Analysis of Common Differentially Expressed Genes in Leaf Yellowing of Areca Plam Based on Transcriptome Sequencing

doi:10.3969/j.issn.1000-2561.2021.10.012

Abstract

Abstract:

Areca catechu L. is an important economic crop in Hainan, China, the leaf yellowing has become increasingly serious, which is a major obstacle to the sustainable and healthy development of the areca industry. According to field research, the symptoms of leaf yellowing mainly included specific yellowing from 1/4 of leaf margin and whole leaf yellowing. In this study, 440 differentially co-expressed genes were screened in the specific leaf yellowing group by transcriptome sequencing, which were mainly concentrated in secondary metabolic pathways, hormone signal transduction pathways and energy metabolism pathways. This study explored the molecular mechanism of specific etiolation of areca leaves under different stresses and provided theoretical basis for the comprehensive prevention and control of areca plam yellowing disease.

Key words: Areca catechu L., leaf yellowing, transcriptome sequencing

CLC Number:

S763.7

XUAN Zhe, CHE Haiyan, CAO Xueren, HE Yanheng, LUO Daquan. Analysis of Common Differentially Expressed Genes in Leaf Yellowing of Areca Plam Based on Transcriptome Sequencing[J]. Chinese Journal of Tropical Crops, 2021, 42(10): 2834-2840.

Figures/Tables 8

Tab. 1 qRT-PCR primer design

引物名称 Primer name	碱基序列（5′-3′） Primers sequences (5′-3′)
68503F	GTGATGGGCGTGTCAAGA
68503R	TGCCGAAGAGATACTCGTTC
117057F	TCAAGACTGATTGGAGTAAGGC
117057R	AGTGGTTCCTCACCCATCTC
115124F	GCAGTTGGAATGGGAGAATC
115124R	TTGTGCCTGGAAGGTTGATA
116247F	GAGTATGCGAGGAGGAAAGC
116247R	CCCACCCAAAGTCAGTTCTAT
Actin F	CGTATGAGCAAAGAGATTACTGC
Actin R	GAAGCCAAGATGGACCCTC

Fig. 1 The number of differentially expressed genes and up-down regulation compared with control group (healthy group)

Fig. 2 Venn of differentially expressed genes between control group (healthy group) and sample 1, sample 2,sample 3 and sample 4

Fig. 3 GO annotation of common differentially expressed genes in specific yellowing of Areca leaves

Fig. 4 GO enrichment of differentially expressed genes up-regulated in leaves with specific yellowing symptoms

Fig. 5 GO enrichment of differentially expressed genes down-regulated in leaves with specific yellowing symptoms

Fig. 6 KEGG enrichment of common differentially expressed genes in leaves with specific yellowing symptoms

Fig. 7 Expression analysis of qRT-PCR

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