Physiological and Biochemical Analysis of Oncidium in Response to Stress of Soft Rot Pathogen

doi:10.3969/j.issn.1000-2561.2020.08.015

Abstract

Abstract:

The pseudobulb of Sherry Baby, Oncidium was infected by bacterial soft rot bacteria infection. The first leaf on the pseudobulb was sampled 0, 8, 16, 24, 32 h, after infection to determine the chlorophyll fluorescence properties [F_v/F_m, Y(Ⅱ), Y(NPQ), Y(NO)], superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO) activity, malondialdehyde (MDA), lignin, and the change of soluble sugar content. The results showed that with the extension of infection time, F_v/F_m, Y(Ⅱ), Y(NPQ) gradually decline, Y(NO) increased, and the photosynthetic system was damaged. PPO activity and lignin content decreased gradually; SOD and POD activity increased gradually; CAT activity and soluble sugar content first increased and then decreased; MDA content decreased gradually from 0 to 24 h and then leveled off after 24 h. The study would heip to explore the resistance mechanism and establish the physiological method of resistance identification.

Key words: Oncidium spp., soft rot, physiological and biochemical indexes, chlorophyll fluorescence properties, lignin

CLC Number:

S682.31

WU Qiuzhen,LIN Zhengchun,NI Shanshan,LAI Zhongxiong. Physiological and Biochemical Analysis of Oncidium in Response to Stress of Soft Rot Pathogen[J]. Chinese Journal of Tropical Crops, 2020, 41(8): 1610-1615.

Figures/Tables 10

Fig. 1 Pathophysiology of pseudobulb infected by soft rot pathogen at different time

Fig. 2 Effects of soft rot on Fv/Fm and Y(Ⅱ) of Oncidium

Fig. 3 Effects of soft rot on Y(NPQ) and Y(NO) of Oncidium

Fig. 4 Effects of soft rot on SOD activity in leaves of Oncidium

Fig. 5 Effects of soft rot on CAT activity in leaves of Oncidium

Fig. 6 Effects of soft rot on POD activity in leaves of Oncidium

Fig. 7 Effects of soft rot on PPO activity in leaves of Oncidium

Fig.8 Effects of soft rot on MDA content in leaves of Oncidium

Fig. 9 Effects of soft rot on lignin content in leaves of Oncidium

Fig. 10 Effects of soft rot on soluble sugar content in leaves of Oncidium

References 28

[1]	钟士传. 蝴蝶兰软腐病防治试验[J]. 特产研究, 2005(3):13-14.
[2]	辛海云. 文心兰栽培管理技术[J]. 中国园艺文摘, 2010,26(10):130-131.
[3]	张耀伟. 大白菜抗软腐病生理生化指标研究[D]. 哈尔滨: 东北农业大学, 2002.
[4]	王倩. H₂S延长梨果实及甘薯块根采后贮藏期的抗氧化机制研究[D]. 合肥: 合肥工业大学, 2012.
[5]	秦建彬, 陈俊杰, 魏翠华, 等. 大花蕙兰软腐病防治药剂及防治适期的研究[J]. 农学学报, 2013,3(3):10-12, 38.
[6]	沈会芳, 蒲小明, 张景欣, 等. 蝴蝶兰细菌性软腐病绿色防治药剂筛选试验[J]. 广东农业科学, 2017,44(6):104-108.
[7]	王学亏, 黄见良. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2002: 134-137.
[8]	田韦韦, 王彩霞, 田敏, 等. 文心兰浅绿条纹突变体的生理生化及叶绿素荧光特性研究[J]. 西北植物学报, 2015,35(10):2012-2017.
[9]	任少华, 李媛媛, 邹军, 等. 高温胁迫对四种兰科植物叶绿素荧光参数的影响[J]. 北方园艺, 2017(12):70-74.
[10]	李丹, 黄玉吉, 朱宁, 等. 三明野生蕉抗寒特性的形态学与生理生化研究[J]. 果树学报, 2019,36(1):76-85.
[11]	Zhu S, Ma B. Benzothiadiazole- or methyl jasmonate-induced resistance to Colletotrichum musae in harvested banana fruit is related to elevated defense enzyme activities[J]. Journal of Horticultural Science & Biotechnology, 2007,82(4):500-506.
[12]	李焕改. 百合主要病毒基因组的克隆及感病生理研究[D]. 大连: 大连理工大学, 2010.
[13]	陈亮, 陈年来. 西瓜叶片防御酶活性与枯萎病抗性的关系[J]. 河南农业科学, 2019,48(1):77-83, 114.
[14]	孙正祥, 王丰, 周燚. 内生菌XG-1对西瓜枯萎病诱导抗性的研究[J]. 河南农业科学, 2013,42(3):71-75.
[15]	覃柳燕, 郭成林, 黄素梅. 棘孢木霉菌株PZ6对香蕉促生效应及枯萎病室内防效的影响[J]. 南方农业学报, 2017,48(2):277-283.
[16]	刘学庆, 王秀峰, 朴永吉. 蝴蝶兰不同品种耐冷特性的研究[J]. 园艺学报, 2007,34(2):425-430.
[17]	逯明辉, 宋慧, 李晓明, 等. 冷害过程中黄瓜叶片SOD、CAT和POD活性的变化[J]. 西北植物学报, 2005(8):1570-1573.
[18]	贺立红, 宾金华. 高等植物中的多酚氧化酶[J]. 植物生理学通讯, 2001(4):340-345.
[19]	张晓南, 孙佩佩, 茅云翔, 等. 紫菜腐霉侵染对条斑紫菜叶状体防御性物质含量及保护性酶类活性的影响[J]. 中国海洋大学学报(自然科学版), 2015,45(12):57-64.
[20]	丁玉梅, 马龙海, 周晓罡等. 干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析[J]. 西南农业学报, 2013,26(1):106-110.
[21]	Shishatskaya E, Menzianova N, Zhila N, et al. Toxic effects of the fungicide tebuconazole on the root system of fusarium-infected wheat plants[J]. Plant Physiology and Biochemistry, 2018(132):400-407.
[22]	邢红爽, 张瑞, 郭绍霞. 高温胁迫下丛枝菌根真菌对百合耐热性的影响[J]. 青岛农业大学学报(自然科学版), 2018,35(4):258-264.
[23]	徐叶挺, 李疆, 罗淑萍, 等. 低温胁迫下野生巴旦杏抗寒生理指标的测定[J]. 新疆农业大学学报, 2008,31(4):1-4.
[24]	高长敏, 马光恕, 廉华, 等. 木霉菌对黄瓜幼苗生长和膜脂过氧化指标的影响及对枯萎病的防治效果[J]. 中国生物防治学报, 2018,34(5):762-770.
[25]	迟建国. 白腐菌对烟叶木质素含量的影响[J]. 贵州农业科学, 2013,41(7):138-139.
[26]	王乾, 杨亮, 胡艳敏, 等. 5个构树地方品种耐寒性状研究[J]. 北京农学院学报, 2019,34(2):94-96.
[27]	李赤, 于莉, 刘付东标, 等. 富贵竹中可溶性糖、蛋白质含量与细菌性茎腐病的关系[J]. 吉林农业大学学报, 2007(6):620-622.
[28]	魏崃, 王伟威, 李馨园, 等. 大豆抗腐霉根腐病的生理差异研究[J]. 大豆科学, 2017,36(3):425-429.