Chinese Journal of Tropical Crops ›› 2021, Vol. 42 ›› Issue (10): 2952-2957.DOI: 10.3969/j.issn.1000-2561.2021.10.027
• Plant Protection & Bio-safety • Previous Articles Next Articles
SHAN Jinxue, LI Zengping*(), ZHANG Yu, WANG Xiaoyu, HU Zhenzhen, ZHU Qingliang, CHEN Xianlei
Received:
2020-12-15
Revised:
2021-02-03
Online:
2021-10-25
Published:
2021-11-25
Contact:
LI Zengping
CLC Number:
SHAN Jinxue, LI Zengping, ZHANG Yu, WANG Xiaoyu, HU Zhenzhen, ZHU Qingliang, CHEN Xianlei. Identification and Biological Characteristics of Pathogen of Stem Rot of Broussonetia papyrifera[J]. Chinese Journal of Tropical Crops, 2021, 42(10): 2952-2957.
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片段 Fragment | 引物名称 Primer | 引物碱基序列(5'-3') DNA sequences of the primer (5'-3') | 参考文献 Ref. |
---|---|---|---|
ITS | ITS1 | TCCGTAGGTGAACCTGCGG | 文庭池等[ |
ITS4 | TCCTCCGCTTATTGATATGC | ||
SSU | NS1 | GTAGTCATATGCTTGTCTC | Hibbett等[ |
NS4 | CTTCCGTCAATTCCTTTAAG | ||
LSU | LROR | AGATGAAAAGAACTTTGAAAAGAGAG | 谭秀梅等[ |
LR5 | ATCCTGAGGGAAACTTC |
Tab. 1 Primers used for DNA sequences
片段 Fragment | 引物名称 Primer | 引物碱基序列(5'-3') DNA sequences of the primer (5'-3') | 参考文献 Ref. |
---|---|---|---|
ITS | ITS1 | TCCGTAGGTGAACCTGCGG | 文庭池等[ |
ITS4 | TCCTCCGCTTATTGATATGC | ||
SSU | NS1 | GTAGTCATATGCTTGTCTC | Hibbett等[ |
NS4 | CTTCCGTCAATTCCTTTAAG | ||
LSU | LROR | AGATGAAAAGAACTTTGAAAAGAGAG | 谭秀梅等[ |
LR5 | ATCCTGAGGGAAACTTC |
种名 Species name | 菌株号 Strain No. | GeneBank登录号GenBank accession number | ||
---|---|---|---|---|
ITS | SSU | LSU | ||
实验菌株 | GSJF001 | MW193047.1 | MW227479.1 | MW227480.1 |
Ganoderma australe | XJSJF003 | MN747808.1 | MN747807.1 | MN744680.1 |
G. australe | YLJF001 | MN689631.1 | MN689632.1 | MN689633.1 |
G. australe | MMHJF001 | MN696209.1 | MN696211.1 | MW131225.1 |
G. sinense | GACP17101260 | MK313126.1 | MK341563.1 | MK336404.1 |
G. sinense | SA17092559 | MK345452.1 | MK346845.1 | MK346834.1 |
G. gibbosum | UB2 | KU569557.1 | KU695658.1 | KU570955.1 |
G. gibbosum | SPC2 | KU569547.1 | KU695655.1 | KU570946.1 |
G. nasalanense | LPDR17060211 | MK345441.1 | MK346842.1 | MK346831.1 |
G. nasalanense | LPDR17060212 | MK345442.1 | MK346843.1 | MK346832.1 |
G. lucidum | FK2580 | MT225392.1 | MT225549.1 | MT225545.1 |
G. tropicum | Loe31 | MK007291.1 | MK235148.1 | MK215781.1 |
G. orbiforme | LPDR14061420 | MK345447.1 | MK346844.1 | MK346833.1 |
Phellinus nigricans | T.Niemela9065 | MF319077.1 | MF319008.1 | MF318934.1 |
Tab. 2 Accession number of sequences in GeneBank
种名 Species name | 菌株号 Strain No. | GeneBank登录号GenBank accession number | ||
---|---|---|---|---|
ITS | SSU | LSU | ||
实验菌株 | GSJF001 | MW193047.1 | MW227479.1 | MW227480.1 |
Ganoderma australe | XJSJF003 | MN747808.1 | MN747807.1 | MN744680.1 |
G. australe | YLJF001 | MN689631.1 | MN689632.1 | MN689633.1 |
G. australe | MMHJF001 | MN696209.1 | MN696211.1 | MW131225.1 |
