Interspecific Molecular Phylogeny and Biological Characteristics of Colletotrichum acutatum Complex Species Causing Colletotrichum Leaf Disease on Rubber Trees in China

doi:10.3969/j.issn.1000-2561.2020.09.019

Abstract

Abstract:

In order to clarify the biological characteristics and interspecific differences of Colletotrichum acutatum complex species from rubber trees in China, four representative strains (C. bannanense YNML52, C. laticiphilum YNJH17109, C. australisinense GX1655, YNJP162) were evaluated for the utilization of different carbon, nitrogen sources and colony growth at different temperature, pH and water activities. The pathogenicity of the strains to different rubber varieties and crops was also evaluated. There were significant differences in the utilization of carbon and nitrogen sources, suitable growth temperature, lethal temperature, pH value and water activity of the tested strains. There were significant differences in pathogenicity spectrum of the tested strains. YNML52 had a narrow host range and strong host specificity. Evaluating the effects of ecological factors on the biological characteristics of the pathogen of rubber tree anthracnose is of certain guiding significance for understanding the occurrence regularity of the disease and formulating effective control strategies.

Key words: rubber trees, Colletotrichum acutatum complex species, biological characteristics, pathogenicity

CLC Number:

JIANG Tao,LIU Xianbao,LI Boxun,ZHENG Xiaolan,CAI Jimiao,FENG Yanli,HUANG Guixiu. Interspecific Molecular Phylogeny and Biological Characteristics of Colletotrichum acutatum Complex Species Causing Colletotrichum Leaf Disease on Rubber Trees in China[J]. Chinese Journal of Tropical Crops, 2020, 41(9): 1856-1864.

Figures/Tables 8

Fig. 1 MP phylogenetic tree based on ITS, ACT, GAPDH, CHS-1 and TUB2 sequences

Fig. 2 Effects of carbon source types on mycelial growth of test strains Different capital and lowercase letters mean significant difference at the 0.01 and 0.05 levels, respectively.

Fig. 3 Effects of nitrogen source types on mycelial growth of test strains Different capital and lowercase letters mean significant difference at the 0.01 and 0.05 levels, respectively.

Fig. 4 Effects of different temperatures on mycelial growth of test strains

Fig. 5 Effects of different pH on mycelial growth of test strains

Fig. 6 Effects of different water activities on mycelial growth of test strains

Fig. 7 Lesion size and growth rate on different rubber varieties of test strains Different capital and lowercase letters mean significant difference at the 0.01 and 0.05 levels, respectively.

Fig. 8 Pathogenicity on four crops of tested strains Different lowercase letters mean significant difference at the 0.05 levels, respectively.

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