showed that the optimal medium for callus induction was WPM+0.50 mg/L TDZ+0.10 mg/L NAA, and the induction rate of callus was 96.67%. The optional medium for callus proliferation was WPM+0.50 mg/L TDZ and the multiplication coefficient was 4.42. The optimal medium for callus differentiation was WPM+2.00 mg/L 6-BA+0.20 mg/L NAA, and the induction rate reached 100%. The optional medium for adventitious bud proliferation culture was WPM+4.00 mg/L 6-BA, the multiplication coefficient was 3.90, and the cluster buds grew well. The optional rooting medium was WPM+10% coconut water+0.20 mg/L NAA, the rooting rate reached 100%, the root system developed well and the test-tube plantlets grew vigorously. Plantlets were transplanted into the mixture substrate with the volume ratio of perlite and peat soil of 1∶3, the survival rate was 96.67% after 60 days and plants grew well. This study can provide technical support for the large-scale seedling production of A. gigantifolia, as well as theoretical basis and technical support for the protection, seedling breeding, and molecular biology research of this species.

  • Omics & Biotechnology
    WU Fangyuan, CAI Ya, HAO Bingqing, JIA Yanxia, YE Hang, ZHANG Zhaoyuan, MA Jinlin
    Chinese Journal of Tropical Crops. 2023, 44(8): 1542-1550. https://doi.org/10.3969/j.issn.1000-2561.2023.08.003

    A technique system for detecting the genome size of Camellia oleifera by flow cytometry was established to analyze the genome size genetic diversity between the superior lines of C. osmantha var. Yilu and C. vietnamensis var. Hongu 23, and to provide reference for selection of special germplasm and analysis of ploidy difference, based on the key links such as the type of dissociated liquid, the plant of internal reference, the tissue organ of the test material, evaluation indexes such as the nuclear dissociation, the degradation of secondary metabolites, the stability of the cell peak, the clear degree of the main peak, the separation degree of the main and miscellaneous peak, the easy degree of sampling, the difference of the genome size of the internal participating samples, the accuracy of genome size determination and the coefficient of variation. The method was used to determine the genome size of the superior lines of C. osmantha and C. vietnamensis. The disintegrating effect of WPB and mGb was better among common dissociative fluids WPB, Tris-MgCl2, OTTO, mGb. Pean and corn were the favorable internal plants, and young leaf or root tip were the favorable experimental materials. The genome size of the superior lines of C. osmantha was from 5.12 to 9.31 Gb, with an average value of 7.60 Gb, the genome size distribution of the superior lines No. 1, No. 37 and No. 45 was extreme, and it was speculated that there were ploidy differences or interspecific hybridization between the parents. The genome size of superior lines of C. vietnamensis varied from 9.17 to 9.85 Gb, the mean value was 9.47 Gb. There was significant difference in genome size between the superior lines of C. osmantha and C. vietnamensis.