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    Evaluation of Drought Resistance on Twenty Sugarcane Varieties (Strains)
    LI Xiaojun, LUO Zhengqing, LU Changqiang, TANG Jichang, ZHOU Zhong, CAO Qi
    Chinese Journal of Tropical Crops    2020, 41 (12): 2482-2491.   DOI: 10.3969/j.issn.1000-2561.2020.12.016
    Abstract507)   HTML15)    PDF(pc) (1755KB)(151)       Save

    Drought is the main reason to limit the increase of sugarcane yield in China. The drought resistance of 20 sugarcane varieties (strains) selected from Lincang, the main sugarcane planting area in Yunnan, was analyzed under pot and artificial water control conditions. Eight physiological indexes of stress tolerance at seedling and tillering stage, and tillering rate, survival rate and plant height were determined. Drought resistance of the sugarcane varieties was evaluated by fuzzy membership function, principal component analysis and systematic cluster analysis. After drought stress, the relative water content and chlorophyll content of sugarcane leaves, the tiller rate, plant height and survival rate decreased significantly, while the content of malondialdehyde, proline and soluble sugar, plasma membrane permeability, enzyme activities of SOD and POD showed an increasing trend. 20 sugarcane varieties (strains) could be divided into 3 categories through fuzzy membership function, principal component analysis and systematic cluster analysis, including 7 drought-resistant varieties (strains), 6 moderately drought-resistant varieties/strains and 7 drought-sensitive varieties (strains). The 7 drought-resistant varieties (strains) ranked as ‘GT06-2081’> ‘LC05-136’> ‘FN38’> ‘LC03-1137’ >‘DZ03-83’ >‘YZ05-49’ >‘FN40’ according to the comprehensive value. Correlation analysis showed that survival rate, plant height and relative water content of leaves were significantly positively correlated with drought resistance, while plasma membrane permeability, proline and soluble sugar content were significantly negatively correlated with drought resistance.

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    Complete Sequencing and Analysis of Chloroplast Genome from Alpinia officinarum Hance
    HUANG Qionglin
    Chinese Journal of Tropical Crops    2021, 42 (1): 1-6.   DOI: 10.3969/j.issn.1000-2561.2021.01.001
    Abstract294)   HTML18)    PDF(pc) (2223KB)(184)       Save

    To explore the chloroplast genome features and phylogenetic relationship of Alpinia officinarum Hance, total DNA of A. officinarum was used to sequence by NovaSeq, the high-throughput sequencing platform. And then the chloroplast genome of A. officinarum was annotated and analyzed based on bioinformatics. The results showed that the chloroplast genome of A. officinarum exhibited a typical four-stage structure with a length of 162 137 bp and was composed of a large single-copy region (87 264 bp), a small single-copy region (15 349 bp) and two inverted repeat regions (29 762 bp). A total of 132 genes were annotated in the chloroplast genome of A. officinarum, including 86 protein-coding genes, 8 rRNA genes and 38 tRNA genes. The codon preference in the chloroplast genome was weak, and the codon tended to end with A/T bases. The codon replacement in the majority of coding genes did not lead to the alteration of amino acids. Additionally, phylogenetic analysis in the chloroplast genome of 20 species revealed A. officinarum shared closer relationship with A. zerumbet and A. oxyphylla. Collectively, this study reported the characteristic information of A. officinarum chloroplast genome, which would provide a firm foundation for the researches on genetic evolution and variety breeding of A. officinarum.

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