Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (9): 1822-1830.DOI: 10.3969/j.issn.1000-2561.2020.09.015
• Plant Cultivation, Physiology & Biochemistry • Previous Articles Next Articles
YANG Wenwu1,2,GUO Dongqin3,CAO Weiguo1,*(),PAN Xingjiao3,XUE Yanbing3,ZHANG Jie3,ZHOU Nong3,*()
Received:
2019-09-10
Revised:
2019-12-31
Online:
2020-09-25
Published:
2020-10-16
Contact:
CAO Weiguo,ZHOU Nong
CLC Number:
YANG Wenwu,GUO Dongqin,CAO Weiguo,PAN Xingjiao,XUE Yanbing,ZHANG Jie,ZHOU Nong. Effects of 27 Strains of Arbuscular Mycorrhizal Fungi Inoculation on Physiology and Biochemistry and Major Components of Terpenoids in Potted Saussurea costus[J]. Chinese Journal of Tropical Crops, 2020, 41(9): 1822-1830.
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Fig. 1 Root infection rate of S. costus seedlings with different AM fungi Different lowercase letters in the each group represent significant difference (P<0.05).
Fig. 2 Antioxidant enzyme content in leaves of S. costus with different AM fungi Different lowercase letters in the each group represent significant difference (P<0.05).
Fig. 3 Content of MDA, soluble sugar and soluble protein in leaves of S. costus with different AM fungi Different lowercase letters in the each group represent significant difference (P<0.05).
组别 Group | 鲜重 Fresh weight/g | 干重 Dry weight/g | 折干率 Drying rate/% |
---|---|---|---|
CK | 3.986±0.744bcd | 0.990±0.129defgh | 29.307±0.010ab |
Ga | 1.255±0.423d | 0.280±0.556i | 22.311±0.093defgh |
Gd | 5.707±0.557bc | 1.390±0.102cd | 24.357±0.012bcdefg |
Gg | 2.493±0.691cd | 0.547±0.514ghi | 21.925±0.064fgh |
Gm | 2.243±0.818d | 0.603±0.354ghi | 26.895±0.105abcdef |
Gr | 1.485±0.043d | 0.425±0.329hi | 28.620±0.054abc |
Sca | 9.640±0.029a | 2.630±0.070b | 27.860±0.052abcdef |
Sdi | 3.070±1.009cd | 0.770±0.202efghi | 25.081±0.112bcdefg |
Spe | 2.808±0.638cd | 0.733±0.252efghi | 26.091±0.055abcdef |
Dh | 6.680±0.212bc | 1.580±0.098c | 23.650±0.063cdefgh |
Rco | 8.820±0.016 a | 2.450±0.081b | 27.780±0.053abcde |
Rfu | 5.005±0.595bcd | 1.270±0.122cde | 25.375±0.018abcdef |
Sde | 14.180±0.010a | 3.990±0.035a | 28.140±0.007abcd |
Svi | 1.400±0.424d | 0.270±0.629i | 19.286±0.088h |
Fm | 2.605±0.453cd | 0.610±0.255ghi | 23.417±0.062cdefgh |
Rcl | 2.710±0.371cd | 0.545±0.257ghi | 20.111±0.008gh |
Rin | 1.930±0.470d | 0.475±0.354hi | 24.611±0.023bcdefg |
Afo | 4.310±0.033bcd | 1.190±0.119cdef | 27.610±0.006abcde |
Ako | 1.370±0.103d | 0.190±0.744i | 13.870±0.007ij |
Asc | 4.980±0.565bc | 1.530±0.120cd | 29.223±0.010a |
Asp | 5.573±0.394bc | 1.430±0.089cd | 25.658±0.013abcdef |
De | 1.930±0.015d | 0.300±0.660i | 15.541±0.022i |
Ds | 2.760±0.410cd | 0.735±0.210efghi | 26.630±0.108abcdef |
Ec | 4.420±0.401bcd | 1.180±0.144cdef | 26.697±0.056abcdef |
Pb | 2.970±0.010cd | 0.670±0.211fghi | 22.560±0.128efgh |
Po | 3.945±0.905bcd | 0.450±0.440hi | 11.407±0.050j |
Ale | 3.040±0.009cd | 0.680±0.250fghi | 22.370±0.065efgh |
Atr | 3.995±0.270bcd | 1.100±0.129cdefg | 28.241±0.007abcd |
Tab. 1 Root biomass of S. costus with different AM fungi
组别 Group | 鲜重 Fresh weight/g | 干重 Dry weight/g | 折干率 Drying rate/% |
