Isolation and Identification of Leaf Pathogen from <i>Pandanus amaryllifolius</i> Roxb.
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2022, Vol. 43 ›› Issue (12): 2527-2533.DOI: 10.3969/j.issn.1000-2561.2022.12.015

• Plant Protection & Bio-safety • Previous Articles     Next Articles

Isolation and Identification of Leaf Pathogen from Pandanus amaryllifolius Roxb.

GOU Yafeng1,2, XUE Chao1, GAO Shengfeng1,2, SUN Shiwei1,2,*(), QIN Xiaowei1,2, YU Huan1,2, TIAN Tian1, LIU Shichao1   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
    2. Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
  • Received:2022-01-26 Revised:2022-06-01 Online:2022-12-25 Published:2023-01-12
  • Contact: SUN Shiwei

Abstract:

Pandanus amaryllifolius, a new food flavor, is loved by consumers. The main edible part is leaves. Leaf diseases have become the primary restrictive factor affecting the development of P. amaryllifolius industry. There are few reports on the research of leaf diseases on P. Amaryllifolius. In order to control the pathogens causing leaf diseases, this study investigated the occurrence and epidemic of leaf diseases, defined the main incidence period and disease types, isolated and identified the pathogens of different leaf diseases, and it would provide technical support for promoting the development of P. amaryllifolius industry. The occurrence of leaf diseases mainly began from the middle and late November of each year to the end of April of the next year. The pathogenicity of 28 isolated pathogens was determined in vitro leaves and in vivo potted seedlings. Through the observation of pathogen colony morphology, spore microstructure, universal identification of fungal primers ITS1/ITS4 and pathogenicity determination, strains BDC4121, BDC11221, BDC4112, BDC21112, XYS211, LSS112, LSS214, LSS213 and LSS221 were finally obtained through Koch’s rule verification. Through the screening of sporulation medium, BDC11221 was found to produce spores in mung bean liquid medium and shaking table for 5-7 days, LSS214 to spores in PDA at 28℃ for about 30 days, and BDC4121, BDC4112, XYS211, LSS112, LSS221 and LSS213 to produce spores in PDA at 28℃ for 7-10 days. Combined with ITS1/ITS4 identification and spore microstructure observation, the pathogens were in Talaromyces, Fusarium, Colletotrichum, Epicoccum, Pestalotiopsis, Phomopsis, Alternaria and Acrocalymma. The pathogens will be clarified with multigenome sequencing technology.

Key words: Pandanus amaryllifolius, pathogen of leaf diseases, isolation, identification

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