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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1700-1707.DOI: 10.3969/j.issn.1000-2561.2020.08.026

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Screening of Antioxidant Sites of Dendrobium aurantiacum Rchb. f. var. denneanum (Kerr.) Z. H. Tsi. Leaves Based on Grey Relational Degree Analysis

RUAN Peihua1,DENG Hong1,2,*(),FU Yongmei3,ZHANG Shu1,2,GUO Cai’e4,HUANG Shubin1,LI Zhichao1   

  1. 1. Key Laboratory of New Drug Dosage Form of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
    2. Guangdong Provincial Precision Medicine Drug Delivery Preparation Engineering Technology Research Center of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
    3. Sinopharm Group Guangdong Global Pharmaceutical Co., Ltd, Foshan, Guangdong 528000, China
    4. Qingyuan Traditional Chinese Medicine Hospital / Guangdong Fresh Medicine and Ethnic Medicine Engineering Technology Research Center, Qingyuan, Guangdong 511500, China.
  • Received:2019-11-06 Revised:2019-11-22 Online:2020-08-25 Published:2020-09-17
  • Contact: DENG Hong

Abstract:

The leaves of Dendrobium aurantiacum Rchb. var. f. denneanum (Kerr.) Z. H. Tsi. were coarse-extracted with different concentrations of ethanol. And then with different polar solvents, the petroleum ether layer, dichloromethane layer, ethyl acetate layer, n-butanol layer and water layer were obtained from 60% ethanol crude extract, whose antioxidant activity was higher. Those layer obtained were assayed with the ultra performance liquid chromatography (UPLC) and UV-spectrophotometric method. With DPPH antioxidant activity as an index, the grey correlation degree correlation analysis was used to screen the main antioxidant activity. The highest amount of flavonoids, 24.89%, was in the n-butyl alcohol layer, and the highest amount of polysaccharide, 0.23%, layer was in the water layer. Each extraction layer had antioxidant activity, and the free radical clearance ability in order from strong to weak was water layer, n-butanol layer, ethyl acetate layer, dichloromethane layer, petroleum ether layer. Besides, the n-butanol layer and water layer were selected as the main antioxidant active parts. And the antioxidant activity was the result of the synergistic action of flavonoids and polysaccharides, which could provide some guidance for further extraction of antioxidant active compounds and development of natural antioxidants.

Key words: leaves of Dendrobium aurantiacum Rchb. var. f. denneanum (Kerr.) Z. H. Tsi., gray relational degree, antioxidant active site, total flavonoids, polysaccharide

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