Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 929-938.DOI: 10.3969/j.issn.1000-2561.2020.05.012

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Genetic Diversity Analysis of Cymbidium Germplasms Based on SRAP Markers

YUAN Yuan1,CAO Bin1,ZHANG Yongqi1,CHEN Qingxi1,*(),CHEN Nanchuan2   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Fujian Bainong Ecological Technology Co., Ltd, Zhao’an, Fujian 363500, China
  • Received:2019-08-05 Revised:2019-08-26 Online:2020-05-25 Published:2020-06-15
  • Contact: CHEN Qingxi

Abstract:

SRAP (sequence related amplified polymorphism) was applied for the determination of the diversity of 154 samples of Cymbidium germplasm. 16 SRAP primer pairs were screened from 168 SRAP primers and applied to all the materials, resulting in 874 amplified DNA bands. For the amplified DNA bands, 857 were polymorphic, with a ratio of 98.1%. The average number of bands of each SRAP primer for each Cymbidium germplasm was 5.74. UPGMA cluster analysis showed that the genetic diversity and genetic relationship of the Cymbidium germplasms could be revealed by Me6-Em3 primer pair and could be classified into eight groups with a similarity coefficient 0.818 and the genetic similarity of the germplasms ranged from 0.772 to 1.000. It was found for the first time that the primer pairs Me8-Em4, Me11-Em2 and Me12-Em11 could be used together to identify C. Ensifolium reliably. Results in the present study could provide references for the utilization of Cymbidium germplasm and the identification of Cymbidium hybrid cultivars.

Key words: Chinese Cymbidium, germplasm, SRAP marker, genetic diversity

CLC Number: