Biological Characteristics of the Pathogen and Fungicides Screening in Laboratory for Anthracnose of Passiflora edulia Sims

doi:10.3969/j.issn.1000-2561.2021.04.025

Abstract

Abstract:

The experiment was conducted in order to clarify the biological characteristics of Colletotrichum karstii and screen fungicides with better prevention and control. The mycelial growth rate method was applied to explore the biological characteristics of the pathogen and the laboratory toxicity of 9 fungicides to the pathogen. Results indicated that the pathogen was suitable for growth on the SDAY medium with the optimum pH of 6 to 9 and the suitable growth temperature of 20 to 30 ℃, preferably 25 ℃. Besides, it could efficiently utilize sucrose and glycine. Laboratory toxicity test revealed that 9 fungicides had a certain inhibitory effect on the growth of the mycelium of the pathogen. Among them, 75% trifloxystrobin·tebuconazole WG was the most effective with an EC₅₀ of 0.0667 mg/L, followed by procyclidine·azoxystrobin 18.7% SE, fluxapyroxad·pyraclostrobin 42.4% SC, pyraclostrobin 25% SC, prochloraz-manganese chloride complex 50% WP with an EC₅₀ of 0.3335, 0.4567, 0.4630, 0.8187 mg/L, respectively.

Key words: Passiflora edulia Sims, Colletotrichum karstii, biological characteristics, toxicity tests

CLC Number:

S436.67

RAN Fei, CHEN Jia, MO Feixu, FAN Rong, YIN Xianhui, LONG Youhua, WU Xiaomao. Biological Characteristics of the Pathogen and Fungicides Screening in Laboratory for Anthracnose of Passiflora edulia Sims[J]. Chinese Journal of Tropical Crops, 2021, 42(4): 1080-1085.

Figures/Tables 8

Tab. 1 Test fungicides and treatment concentrations

编号 No.	药剂名称 Fungicide	生产厂家 Manufacturers	处理浓度Concentration/(mg·L^-1)
编号 No.	药剂名称 Fungicide	生产厂家 Manufacturers	T₁	T₂	T₃	T₄	T₅
1	17%苯甲·氟酰胺SC	巴斯夫欧洲公司	1.06	2.13	4.25	8.50	17.00
2	75%肟菌·戊唑醇WG	德国拜耳作物科学公司	0.0054	0.022	0.087	0.35	1.39
3	35%氟菌·戊唑醇SC	德国拜耳作物科学公司	0.43	1.30	3.89	11.67	35.00
4	10%苯醚甲环唑WG	先正达南通作物保护有限公司	0.12	0.37	1.11	3.33	10.00
5	18.7%丙环·醚菌酯SC	先正达南通作物保护有限公司	0.08	0.23	0.69	2.08	6.23
6	42.4%唑醚·氟酰胺SC	巴斯夫欧洲公司	0.06	0.17	0.52	1.57	4.17
7	25%丙环唑EC	河南世诚生物科技有限公司	3.09	9.26	27.78	83.33	250.00
8	50%咪鲜胺锰盐WP	江苏辉丰生物农业股份有限公司	0.15	0.46	1.39	4.17	12.50
9	25%吡唑醚菌酯SC	山东康乔生物科技有限公司	0.08	0.23	0.69	2.08	6.25

Fig. 1 Effects of different temperature on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Fig. 2 Effects of different pH on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Fig. 3 Effects of different light condition on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Fig. 4 Effects of different carbon source on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Fig. 5 Effects of different nitrogen source on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Fig. 6 Effects of different medium on mycelial growth of C. karstii Different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01).

Tab. 2 Indoor toxicity test results of 9 fungicides to C. karstii on passion fruit

药剂名称 Fungicide	毒力回归方程 Regression equation of toxicity	EC₅₀ /(mg·L^-1)	相关系数r Correlation coefficient r
75%肟菌·戊唑醇WG	y=5.6235+0.5302x	0.0667	0.9922
18.7%丙环·嘧菌酯SE	y=5.5222+1.0949x	0.3335	0.9941
42.4%唑醚·氟酰胺SC	y=5.3781+1.1109x	0.4567	0.9925
25%吡唑醚菌酯SC	y=5.3522+1.0534x	0.4630	0.9843
50%咪鲜胺锰盐WP	y=5.0861+0.9908x	0.8187	0.9989
35%氟菌·戊唑醇SC	y=4.9716+0.7840x	1.0870	0.9817
10%苯醚甲环唑WG	y=4.7522+0.8257x	1.9960	0.9980
17%苯甲·氟酰胺SC	y=4.6025+0.9734x	2.5607	0.9990
25%丙环唑EC	y=3.0814+1.0488x	67.5018	0.9817

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