---|---|---|---|
CK | 3.986±0.744bcd | 0.990±0.129defgh | 29.307±0.010ab |
Ga | 1.255±0.423d | 0.280±0.556i | 22.311±0.093defgh |
Gd | 5.707±0.557bc | 1.390±0.102cd | 24.357±0.012bcdefg |
Gg | 2.493±0.691cd | 0.547±0.514ghi | 21.925±0.064fgh |
Gm | 2.243±0.818d | 0.603±0.354ghi | 26.895±0.105abcdef |
Gr | 1.485±0.043d | 0.425±0.329hi | 28.620±0.054abc |
Sca | 9.640±0.029a | 2.630±0.070b | 27.860±0.052abcdef |
Sdi | 3.070±1.009cd | 0.770±0.202efghi | 25.081±0.112bcdefg |
Spe | 2.808±0.638cd | 0.733±0.252efghi | 26.091±0.055abcdef |
Dh | 6.680±0.212bc | 1.580±0.098c | 23.650±0.063cdefgh |
Rco | 8.820±0.016 a | 2.450±0.081b | 27.780±0.053abcde |
Rfu | 5.005±0.595bcd | 1.270±0.122cde | 25.375±0.018abcdef |
Sde | 14.180±0.010a | 3.990±0.035a | 28.140±0.007abcd |
Svi | 1.400±0.424d | 0.270±0.629i | 19.286±0.088h |
Fm | 2.605±0.453cd | 0.610±0.255ghi | 23.417±0.062cdefgh |
Rcl | 2.710±0.371cd | 0.545±0.257ghi | 20.111±0.008gh |
Rin | 1.930±0.470d | 0.475±0.354hi | 24.611±0.023bcdefg |
Afo | 4.310±0.033bcd | 1.190±0.119cdef | 27.610±0.006abcde |
Ako | 1.370±0.103d | 0.190±0.744i | 13.870±0.007ij |
Asc | 4.980±0.565bc | 1.530±0.120cd | 29.223±0.010a |
Asp | 5.573±0.394bc | 1.430±0.089cd | 25.658±0.013abcdef |
De | 1.930±0.015d | 0.300±0.660i | 15.541±0.022i |
Ds | 2.760±0.410cd | 0.735±0.210efghi | 26.630±0.108abcdef |
Ec | 4.420±0.401bcd | 1.180±0.144cdef | 26.697±0.056abcdef |
Pb | 2.970±0.010cd | 0.670±0.211fghi | 22.560±0.128efgh |
Po | 3.945±0.905bcd | 0.450±0.440hi | 11.407±0.050j |
Ale | 3.040±0.009cd | 0.680±0.250fghi | 22.370±0.065efgh |
Atr | 3.995±0.270bcd | 1.100±0.129cdefg | 28.241±0.007abcd |
组别 Group | 木香烃内酯 Costunolide | 去氢木香内酯 Dehydrocostuslactone | 总内酯 Total lactones | 结构比(木香烃内酯∶去氢木香内酯) Structure ratio(Costunolide∶Dehydrocostuslactone) |
---|---|---|---|---|
CK | 1.218±0.020no | 19.833±0.037k | 21.050±0.056j | 1.000∶16.289 |
Ga | 0.698±0.126op | 11.098±0.138k | 11.795±0.137j | 1.000∶15.904 |
Gd | 6.142±0.004fg | 90.141±0.007b | 96.283±0.007b | 1.000∶14.677 |
Gg | 4.832±0.176ij | 42.881±0.170ghi | 47.713±0.170fg | 1.000∶8.875 |
Gm | 2.530±0.141kl | 37.085±0.143hij | 39.616±0.143ghi | 1.000∶14.657 |
Gr | 1.553±0.046mn | 32.484±0.032ij | 34.037±0.032hi | 1.000∶20.923 |
Sca | 7.834±0.021c | 92.184±0.022b | 100.018±0.022b | 1.000∶12.037 |
Sdi | 4.656±0.016j | 49.164±0.020fg | 53.820±0.020ef | 1.000∶10.559 |
Spe | 2.229±0.050klm | 42.619±0.035gih | 44.849±0.036fgh | 1.000∶19.117 |
Dh | 4.420±0.004j | 54.735±0.010ef | 59.155±0.009de | 1.000∶12.383 |
Rco | 10.194±0.007a | 90.380±0.004b | 100.574±0.005b | 1.000∶8.866 |
Rfu | 4.357±0.029j | 60.725±0.033de | 65.082±0.033d | 1.000∶13.938 |
Sde | 6.924±0.097de | 105.056±0.074a | 111.979±0.076a | 1.000∶15.174 |
Svi | 1.229±0.148no | 16.639±0.154k | 17.868±0.154j | 1.000∶13.541 |
Fm | 1.501±0.045mn | 38.062±0.034hij | 39.563±0.035ghi | 1.000∶25.361 |
Rcl | 9.211±0.006b | 86.063±0.011b | 95.274±0.010b | 1.000∶9.344 |
Rin | 1.814±0.012mn | 30.409±0.018j | 32.223±0.018i | 1.000∶16.767 |
Afo | 2.302±0.205klm | 65.295±0.192cd | 67.597±0.193d | 1.000∶28.366 |
Ako | 0.431±0.000p | 9.319±0.000k | 9.750±0.000j | 1.000∶21.603 |
