Ploidy Induction Effect of Dinitroaniline Herbicides on the Single-headed Cutting <i>Chrysanthemum</i> ‘Iwa-no-hakusen’
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (11): 2190-2196.DOI: 10.3969/j.issn.1000-2561.2020.11.007

• Germplasm Resources, Genetics & Breeding • Previous Articles     Next Articles

Ploidy Induction Effect of Dinitroaniline Herbicides on the Single-headed Cutting Chrysanthemum ‘Iwa-no-hakusen’

YANG Huiting1,CHEN Guixin1,HU Jihong1,JI Yuting1,KANG Jianban2,PAN Tengfei1,SHE Wenqin1,*()   

  1. 1. School of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Pingnan County United Front Work Department, Pingnan, Fujian 352301, China
  • Received:2019-08-05 Revised:2020-01-31 Online:2020-11-25 Published:2020-12-23
  • Contact: SHE Wenqin

Abstract:

In this study, the stalk bud stems of Japanese single-headed cutting Chrysanthemum ‘Iwa-no-hakusen’ sterile seedlings were used as the materials, and two dinitroaniline herbicides (pendimethalin and trifluralin) were used at different concentrations and soaking time. Induction treatment was carried out and inoculated into rooting medium to induce whole plants. The ploidy inducing effect of herbicide on the sterile stem segment of cutting Chrysanthemum ‘Iwa-no-hakusen’ was summarized by statistical analysis and comparison of early mutant morphology, chromosome count of root tip cells, leaf stomatal characteristics, agronomic traits and flowering morphology. The results showed that the induction effect was the best when induced by 200 μmol/L pendimethalin and 200 μmol/L trifluralin for 36 h and 24 h, the induction rate was 25.00% and 40.00%. The number of chromosomes in the plants increased, and the agronomic traits changed significantly. The leaf guard cells and stomata of the mutant plants increased, the stomatal density decreased, the plant height and the internode length were significantly shortened, the stem diameter, leaf length and leaf width increased significantly, and the shape of the tongue petals showed various changes and the number increased.

Key words: cutting Chrysanthemum, chemical mutagenesis, dinitroaniline, aneuploid, Iwa-no-hakusen

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