Comparative Analysis of Active Ingredients of Different Collection Times from Different Geographical Sources in Anoectochilus roxburghii and Anoectochilus formosanus

doi:10.3969/j.issn.1000-2561.2020.04.024

Abstract

Abstract:

The biomass and active ingredients of four cultivars (‘Sanming NL (narrow leaf)’, ‘Sanming RL (round leaf)’, ‘Zhangzhou NL’ and ‘Taiwan AF’) of Anoectochilus roxburghii and Anoectochilus formosanus from different geographical sources were assayed 120 days after transplantation. The polysaccharides content, fresh weight and dry weight per plant of ‘Zhangzhou NL’ were significantly higher than that of the other three cultivars. Also, the total polyphenols and total flavonoids content of ‘Taiwan AF’ were significantly higher than that of the other three cultivars. The dynamic changes of the active ingredients in different collection periods were investigated. Except for ‘Taiwan AF’ cultivar, the variation rules of total polyphenols, total flavonoids and polysaccharides content in the three varieties were roughly the same. The content of total polyphenols and total flavonoids increased and the content of polysaccharides decreased with the extension of transplanting time. The content of total polyphenols and total flavonoids reached the maximum 150 days after transplantation for ‘Sanming NL’ and ‘Sanming RL’. For ‘Sanming NL’, the content of total polyphenols and total flavonoids was (9.81±0.24) and (4.62±0.16) mg/g, respectively. For ‘Sanming RL’, the content of total polyphenols and total flavonoids were (9.27±0.22) and (4.60±0.17) mg/g, respectively. In ‘Zhangzhou RL’, the content of total polyphenols and total flavonoids reached the maximum 120 days after transplantation. The content of total polyphenols and total flavonoids in ‘Zhangzhou RL’ was (7.74±0.05) and (4.63±0.04) mg/g, respectively. The content of polysaccharides reached the maximum 90, 0 and 120 days after transplantation for ‘Sanming NL’, ‘Sanming RL’ and ‘Zhangzhou RL’, respectively. The content of polysaccharides was (26.05±2.11), (24.29±2.33) and (45.90±4.70) mg/g in ‘Sanming NL’, ‘Sanming RL’ and ‘Zhangzhou RL’, respectively. Besides, the analysis of the active components in different parts in the plant of ‘Sanming NL’ and ‘Sanming RL’ revealed that polyphenols and flavonoids were mainly accumulated in the leaves but sugars in the stems.

Key words: Anoectochilus roxburghii, collection time, biological characteristics, active ingredients

CLC Number:

R282.4

ZENG Biyu,SU Minghua,CHEN Qingxi,LI Huihua,CHANG Qiang,WANG Wei. Comparative Analysis of Active Ingredients of Different Collection Times from Different Geographical Sources in Anoectochilus roxburghii and Anoectochilus formosanus[J]. Chinese Journal of Tropical Crops, 2020, 41(4): 811-818.

Figures/Tables 8

References 15

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品种 Cultivars	单株鲜重 Fresh weigh per plant	单株干重 Dry weigh per plant
三明尖叶	638±91^c	51±9^c
三明圆叶	756±83^c	67±9^b
漳州尖叶	1496±375^a	138±36^a
台湾AF	920±113^b	61±8^bc

品种 Cultivars	单株鲜重 Fresh weigh per plant	单株干重 Dry weigh per plant
三明尖叶	638±91^c	51±9^c
三明圆叶	756±83^c	67±9^b
漳州尖叶	1496±375^a	138±36^a
台湾AF	920±113^b	61±8^bc

品种 Cultivars	总多酚 Total polyphenols	总黄酮 Total flavonoids	多糖 Polysaccharides
三明尖叶	8.11±0.22^c	3.62±0.18^c	12.85±1.72^b
三明圆叶	8.96±0.09^b	3.86±0.07^b	9.41±0.76^b
漳州尖叶	6.60±0.12^d	3.14±0.15^d	45.90±2.71^a
台湾AF	9.33±0.18^a	4.55±0.06^a	9.61±0.35^b

品种 Cultivars	总多酚 Total polyphenols	总黄酮 Total flavonoids	多糖 Polysaccharides
三明尖叶	8.11±0.22^c	3.62±0.18^c	12.85±1.72^b
三明圆叶	8.96±0.09^b	3.86±0.07^b	9.41±0.76^b
漳州尖叶	6.60±0.12^d	3.14±0.15^d	45.90±2.71^a
台湾AF	9.33±0.18^a	4.55±0.06^a	9.61±0.35^b

天数Days	三明尖叶 Sanming NL	三明圆叶 Sanming RL	漳州尖叶 Zhangzhou NL	台湾AF Taiwan AF
0	813±146^a	577±100^c	1115±172^c	662±105^d
30	755±144^b	577±86^c	1232±135^c	880±167^c
60	619±103^b	644±115^c	1314±258^bc	942±153^c
90	831±190^a	727±187^ab	1124±222^c	975±190^c
120	638±91^b	756±83^ab	1496±375^b	920±113^c
150	845±106^a	655±103^bc	1512±245^b	1102±184^b
180	841±218^a	826±267^a	1705±334^a	1285±213^a