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    Evaluation of Heat Tolerance and Screening the Index for the Assessment of Heat Tolerance in Cultivars of Hydrangea
    LING Rui, DAI Zhongwu, DAI Xiaoyu, WU Chunmei, ZHAI Junwen, ZHENG Zexin, WU Shasha
    Chinese Journal of Tropical Crops    2021, 42 (8): 2209-2218.   DOI: 10.3969/j.issn.1000-2561.2021.08.012
    Abstract372)   HTML871)    PDF(pc) (1981KB)(176)       Save

    High temperature is one of the main limiting factors for the introduction and landscape application of Hydrangea in summer. In order to construct the heat resistance evaluation system of Hydrangea cultivars and select heat resistant cultivars, eight Hydrangea cultivars were used to observe the high temperature semi-lethal temperature (HLT50) and the heat injury indexes (HII) under high temperature stress, and 14 physiological and biochemical indexes were measured after six days of high temperature stress at 44 ℃/33 ℃, after which heat resistance was evaluated by multivariate statistical analysis method. The original 14 indexes were transformed into four independent comprehensive indexes by the principal component analysis, and the comprehensive evaluation value (D value) of each variety was obtained by the subordinate function method. According to D value, the eight Hydrangea cultivars were divided into three groups. The moderate heat resistance cultivars included ‘Essar’ ‘Elbtal’ ‘Komachi’ and ‘Shakespeare’. The heat sensitive cultivars included ‘Tricolor’ and ‘Inspiration’. The heat resistance cultivars included ‘Stockings’ and ‘Corsage’. On the basis of D value, the stepwise regression method was used to set up the variable heat resistance evaluation model with the content of proline (Pro), chlorophyll (Chla+Chlb), carotenoid (Car), chlorophyll a/b value (Chla/Chlb), superoxide dismutase (SOD) activity, relative water content (RWC) and soluble sugar (SS) contents as indicators. In addition, HLT50 and HII of high temperature could also be used as a reliable method to identify the heat resistance of Hydrangea cultivars. This study would provide a theoretical basis for the application and popularization of Hydrangea cultivars in southern urban and rural gardens as well as the breeding of heat-resistant hydrangea cultivars.

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    Cloning, Expression and Subcellular Localization Analysis of MaGA20ox4 and MaGA20ox5 Gene from Banana
    DUAN Yajie, CHEN Jingye, CHEN Jingjing
    Chinese Journal of Tropical Crops    2021, 42 (7): 1805-1816.   DOI: 10.3969/j.issn.1000-2561.2021.07.001
    Abstract187)   HTML119)    PDF(pc) (8422KB)(158)       Save

    Using the false stem of Musa spp. AAA Group ‘8818’ and its mutant ‘8818-1’ as the materials, the gDNA and promoter sequences of MaGA20ox4 and MaGA20ox5 genes were cloned homogeneously, and the sequence differences in the genome-sequencing variety, ‘8818’ and ‘8818-1’ were compared. The results of sequence alignment showed that the gDNA similarity of MaGA20ox4 and MaGA20ox5 genes reached 93.82%, while the promoter sequence similarity of the two genes was only 40%. There were large differences in gDNA and promoter sequences of MaGA20ox4 and MaGA20ox5 genes between Williams varieties and genome-sequencing variety, especially the promoter sequence. The gDNA of MaGA20ox4 gene of Williams ‘8818’ and ‘8818-1’ also had difference in base sequence, but the promoter sequence was basically the same, and the result of MaGA20ox5 was similar to MaGA20ox4. Promoter analysis suggested that the promoters of the two genes belonged to inducible promoters, and there were more light response and hormone response elements. The subcellular localization vectors of MaGA20ox4 and MaGA20ox5 were further constructed. The localization results showed that MaGA20ox4 and MaGA20ox5 proteins were localized on the nucleus and might be localized on the cell membrane or cytoplasm. Quantitative RT-PCR results at different developmental stages of different banana fruits showed that MaGA20ox4 and MaGA20ox5 were species-specific, and may not be the main gene regulating gibberellin synthesis in Williams banana fruits.

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