Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2176-2182.DOI: 10.3969/j.issn.1000-2561.2020.11.005

• Germplasm Resources, Genetics & Breeding • Previous Articles     Next Articles

Identification and Domestication Cultivation of a Wild-type Ganoderma lingzhi Strain from Lincang, Yunnan

ZHANG Yanzhen1,2,ZHOU Huiming1,2,BAI Yuying1,CHAI Hongmei2,*(),GU Guanghong1,ZHAO Yatong1,TIAN Libo1,MAO Mingjuan1   

  1. 1. School of Biotechnology and Engineering, West Yunnan University, Lincang, Yunnan 677000, China
    2. Institute of Biotechnology and Germplasmic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650221, China
  • Received:2019-11-28 Revised:2019-12-27 Online:2020-11-25 Published:2020-12-23
  • Contact: CHAI Hongmei


An optimal medium was screened for the cultivation of a wild-type Ganoderma lingzhi, which are the basis of its mass production. In this paper, a strain of YAASM4672 was isolated from a wild mushroom fruiting body of Ganoderma lingzhi, from Lincang, Yunnan. Taxonomic status was identified based on its morphology and ITS sequence analysis, and nine media of culture spawn were designed for the determination of its cultivation characteristics. The result showed that the wild strain of YAASM4672 was G. lingzhi, and the morphological characteristics were consistent with its molecular identification. Formula 2 of culture spawn were the best for the mycelial growth and agronomic characters of fruiting body (soil mulched group was better than the open field group) of strain YAASM4672. Its mycelia colour was white and dense, the time of sackful was 38 d, and the growth rate of mycelia was (5.89±1.01) mm/d. The pileus was yellow, diameter was 66.00 mm, and average growth rate was (4.14±1.07) mm/d, which were significantly (P<0.05) or extremely significantly (P<0.01) different compared with other formula. Stipe was brownish yellow, its length and diameter was 84.00 mm and 18.00 mm respectively. In short, the optimal medium for artificial cultivation of strain YAASM4672 was 78% corn cob, 18% rice bran, 2% sorghum flour, 1% gypsum powder, and 1% white granulated sugar. Soil mulched can promote the growth and development of its fruiting body.

Key words: Ganoderma lingzhi, morphology, ITS sequencing analysis, domestication cultivation

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