Asc | 7.053±0.042d | 72.684±0.037c | 79.737±0.038c | 1.000∶10.305 |
Asp | 5.736±0.021gh | 58.337±0.007de | 64.073±0.008d | 1.000∶10.170 |
De | 0.740±0.042op | 11.548±0.033k | 12.288±0.033j | 1.000∶15.607 |
Ds | 2.153±0.060klm | 35.931±0.060hij | 38.084±0.060ghi | 1.000∶16.689 |
Ec | 5.317±0.026hi | 84.778±0.029b | 90.095±0.029b | 1.000∶15.945 |
Pb | 6.403±0.001ef | 109.595±0.020a | 115.998±0.019a | 1.000∶17.116 |
Po | 1.814±0.097lmn | 46.777±0.065fgh | 48.590±0.066fg | 1.000∶25.793 |
Ale | 1.698±0.099mn | 18.262±0.107k | 19.960±0.107j | 1.000∶10.756 |
Atr | 2.686±0.048k | 43.639±0.056ghi | 46.325±0.056fg | 1.000∶16.249 |
Tab. 2 Content and structure ratio of sesquiterpene lactone in S. costus with different AM fungi mg/g
组别 Group | 木香烃内酯 Costunolide | 去氢木香内酯 Dehydrocostuslactone | 总内酯 Total lactones | 结构比(木香烃内酯∶去氢木香内酯) Structure ratio(Costunolide∶Dehydrocostuslactone) |
---|---|---|---|---|
CK | 1.218±0.020no | 19.833±0.037k | 21.050±0.056j | 1.000∶16.289 |
Ga | 0.698±0.126op | 11.098±0.138k | 11.795±0.137j | 1.000∶15.904 |
Gd | 6.142±0.004fg | 90.141±0.007b | 96.283±0.007b | 1.000∶14.677 |
Gg | 4.832±0.176ij | 42.881±0.170ghi | 47.713±0.170fg | 1.000∶8.875 |
Gm | 2.530±0.141kl | 37.085±0.143hij | 39.616±0.143ghi | 1.000∶14.657 |
Gr | 1.553±0.046mn | 32.484±0.032ij | 34.037±0.032hi | 1.000∶20.923 |
Sca | 7.834±0.021c | 92.184±0.022b | 100.018±0.022b | 1.000∶12.037 |
Sdi | 4.656±0.016j | 49.164±0.020fg | 53.820±0.020ef | 1.000∶10.559 |
Spe | 2.229±0.050klm | 42.619±0.035gih | 44.849±0.036fgh | 1.000∶19.117 |
Dh | 4.420±0.004j | 54.735±0.010ef | 59.155±0.009de | 1.000∶12.383 |
Rco | 10.194±0.007a | 90.380±0.004b | 100.574±0.005b | 1.000∶8.866 |
Rfu | 4.357±0.029j | 60.725±0.033de | 65.082±0.033d | 1.000∶13.938 |
Sde | 6.924±0.097de | 105.056±0.074a | 111.979±0.076a | 1.000∶15.174 |
Svi | 1.229±0.148no | 16.639±0.154k | 17.868±0.154j | 1.000∶13.541 |
Fm | 1.501±0.045mn | 38.062±0.034hij | 39.563±0.035ghi | 1.000∶25.361 |
Rcl | 9.211±0.006b | 86.063±0.011b | 95.274±0.010b | 1.000∶9.344 |
Rin | 1.814±0.012mn | 30.409±0.018j | 32.223±0.018i | 1.000∶16.767 |
Afo | 2.302±0.205klm | 65.295±0.192cd | 67.597±0.193d | 1.000∶28.366 |
Ako | 0.431±0.000p | 9.319±0.000k | 9.750±0.000j | 1.000∶21.603 |
Asc | 7.053±0.042d | 72.684±0.037c | 79.737±0.038c | 1.000∶10.305 |
Asp | 5.736±0.021gh | 58.337±0.007de | 64.073±0.008d | 1.000∶10.170 |
De | 0.740±0.042op | 11.548±0.033k | 12.288±0.033j | 1.000∶15.607 |
Ds | 2.153±0.060klm | 35.931±0.060hij | 38.084±0.060ghi | 1.000∶16.689 |
Ec | 5.317±0.026hi | 84.778±0.029b | 90.095±0.029b | 1.000∶15.945 |
Pb | 6.403±0.001ef | 109.595±0.020a | 115.998±0.019a | 1.000∶17.116 |
Po | 1.814±0.097lmn | 46.777±0.065fgh | 48.590±0.066fg | 1.000∶25.793 |
Ale | 1.698±0.099mn | 18.262±0.107k | 19.960±0.107j | 1.000∶10.756 |
Atr | 2.686±0.048k | 43.639±0.056ghi | 46.325±0.056fg | 1.000∶16.249 |
Fig. 4 Yield of costuslactone in root of A. lappa with different AM fungi Different lowercase letters in the each group represent significant difference (P<0.05).
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