Construction of Microemulsion System of Macadamia Oil

doi:10.3969/j.issn.1000-2561.2019.02.021

Abstract

Abstract:

In order to expand the application area of macadamia oil, the construction of macadamia oil microemulsion system was researched. The microemulsion area was used as the evaluation index when types and dosages of surfactants and cosurfactants and preparation temperature were optimized by the pseudoternary phase diagrams method combined with single factor experiments and the Box-Behnken experiment. In order to evaluate its stability, the particle sizes of two microemulsion formulations near the center of two dilution lines in the O/W microemulsion center region were measured. The results showed that the better formula for macadamia oil microemulsion were obtained as follows: 2.5 mass ratio of Tween 60 to PEG-40 acted as a mixed surfactants, 3.5 mass ratio of PEG 400 to macadamia oil acted as a mixed oil phase, preparation temperature 60 ℃. Microemulsion with large area, colorless, transparent and infinite dilution could be prepared, the S_ME was 13.61%. In the process of long-term storage and mass dilution, little change was observed in the particle size of microemulsion and thus showed better stability.

Key words: macadamia oil, microemulsion area, Box-Behnken experiment, particle size, stability

CLC Number:

TS201.2

TU Xinghao,ZHANG Shuaizhong,TANG Jinghua,DU Liqing,SHUAI Xixiang,ZHANG Ming. Construction of Microemulsion System of Macadamia Oil[J]. Chinese Journal of Tropical Crops, 2019, 40(2): 359-367.

Figures/Tables 16

References 25

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水平 Levels	因素 Factors
水平 Levels	TW-60/PEG-40质量比 (A) Mass ratio of TW-60 to PEG-40	PEG400/坚果油质量比 (B) Mass ratio of PEG400 to oil	温度(C) Temperature /℃
-1	1∶1	2∶1	45
0	3∶1	3∶1	60
1	5∶1	4∶1	75

水平 Levels	因素 Factors
水平 Levels	TW-60/PEG-40质量比 (A) Mass ratio of TW-60 to PEG-40	PEG400/坚果油质量比 (B) Mass ratio of PEG400 to oil	温度(C) Temperature /℃
-1	1∶1	2∶1	45
0	3∶1	3∶1	60
1	5∶1	4∶1	75

表面活性剂 Surfactants	DR（质量比） DR (mass ratio)	外观 Exterior	是否成乳 Emulsion Yes/No
PGPR	-	分层,上层浅黄色,下层半透明	否
SP-80	-	不透明淡黄色乳液	否
TW-20	-	不透明白色乳液	否
TW-60	7∶3	淡黄色,澄清透明	是
TW-80	8∶2	浅黄色,澄清透明	是
PEG-40	6∶4	无色透明,流动性略差	是

表面活性剂 Surfactants	DR（质量比） DR (mass ratio)	外观 Exterior	是否成乳 Emulsion Yes/No
PGPR	-	分层,上层浅黄色,下层半透明	否
SP-80	-	不透明淡黄色乳液	否
TW-20	-	不透明白色乳液	否
TW-60	7∶3	淡黄色,澄清透明	是
TW-80	8∶2	浅黄色,澄清透明	是
PEG-40	6∶4	无色透明,流动性略差	是

方差来源 Sources of variation	平方和 Sum of squares	自由度 Degree of freedom	均方 Mean square	F值 F-values	p值 p-values	显著性 Significance
模型Model	111.34	9	12.37	13.23	0.0013	**
A	3.61	1	3.61	3.86	0.0900
B	46.99	1	46.99	50.25	0.0002	**
C	5.19	1	5.19	5.55	0.0506
AB	1.31	1	1.31	1.40	0.2760
AC	0.05	1	0.05	0.05	0.8251
BC	4.63×10^-5	1	4.63×10^-5	4.95×10^-5	0.9946
A²	19.36	1	19.36	20.70	0.0026	**
B²	17.86	1	17.86	19.10	0.0033	**
C²	11.36	1	11.36	12.15	0.0102	*
残差Residual	6.55	7	0.94	6.55
失拟项Lack of fit	5.34	3	1.78	5.89	0.0598
纯误差Pure Error	1.21	4	0.30
总和Total sum	117.89	16