Exogenous Trehalose Modulating the Tolerance of Osmotic Stress in Watermelon Cells

doi:10.3969/j.issn.1000-2561.2019.02.009

Abstract

Abstract:

In order to explore the effect of exogenous trehalose on watermelon cells under osmotic stress, watermelon suspension culture cells were used as the materials in this study. The changes of cell growth, extracellular pH, intracellular ROS content, and microtubule skeletons under mannitol stress in presence of exogenous trehalose were measured. The results showed that osmotic stress could inhibit cell growth, induce extracellular alkalization, ROS bursting, and counteract the depolymerization of microtubular cytoskeleton. However, exogenous trehalose could alleviate the inhibitory effect on cell growth caused by osmotic stress, suppress the expression of extracellular alkalization and ROS, and maintain the integrity of the microtubular cytoskeleton. The above results indicated that trehalose had protective effects on watermelon cell growth by maintaining subcellular structure and tuning stress related signals against osmotic stress.

Key words: watermelon, osmotic stress, trehalose, ROS, microtubular cytoskeleton

CLC Number:

Q945.78

SUN Mengli,WANG Shihao,XU Zijian,JIANG Xuefei,SUN Huapeng,QIAO Fei,CONG Hanqing,YU Ping. Exogenous Trehalose Modulating the Tolerance of Osmotic Stress in Watermelon Cells[J]. Chinese Journal of Tropical Crops, 2019, 40(2): 269-274.

Figures/Tables 4

Fig. 1 Effects of exogenous trehalose on growth of watermelon suspension culturedcells under osmotic stress Different catipal letters show extremely significant differences (p<0.01); different lowercase letters show significant differences (p<0.05).

Fig. 2 Effect of exogenous trehalose on extracellular pH of watermelon suspension cultured cells under osmotic stress

Fig. 3 Effect of exogenous trehalose on reactive fluorescence intensity of reactive oxygen species in watermelon cells under osmotic stress A, B, C: Bright field pictures;D, E, F: fluorescence microscopy images; Scale bar=20 μm.

Fig. 4 Effect of exogenous trehalose on the microtubule skeleton of watermelon cells under osmotic stress A: Control; B: Treated by 100 mmol/L mannitol for 1 h; C: Pretreated with 10 mmol/L trehalose for 12 h, then challenged by 100 mmol/L mannitol for 1 h. Scale bar=20 μm.

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