Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 247-253.DOI: 10.3969/j.issn.1000-2561.2019.02.006

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Effect of Endogenous H2O2 on the Germination of Rice Seeds

XIAO Yu,FENG Hongyu,CHEN Huiping()   

  1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received:2018-08-01 Revised:2018-10-15 Online:2019-02-25 Published:2019-05-16
  • Contact: CHEN Huiping


To explore the effect of endogenous hydrogen peroxide (H2O2) on the growth of radicle and plumule, and the apical vitality in the radicle during germination, rice (Oryza sativa L.) seeds were cultured with different concentrations of nicotinamide adenine dinucleotide phosphate (NADPH) synthase inhibitor diphenylene iodonium (DPI) or H2O2 scavenger dimethylthiourea (DMTU). The results showed that the growth of radicle and plumule in the rice seeds cultured with both DMTU and DPI was inhibited, especially that with DPI. Higher concentration of DPI or DMTU resulted in stronger inhibitory effect on rice seed germination. The inhibitory effect of DPI on rice seed germination was more obvious than that of DMTU. In addition, the contents of superoxide anion (O 2-) and H2O2 decreased in the apical of radicle with the increase of DMTU concentration, and the number of damage cell increased. Therefore, It suggested that endogenous H2O2 may be involved in regulating the germination of rice seeds.

Key words: endogenous H2O2, seed germination, DPI, DMTU

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