Morphological Development of Primary Laticifers in Shoots of <i>Hevea brasiliensis</i> Muell. Arg
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 881-887.DOI: 10.3969/j.issn.1000-2561.2020.05.006

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Morphological Development of Primary Laticifers in Shoots of Hevea brasiliensis Muell. Arg

TAN Deguan1,Anuwat KUMPEANGKEAW1,2,HAN Bingying1,FU Lili1,SUN Xuepiao1,ZHANG Jiaming1,*()   

  1. 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS) / Hainan Academy of Tropical Agricultural Resource, CATAS / Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    2. Song Khla Rubber Research Centre, Department of Agriculture, Ministry of Agriculture and Cooperatives, Had Yai, Song Khla 90110, Thailand
  • Received:2019-07-09 Revised:2019-08-20 Online:2020-05-25 Published:2020-06-15
  • Contact: ZHANG Jiaming


The primary laticifers of Hevea brasiliensis Muell. Arg present mainly in young trunks, shoots, leaves, flowers, and fruits. However, its morphological development is unknown. Two methods, parafin section and modified alkali-treated stripping were used to study the development of the primary laticifers in this study, and the latter was found superior to the former by continuous observation of the whole developmental process. The morphological development of the primary laticifers of different stages was systematically investigated using the modified alkali-treated stripping method. The results showed that the laticifers isolated from different positions of the bronze-stage shoots were morphologically distinct. The laticifers close to the apical meristem were longtube-like linear structures without end-walls and branches, and protrusive growth was not obvious. The lacifers at 7 cm away from the apical meristem had obvious intrusive growth and bead-like structures, which resulting in increase in the diameter of laticifers. The laticifers at 14 cm away from the apical meristem were significantly enlarged in diameter, and contained more bead-like structures, and the bead-like structures between the neighboring laticifers were connected to form laticifer bridges, which resulting in the formation of a laticifer network. The laticifers isolated from different positions of the stable-stage shoots were morphologically similar, and the density of the protrusions or bead-like structures and the laticifer bridges was significantly higher than that in bronze-stage shoots, and complex laticifer networks were formed in the barks at all positions. The primary laticifers were proved to be multinuclear as well. The results of this study indicate that the primary laticifers in the rubber tree shoots are non-articulated and anastomosing, and belong to a novel type of laticifers. This study would play an important role in the fundamental research of the plant laticifer development.

Key words: Hevea brasiliensis Muell. Arg, primary laticifer, novel type of laticifer, non-articulated and anastomosing laticifer

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