Identification and Etiology of a New Dangerous <i>Neopestalotiopsis</i> Leaf Fall Disease of Rubber Tree (<i>Hevea brasiliensis</i>) in China
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1616-1624.DOI: 10.3969/j.issn.1000-2561.2020.08.016

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Identification and Etiology of a New Dangerous Neopestalotiopsis Leaf Fall Disease of Rubber Tree (Hevea brasiliensis) in China

LI Boxun,LIU Xianbao,SHI Tao,CAI Jimiao,ZHENG Xiaolan,FENG Yanli,XU Chunhua,HUANG Guixiu()   

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2020-07-23 Revised:2020-07-31 Online:2020-08-25 Published:2020-09-17
  • Contact: HUANG Guixiu


Based on the current outbreak and epidemic situation of a novel Neopestalotiopsis leaf fall disease of rubber trees (Hevea brasiliensis) in major rubber planting countries abroad and its great impact on natural rubber industry economy, the authors conducted a preliminary inspection on 13 farms and rubber plantations in main rubber producing areas in Hainan, Yunnan and Guangdong provinces, and found suspected disease samples in Baoxian Farm, Zhizhong Town, Ledong County, Hainan. In this study, the pathogens of the suspected disease samples were isolated, purified and the pathogenicity was determined. Through morphological and molecular phylogenetic analysis, the pathogen was identified as Neopestalotiopsis aotearoa Maharachch., K.D. Hyde & Crous. The basic biological characteristics showed that the suitable temperature for the mycelial growth and spore germination was 28℃, the lethal temperature of spores was 50℃. The optimum pH value was 6.5, the optimum carbon sources were sucrose and D-glucose, the optimum nitrogen sources were potassium nitrate and soybean peptone, and the optimum medium was PDA, which could produce spores after eight days. The pathogen and its basic biological characteristics were preliminarily identified, which would provide theoretical basis for the reserve of related technical measures for the prevention and control of the disease in advance.

Key words: Hevea brasiliensis, Neopestalotiopsis leaf fall disease, pathogen identification, biological characteristics

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