Isolation and Identification of Leaf Pathogen from Pandanus amaryllifolius Roxb.

doi:10.3969/j.issn.1000-2561.2022.12.015

Abstract

Abstract:

Pandanus amaryllifolius, a new food flavor, is loved by consumers. The main edible part is leaves. Leaf diseases have become the primary restrictive factor affecting the development of P. amaryllifolius industry. There are few reports on the research of leaf diseases on P. Amaryllifolius. In order to control the pathogens causing leaf diseases, this study investigated the occurrence and epidemic of leaf diseases, defined the main incidence period and disease types, isolated and identified the pathogens of different leaf diseases, and it would provide technical support for promoting the development of P. amaryllifolius industry. The occurrence of leaf diseases mainly began from the middle and late November of each year to the end of April of the next year. The pathogenicity of 28 isolated pathogens was determined in vitro leaves and in vivo potted seedlings. Through the observation of pathogen colony morphology, spore microstructure, universal identification of fungal primers ITS1/ITS4 and pathogenicity determination, strains BDC4121, BDC11221, BDC4112, BDC21112, XYS211, LSS112, LSS214, LSS213 and LSS221 were finally obtained through Koch’s rule verification. Through the screening of sporulation medium, BDC11221 was found to produce spores in mung bean liquid medium and shaking table for 5-7 days, LSS214 to spores in PDA at 28℃ for about 30 days, and BDC4121, BDC4112, XYS211, LSS112, LSS221 and LSS213 to produce spores in PDA at 28℃ for 7-10 days. Combined with ITS1/ITS4 identification and spore microstructure observation, the pathogens were in Talaromyces, Fusarium, Colletotrichum, Epicoccum, Pestalotiopsis, Phomopsis, Alternaria and Acrocalymma. The pathogens will be clarified with multigenome sequencing technology.

Key words: Pandanus amaryllifolius, pathogen of leaf diseases, isolation, identification

CLC Number:

S567.239

GOU Yafeng, XUE Chao, GAO Shengfeng, SUN Shiwei, QIN Xiaowei, YU Huan, TIAN Tian, LIU Shichao. Isolation and Identification of Leaf Pathogen from Pandanus amaryllifolius Roxb.[J]. Chinese Journal of Tropical Crops, 2022, 43(12): 2527-2533.

Figures/Tables 4

References 22

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菌株编号 Strain code	菌落形态 Colony morphology
BDC4121	菌落正面白色、背面浅黄色，菌丝致密很难挑起，菌落长至后期出现黑色分生孢子盘
BDC11221	菌落菌丝丰富、白色，背面玫红色，菌落长至后期中央出现黄色菌丝
BDC4112	菌落灰白色，菌落长至后期菌落呈水渍状，边缘不整齐
BDC21112	菌落中央绒毛状菌丝白色，有黄褐色的色素渗入培养基中，菌落背面黄褐色，边缘浅褐色
XYS211	菌落灰色，菌丝浓密，边缘整齐，近圆形
LSS112	菌落白色，菌落花瓣状生长，中央同心环状微隆起，边缘不规则，菌落长至后期中心有黑色分生孢子盘产生
LSS214	菌落白色，菌丝放射状生长，菌落上不平整，菌落长至后期出现黑色分生孢子盘
LSS213	菌落棕红色，边缘一圈纯白色，菌丝绒毛状致密，菌落近圆形，边缘整齐
LSS221	菌落正面灰色、背面灰褐色，边缘有三角形凹陷，菌落长至后期菌丝变短成为黑色

菌株编号 Strain code	菌落形态 Colony morphology
BDC4121	菌落正面白色、背面浅黄色，菌丝致密很难挑起，菌落长至后期出现黑色分生孢子盘
BDC11221	菌落菌丝丰富、白色，背面玫红色，菌落长至后期中央出现黄色菌丝
BDC4112	菌落灰白色，菌落长至后期菌落呈水渍状，边缘不整齐
BDC21112	菌落中央绒毛状菌丝白色，有黄褐色的色素渗入培养基中，菌落背面黄褐色，边缘浅褐色
XYS211	菌落灰色，菌丝浓密，边缘整齐，近圆形
LSS112	菌落白色，菌落花瓣状生长，中央同心环状微隆起，边缘不规则，菌落长至后期中心有黑色分生孢子盘产生
LSS214	菌落白色，菌丝放射状生长，菌落上不平整，菌落长至后期出现黑色分生孢子盘
LSS213	菌落棕红色，边缘一圈纯白色，菌丝绒毛状致密，菌落近圆形，边缘整齐
LSS221	菌落正面灰色、背面灰褐色，边缘有三角形凹陷，菌落长至后期菌丝变短成为黑色

菌株编号 Strain code	学名 Scientific name	最高分 Maximum score	总分 Total score	查询覆盖率 Query coverage/%	相似度 Similarity/%	索引 Accession
BDC4121	篮状菌属Talaromyces	1064	6333	100	100.00	CP017345.1
BDC11221	镰刀菌属Fusarium	1005	1005	100	100.00	MT322117.1
BDC4112	炭疽菌属Colletotrichum	1026	1026	100	100.00	MW412624.1
XYS211	炭疽菌属Colletotrichum	1016	1016	100	100.00	MT587807.1
BDC21112	附球菌属Epicoccum	749	749	100	100.00	KC178650.1
LSS112	拟盘多毛孢菌属Pestalotiopsis	963	963	100	99.81	MT597834.1
LSS214	拟茎点霉属Phomopsis	1018	1018	100	100.00	MT355680.1
LSS221	链格孢属Alternaria	1000	1000	100	100.00	MT554514.1
LSS213	Acrocalymma	854	854	89	99.79	MT185556.1

菌株编号 Strain code	学名 Scientific name	最高分 Maximum score	总分 Total score	查询覆盖率 Query coverage/%	相似度 Similarity/%	索引 Accession
BDC4121	篮状菌属Talaromyces	1064	6333	100	100.00	CP017345.1
BDC11221	镰刀菌属Fusarium	1005	1005	100	100.00	MT322117.1
BDC4112	炭疽菌属Colletotrichum	1026	1026	100	100.00	MW412624.1
XYS211	炭疽菌属Colletotrichum	1016	1016	100	100.00	MT587807.1
BDC21112	附球菌属Epicoccum	749	749	100	100.00	KC178650.1
LSS112	拟盘多毛孢菌属Pestalotiopsis	963	963	100	99.81	MT597834.1
LSS214	拟茎点霉属Phomopsis	1018	1018	100	100.00	MT355680.1
LSS221	链格孢属Alternaria	1000	1000	100	100.00	MT554514.1
LSS213	Acrocalymma	854	854	89	99.79	MT185556.1