Exogenous Betaine Modulating the Tolerance of Osmotic Stress in Watermelon Cells

doi:10.3969/j.issn.1000-2561.2020.09.014

Abstract

Abstract:

In order to explore the influence of exogenous glycine betaine on watermelon cell growth under osmotic stress and determine the correlation between the glycine betaine and the watermelon’s osmotic stress resistance, measurement of fresh cell weight, cell volume, intracellular reactive oxygen species (ROS) and extracellular pH was performed with the suspension cultured cells of diploid watermelon as the experimental materials, the mannitol simulating the osmotic stress and the exogenous glycine betaine being applied simultaneously. Under osmotic stress, the fresh weight and growth weight of watermelon cells were both inhibited, and extracellular alkalization and burst of intracellular ROS were also induced. The exogenous glycine betaine was found to be able to alleviate the inhibition effect of osmotic stress on the growth weight of the suspension cultured cells of watermelon to a certain degree, and be of regulative effect on extracellular pH and intracellular ROS level. In a word, the exogenous glycine betaine could maintain the watermelon cell growth and regulate the resistant reaction under osmotic stress.

Key words: watermelon, osmotic stress, betaine, extracellular pH, ROS

CLC Number:

S651

WANG Shihao,ZHU Fangming,SUN Mengli,XU Zijian,JIANG Xuefei,QIAO Fei. Exogenous Betaine Modulating the Tolerance of Osmotic Stress in Watermelon Cells[J]. Chinese Journal of Tropical Crops, 2020, 41(9): 1816-1821.

Figures/Tables 4

Fig. 1 Effect of exogenous trehalose on extracellular pH of watermelon suspension cultured cells under osmotic stress

Fig. 2 Effect of exogenous betaine on reactive oxygen species in watermelon cells under osmotic stress (Scale Bar=20 μm) A、B、C：Bright field pictures;D、E、F：Fluorescence microscopy image.

Fig. 3 Changes of fresh weight in watermelon cells induced by osmotic stress

Fig. 4 Effects of exogenous betaine on growth of watermelon suspension cultured cells under osmotic stress

