Cloning and Expression Analysis of Stress-induced-Phosphoprotein 1 (<i>FoSTIP1</i>) in <i>Fusarium oxysporum</i> f. sp. <i>cubense race</i> 4
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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 341-347.DOI: 10.3969/j.issn.1000-2561.2019.02.019

• Plant Pathology, Crop Pests, Diseases and Their Control • Previous Articles     Next Articles

Cloning and Expression Analysis of Stress-induced-Phosphoprotein 1 (FoSTIP1) in Fusarium oxysporum f. sp. cubense race 4

QI Xingzhu1,2,WANG Jun3,LIU Lei3   

  1. 1. Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan University, Haikou, Hainan 570228, China
    2. College of Ocean, Hainan University, Haikou, Hainan 570228, China
    3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2018-06-27 Revised:2018-09-30 Online:2019-02-25 Published:2019-05-16

Abstract:

In this study, a putative target gene of FoSkn7, a transcription factor in Fusarium oxysporum f. sp. cubense race 4 (Foc4) was cloned. The result showed that the ORF sequence was 1737 nt and encoded a protein containing 578 amino acids. Domain and phylogenetic analysis showed that the protein contained a STI1 domain and several tetratricopeptide repeat (TPR) domains. The protein had the highest similarity with the stress-induced-phosphoprotein 1 of F. oxysporum f. sp. lycopersici 4287, indicating that the protein was STIP1 in Foc4 and named FoSTIP1. To analyze the expression of FoSTIP1 in Foc4 during invasion of the roots of banana seedling sorbeing induced by H2O2, the real time RT-PCR was performed. The results showed that the expression of FoSTIP1 was significantly up-regulated under these two conditions. The different expression of FoSTIP1 between wild-type B2 strain and FoSKN7 deletion mutant were also analyzed by the real time RT-PCR after being induced by H2O2. The result showed that the expression of FoSTIP1 in FoSKN7 deletion mutant was much lower than that of FoSTIP1 in B2 strain, even it was induced by H2O2. The results suggested that the putative target gene of FoSkn7 may be involved in the process of Foc4 resistance to exogenous oxidative stress.

Key words: Foc4, STIP1, expression characteristics

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