Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 269-274.DOI: 10.3969/j.issn.1000-2561.2019.02.009

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Exogenous Trehalose Modulating the Tolerance of Osmotic Stress in Watermelon Cells

SUN Mengli1,WANG Shihao1,XU Zijian1,JIANG Xuefei1,*(),SUN Huapeng2,QIAO Fei2,CONG Hanqing2,YU Ping3   

  1. 1. College of Horiticulture, Hainan University / Hainan Key Laboratory of Sustainable Utilization of Tropical Biological Resources, Haikou, Hainan 570228, China
    2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
    3. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 613319, China
  • Received:2018-07-03 Revised:2018-08-21 Online:2019-02-25 Published:2019-05-16
  • Contact: JIANG Xuefei


In order to explore the effect of exogenous trehalose on watermelon cells under osmotic stress, watermelon suspension culture cells were used as the materials in this study. The changes of cell growth, extracellular pH, intracellular ROS content, and microtubule skeletons under mannitol stress in presence of exogenous trehalose were measured. The results showed that osmotic stress could inhibit cell growth, induce extracellular alkalization, ROS bursting, and counteract the depolymerization of microtubular cytoskeleton. However, exogenous trehalose could alleviate the inhibitory effect on cell growth caused by osmotic stress, suppress the expression of extracellular alkalization and ROS, and maintain the integrity of the microtubular cytoskeleton. The above results indicated that trehalose had protective effects on watermelon cell growth by maintaining subcellular structure and tuning stress related signals against osmotic stress.

Key words: watermelon, osmotic stress, trehalose, ROS, microtubular cytoskeleton

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