G. sinense | GACP17101260 | MK313126.1 | MK341563.1 | MK336404.1 |
G. sinense | SA17092559 | MK345452.1 | MK346845.1 | MK346834.1 |
G. gibbosum | UB2 | KU569557.1 | KU695658.1 | KU570955.1 |
G. gibbosum | SPC2 | KU569547.1 | KU695655.1 | KU570946.1 |
G. nasalanense | LPDR17060211 | MK345441.1 | MK346842.1 | MK346831.1 |
G. nasalanense | LPDR17060212 | MK345442.1 | MK346843.1 | MK346832.1 |
G. lucidum | FK2580 | MT225392.1 | MT225549.1 | MT225545.1 |
G. tropicum | Loe31 | MK007291.1 | MK235148.1 | MK215781.1 |
G. orbiforme | LPDR14061420 | MK345447.1 | MK346844.1 | MK346833.1 |
Phellinus nigricans | T.Niemela9065 | MF319077.1 | MF319008.1 | MF318934.1 |
Fig. 1 Field symptoms of B. papyrifera stem rot A: The symptoms of middle period of disease; B: The symptoms of later period of disease; C: Basidiocarp that grow on the stems of diseased trees in the dry season; D: Basidiocarp that grow on the stems of diseased trees in the moist season; E: The basidiocarp and the muddy spore powder on the surface of the stems of diseased trees.
Fig. 2 Pathogenicity determination and basidocarpinduction A: Pathogens have colonized at the inoculation site; B: White rot appeared on the internal tissue; C and D: Basidocarps of Ganoderma that grows after young trees inoculated with pathogens after 2 months; E: CK; F: Isolated strains developed basidiocarps in bottled sawdust medium.
Fig. 3 Morphology of pathogen A: Colony morphology (front side); B: Basidiocarps growing under moist conditions; C: Basidiocarp growing under dry conditions (front side); D: Basidiocarp (back side); E: Basidiospores; F and G: Hyphae (① Bindinghyphae; ② Generative hyphae; ③ Skeletal hyphae).
Fig. 5 Colony diametet of GSJF001 under different treatment conditions Different lowercase letters represent significant difference (P<0.05), different uppercase letters represent extremely significant difference (P<0.01).
培养基 Culture media | 菌落特征Colony characteristics | |
---|---|---|
菌丝生长状态 Mycelium growth status | 菌落直径Colony diameter/cm | |
PDA | 菌丝白色浓密,菌丝长,边缘整齐密集,呈放射状 | 7.27aA |
CA | 菌丝白色较浓密,菌丝较长,边缘整齐密集,呈放射状 | 7.07bB |
PSA | 菌丝白色浓密,菌丝长,边缘平展密集,呈放射状 | 6.80cC |
CMA | 菌丝白色稀疏,菌丝较长,边缘整齐较密集,呈放射状 | 3.80eE |
OMA | 菌丝半透明稀疏,菌丝较长,边缘平展稀疏,呈放射状 | 4.17dD |
Richards | 菌丝半透明稀疏,菌丝长,边缘平展稀疏,呈放射状 | 2.67fF |
枸树茎干汁液 | 菌丝半透明稀疏,菌丝短,边缘整齐稀疏,呈放射状 | 4.07dD |
Tab. 3 Effect of different culture media on GSJF001 mycelia growth
培养基 Culture media | 菌落特征Colony characteristics | |
---|---|---|
菌丝生长状态 Mycelium growth status | 菌落直径Colony diameter/cm | |
PDA | 菌丝白色浓密,菌丝长,边缘整齐密集,呈放射状 | 7.27aA |
CA | 菌丝白色较浓密,菌丝较长,边缘整齐密集,呈放射状 | 7.07bB |
PSA | 菌丝白色浓密,菌丝长,边缘平展密集,呈放射状 | 6.80cC |
CMA | 菌丝白色稀疏,菌丝较长,边缘整齐较密集,呈放射状 | 3.80eE |
OMA | 菌丝半透明稀疏,菌丝较长,边缘平展稀疏,呈放射状 | 4.17dD |
Richards | 菌丝半透明稀疏,菌丝长,边缘平展稀疏,呈放射状 | 2.67fF |
枸树茎干汁液 | 菌丝半透明稀疏,菌丝短,边缘整齐稀疏,呈放射状 | 4.07dD |
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