Effect of Endogenous H2O2 on the Germination of Rice Seeds

doi:10.3969/j.issn.1000-2561.2019.02.006

Abstract

Abstract:

To explore the effect of endogenous hydrogen peroxide (H₂O₂) on the growth of radicle and plumule, and the apical vitality in the radicle during germination, rice (Oryza sativa L.) seeds were cultured with different concentrations of nicotinamide adenine dinucleotide phosphate (NADPH) synthase inhibitor diphenylene iodonium (DPI) or H₂O₂ scavenger dimethylthiourea (DMTU). The results showed that the growth of radicle and plumule in the rice seeds cultured with both DMTU and DPI was inhibited, especially that with DPI. Higher concentration of DPI or DMTU resulted in stronger inhibitory effect on rice seed germination. The inhibitory effect of DPI on rice seed germination was more obvious than that of DMTU. In addition, the contents of superoxide anion (O ^2-) and H₂O₂ decreased in the apical of radicle with the increase of DMTU concentration, and the number of damage cell increased. Therefore, It suggested that endogenous H₂O₂ may be involved in regulating the germination of rice seeds.

Key words: endogenous H₂O₂, seed germination, DPI, DMTU

CLC Number:

S511

XIAO Yu,FENG Hongyu,CHEN Huiping. Effect of Endogenous H₂O₂ on the Germination of Rice Seeds[J]. Chinese Journal of Tropical Crops, 2019, 40(2): 247-253.

Figures/Tables 8

Fig. 1 Germination of rice seeds treated with different concentrations of DPI A: 0 μmol/L DPI; B: 10 μmol/L DPI; C: 20 μmol/L DPI; D: 30 μmol/L DPI; E: 40 μmol/L DPI; F: 50 μmol/L DPI; G: 60 μmol/L DPI.

Fig. 2 Plumule length of germinating rice seeds in the different concentration of DPI Columns with the different lowercase letters are significantly different at p<0.05.

Fig. 3 Germination of rice seeds treated with different concentrations of DMTU A: 0 mmol/L DMTU; B: 10 mmol/L DMTU; C: 20 mmol/L DMTU; D: 30 mmol/L DMTU; E: 40 mmol/L DMTU; F: 50 mmol/L DMTU; G: 60 mmol/L DMTU.

Fig. 4 Radicle length of germinating rice seeds in the different concentration of DMTU Columns with the different lowercase letters are significantly different at p<0.05.

Fig. 5 Plumule length of germinating rice seeds in the different concentration of DMTU Columns with the different lowercase letters are significantly different at p<0.05.

Fig. 6 Changes of NBT staining in radicle tip of germinated rice seeds treated with different concentrations of DMTU A: 0 mmol/L DMTU; B: 10 mmol/L DMTU; C: 20 mmol/L DMTU; D: 30 mmol/L DMTU; E: 40 mmol/L DMTU; F: 50 mmol/L DMTU; G: 60 mmol/L DMTU.

Fig. 7 Changes of DAB staining in radicle tip of germinated rice seeds treated with different concentrations of DMTU A: 0 mmol/L DMTU; B: 10 mmol/L DMTU; C: 20 mmol/L DMTU; D: 30 mmol/L DMTU; E: 40 mmol/L DMTU; F: 50 mmol/L DMTU; G: 60 mmol/L DMTU.

Fig. 8 Changes of Evans blue staining in root tips of germinated rice seeds treated with different concentrations of DMTU A: 0 mmol/L DMTU; B: 10 mmol/L DMTU; C: 20 mmol/L DMTU; D: 30 mmol/L DMTU; E: 40 mmol/L DMTU; F: 50 mmol/L DMTU; G: 60 mmol/L DMTU.

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Effect of Endogenous H₂O₂ on the Germination of Rice Seeds

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