Identification and Domestication Cultivation of a Wild-type Ganoderma lingzhi Strain from Lincang, Yunnan

doi:10.3969/j.issn.1000-2561.2020.11.005

Abstract

Abstract:

An optimal medium was screened for the cultivation of a wild-type Ganoderma lingzhi, which are the basis of its mass production. In this paper, a strain of YAASM4672 was isolated from a wild mushroom fruiting body of Ganoderma lingzhi, from Lincang, Yunnan. Taxonomic status was identified based on its morphology and ITS sequence analysis, and nine media of culture spawn were designed for the determination of its cultivation characteristics. The result showed that the wild strain of YAASM4672 was G. lingzhi, and the morphological characteristics were consistent with its molecular identification. Formula 2 of culture spawn were the best for the mycelial growth and agronomic characters of fruiting body (soil mulched group was better than the open field group) of strain YAASM4672. Its mycelia colour was white and dense, the time of sackful was 38 d, and the growth rate of mycelia was (5.89±1.01) mm/d. The pileus was yellow, diameter was 66.00 mm, and average growth rate was (4.14±1.07) mm/d, which were significantly (P<0.05) or extremely significantly (P<0.01) different compared with other formula. Stipe was brownish yellow, its length and diameter was 84.00 mm and 18.00 mm respectively. In short, the optimal medium for artificial cultivation of strain YAASM4672 was 78% corn cob, 18% rice bran, 2% sorghum flour, 1% gypsum powder, and 1% white granulated sugar. Soil mulched can promote the growth and development of its fruiting body.

Key words: Ganoderma lingzhi, morphology, ITS sequencing analysis, domestication cultivation

CLC Number:

S567.3+1

ZHANG Yanzhen,ZHOU Huiming,BAI Yuying,CHAI Hongmei,GU Guanghong,ZHAO Yatong,TIAN Libo,MAO Mingjuan. Identification and Domestication Cultivation of a Wild-type Ganoderma lingzhi Strain from Lincang, Yunnan[J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2176-2182.

Figures/Tables 9

References 31

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配方 Formulae	满袋时间 Sackful time/d	菌丝生长势 Mycelial growth vigour	菌丝颜色 Mycelial colour	菌丝生长速度 Mycelial growth rate/(mm?d^-1)
1	41	+++	白色	5.10±0.27^bcAB
2	38	+++	白色	5.89±1.01^aA
3	50	+++	淡黄色	3.58±0.72^efCD
4	64	++	白色	2.24±0.23^gF
5	39	+++	白色	5.42±1.26^abA
6	42	+++	白黄色	4.39±1.02^cdBC
7	53	+++	微黄色	3.02±0.34^fEF
8	43	+++	白色	4.10±1.09^deBC
9	44	++	白色	4.01±1.22^deCD

配方 Formulae	满袋时间 Sackful time/d	菌丝生长势 Mycelial growth vigour	菌丝颜色 Mycelial colour	菌丝生长速度 Mycelial growth rate/(mm?d^-1)
1	41	+++	白色	5.10±0.27^bcAB
2	38	+++	白色	5.89±1.01^aA
3	50	+++	淡黄色	3.58±0.72^efCD
4	64	++	白色	2.24±0.23^gF
5	39	+++	白色	5.42±1.26^abA
6	42	+++	白黄色	4.39±1.02^cdBC
7	53	+++	微黄色	3.02±0.34^fEF
8	43	+++	白色	4.10±1.09^deBC
9	44	++	白色	4.01±1.22^deCD

配方 Formulae	菌盖Pileus			菌柄Stipe
配方 Formulae	生长速度 Growth rate/(mm?d^-1)	直径 Diameter/mm	颜色 Colour	长度 Length/mm	直径 Diameter/mm	颜色 Colour
1	—	—	—	—	—	—
2	3.01±0.17^aA	51.00	金橘黄或金黄	65.50	17.00	褐红
3	2.34±0.91^abA	15.00	淡黄	65.00	16.00	棕红
4	—	—	—	—	—	—
5	2.02±0.88^bA	25.20	白里透黄	80.00	12.00	褐黄
6	1.69±0.11^bA	16.00	白里透黄	40.00	7.00	褐黄
7	—	—	—	—	—	—
8	—	—	—	—	—	—
9	—	—	—	—	—	—

配方 Formulae	菌盖Pileus			菌柄Stipe
配方 Formulae	生长速度 Growth rate/(mm?d^-1)	直径 Diameter/mm	颜色 Colour	长度 Length/mm	直径 Diameter/mm	颜色 Colour
1	—	—	—	—	—	—
2	3.01±0.17^aA	51.00	金橘黄或金黄	65.50	17.00	褐红
3	2.34±0.91^abA	15.00	淡黄	65.00	16.00	棕红
4	—	—	—	—	—	—
5	2.02±0.88^bA	25.20	白里透黄	80.00	12.00	褐黄
6	1.69±0.11^bA	16.00	白里透黄	40.00	7.00	褐黄
7	—	—	—	—	—	—
8	—	—	—	—	—	—
9	—	—	—	—	—	—

配方 Formulae	菌盖Pileus			菌柄Stipe
配方 Formulae	生长速度 Growth rate/(mm?d^-1)	直径 Diameter/mm	颜色 Colour	长度 Length/mm	直径 Diameter/mm	颜色 Colour
1	—	—	—	—	—	—
2	4.14±1.07^aA	66.00	黄色	84.00	18.00	褐黄
3	3.00±0.83^cB	43.00	边缘白里透黄,其余黄色	30.00	12.00	褐黄
4	—	—	—	—	—	—
5	2.39±1.01^dC	25.00	白里透黄	23.00	15.00	褐黄
6	2.46±0.55^dBC	28.00	边缘白色,其余黄色	30.00	16.00	褐黄
7	1.42±0.72^eD	35.00	棕色	29.00	17.00	褐土黄
8	3.59±1.07^bA	45.00	土黄色	25.00	12.00	褐黄
9	—	—	—	—	—	—