References 36

[1]	Zhu J K, Hasegawa P M, Bressan R A, et al. Molecular aspects of osmotic stress in plants[J]. Critical Reviews in Plant Sciences, 1997,16(3):253-277. DOI URL
[2]	李刚, 姜晓莉, 董学会. 植物渗透胁迫研究进展[J]. 吉林农业大学学报, 1996(S1):84-86.
[3]	Munns R. Comparative physiology of salt and water stress[J]. Plant Cell and Environment, 2002,25(2):239-250. DOI URL
[4]	Lei Y, Yin C Y, Ren J, et al. Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings[J]. Biologia Plantarum, 2007,51(2):386-390. DOI URL
[5]	Aydi S S, Aydi S, Gonzalez E, et al. Osmotic stress affects water relations, growth, and nitrogen fixation in Phaseolus vulgaris plants[J]. Acta Physiologiae Plantarum, 2008,30(4):441-449. DOI URL
[6]	姚瑞玲. 不同种源青钱柳幼苗对渗透胁迫适应机理的研究[D]. 南京: 南京林业大学, 2007.
[7]	Premecz G, Ruzicska P, Oláh T, et al. Effect of “osmotic stress” on protein and nucleic acid synjournal in isolated tobacco protoplasts[J]. Planta, 1978,141(1):33-36. DOI URL PMID
[8]	马建华, 郑海雷, 赵中秋, 等. 植物抗盐机理研究进展[J]. 生命科学研究, 2001(S1):175-179, 226.
[9]	Rhodes A D, Hanson A D. Quaternary ammonium and tertiary sulfonium compounds in higher plants[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1993,44(1):357-384. DOI URL
[10]	刘友良, 毛才良, 汪良驹. 植物耐盐性研究进展[J]. 植物生理学通讯, 1987(4):1-7.
[11]	Papageorgiou G C, Fujimura Y, Murata N. Protection of the oxygen-evolving photosystem II complex by glycinebetaine[J]. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1991,1057(3):361-366.
[12]	Nomura M, Hibino T, Takabe T, et al. Transgenically produced glycinebetaine protects Ribulose 1, 5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7942 under salt stress[J]. Plant and Cell Physiology, 1998,39(4):425-432. DOI URL
[13]	Chen W P, Li P H, Chen T H H. Glycinebetaine increases chilling tolerance and reduces chilling-induced lipid peroxidation in Zea mays L.[J]. Plant Cell and Environment, 2000,23(6):609-618. DOI URL
[14]	钟国辉, 王建林. 外源甜菜碱对氯化钠胁迫下白菜叶片的保护效应(简报)[J]. 植物生理学通讯, 1997,33(5):333-335.
[15]	郭岩, 张莉, 肖岗, 等. 甜菜碱醛脱氢酶基因在水稻中的表达及转基因植株的耐盐性研究[J]. 中国科学(C辑): 生命科学, 1997,27(2):151-155.
[16]	马千全. 外源甜菜碱提高小麦抗旱性的研究[D]. 泰安: 山东农业大学, 2003.
[17]	Yang X H, Liang Z, Lu C. Genetic engineering of the biosynjournal of glycinebetaine enhances photosynjournal against high temperature stress in transgenic tobacco plants[J]. Plant Physiology, 2005,138(4):2299-2309. DOI URL PMID
[18]	Liang G J. Betaine can improve tolerance to low temperature in plant[J]. Journal of Zhao Qing University, 2003,24(2):36-55.
[19]	Yang X, Lu C. Photosynjournal is improved by exogenous glycinebetaine in salt-stressed maize plants[J]. Physiologia Plantarum, 2005,124(3):343-352. DOI URL
[20]	Ismail A, Riemann M, Nick P. The jasmonate pathway mediates salt tolerance in grapevines[J]. Journal of Experimental Botany, 2012,63(5):2127-2139. DOI URL
[21]	Chang X, Heene E, Qiao F, et al. The phytoalexin resveratrol regulates the initiation of hypersensitive cell death in Vitis cell[J]. PloS One, 2011,6(10):e26405. DOI URL PMID
[22]	Qiao F, Chang X L, Nick P. The cytoskeleton enhances gene expression in the response to the Harpin elicitor in grapevine[J]. Journal of Experimental Botany, 2010,61(14):4021-4031. DOI URL PMID
[23]	刘明乾, 吴绍华, 田郎, 等. 橡胶草悬浮细胞遗传转化体系的建立[J]. 热带生物学报, 2018,9(2):176-182.
[24]	张洪培, 张晓茹, 胡格格, 等. 水杨酸诱发的丹参悬浮培养细胞内H₂O₂迸发与其培养基碱化的关系[J]. 植物科学学报, 2015,33(3):405-413. DOI URL
[25]	Benouaret R, Goujon E, Goupil P. Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells[J]. Plant Physiology and Biochemistry, 2014,77(2):84-89. DOI URL
[26]	Ojalvo I, Rokem J S, Navon G. ³¹P NMR study of elicitor treated Phaseolus vulgaris cell suspension cultures [J]. Plant Physiology, 1987,85(3):716-719. DOI URL PMID
[27]	Okada M, Matsumura M, Ito Y, et al. High-affinity binding proteins for N-acetylchitooligosaccharide elicitor in the plasma membranes from wheat, barley and carrot cells: conserved presence and correlation with the responsiveness to the elicitor[J]. Plant and Cell Physiology, 2002,43(5):505-512. DOI URL PMID
[28]	白英俊, 李国瑞, 黄凤兰, 等. 活性氧与植物抗氧化系统研究进展[J]. 安徽农业科学, 2017,45(36):1-3.
[29]	王海波, 黄雪梅, 张昭其. 植物逆境胁迫中活性氧和钙信号的关系[J]. 北方园艺, 2010(22):189-194.
[30]	张怡, 路铁刚. 植物中的活性氧研究概述[J]. 生物技术进展, 2011,1(4):242-248.
[31]	普凌, 赵鑫, 王艇越, 等. 等渗盐胁迫对番茄幼苗生长和生理特性的影响[J]. 陕西农业科学, 2019,65(5):35-38.
[32]	张腾国, 胡馨丹, 李萍, 等. 盐及低温胁迫对油菜ROS和抗氧化酶活性的影响[J]. 兰州大学学报(自然科学版), 2019,55(4):497-505.
[33]	张士功, 高吉寅, 宋景芝. 甜菜碱对NaCl胁迫下小麦细胞保护酶活性的影响[J]. 植物学通报, 1999(4):429-432.
[34]	杨淑英, 张建新, 吕家珑, 等. 外源甜菜碱对冬小麦抗旱性生理指标的影响研究[J]. 西北植物学报, 2000(6):1041-1045.
[35]	殷云刚, 罗庆熙, 马承, 等. 干旱胁迫下叶面喷施甜菜碱对番茄幼苗生理指标的影响[J]. 北方园艺, 2008(10):60-61.
[36]	马新蕾, 王玉军, 谢胜利, 等. 根施甜菜碱对水分胁迫下烟草幼苗光合机构的保护[J]. 植物生理与分子生物学学报, 2006,32(4):465-472.