Dissipation, Residues and Dietary Risk Assessment of Difenoconazole and Thifluzamide in Banana

doi:10.3969/j.issn.1000-2561.2020.03.024

Abstract

Abstract:

A supervised residue trial was carried out in Yunnan in 2016 to study the digestion process of difenoconazole and thifluzamide in banana fruit, banana flesh and soil to analyze the residual digestion behavior and dietary intake risk in banana. The risk of long-term dietary exposure in China was assessed. The results showed that the linear equation of the standard curve of difenoconazole was y=1.45×10 ⁷x+4.14×10 ⁴, R ²=0.9962, and that of thifluzamide was y=2.99× 10 ⁶x+1.43×10 ⁴, R ²=0.9954. At the level of 0.04-1 mg/kg, the average recovery of difenoconazole was 75%-110%, and the relative standard deviation (RSD) was 0.9%-6.2%, while the average recovery of thifluzamide was 76%-114%, and RSD was 3.0%-9.5%. The limit of quantification (LOQ) of difenoconazole and thifluzamide in soil, banana fruit and banana flesh was 0.04 mg/kg, and the limit of detection (LOD) was 5 pg. The digestion of difenoconazole and thifluzamide was basically in accordance with the first-order kinetic equation. The half-life of difenoconazole in banana fruit and soil was 16 and 20 days, and the half-life of thifluzamide in banana fruit and soil was 20 and 27 days. Risk assessments indicated that the long-term dietary intake risk of difenoconazole in banana was acceptable, while thifluzamide had a certain long-term dietary risk.

Key words: banana, difenoconazole, thifluzamide, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), residue and dissipation, risk assessment

CLC Number:

X839.2

ZHOU Min,HE Xiufen,DONG Cunzhu,GE Huilin. Dissipation, Residues and Dietary Risk Assessment of Difenoconazole and Thifluzamide in Banana[J]. Chinese Journal of Tropical Crops, 2020, 41(3): 596-602.

Figures/Tables 5

References 19

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农药 Pesticide	基质 Matrix	添加水平 Spiked level/(mg∙kg^-1)	回收率 Recovery/%						相对标准偏差RSD/%
农药 Pesticide	基质 Matrix	添加水平 Spiked level/(mg∙kg^-1)	1	2	3	4	5	平均回收率 Average recovery/%	相对标准偏差RSD/%
苯醚甲环唑	全蕉	1.00	90	84	82	81	77	83	5.6
		0.10	90	88	92	91	98	92	4.0
		0.04	95	85	86	83	94	88	6.2
	蕉肉	1.00	88	86	86	84	90	87	2.6
		0.10	93	90	84	95	92	91	4.6
		0.04	90	79	88	82	82	84	5.4
	土壤	1.00	76	71	76	77	77	75	3.1
		0.10	98	98	100	99	99	99	0.9
		0.04	111	120	108	105	105	110	5.6
噻呋酰胺	全蕉	1.00	74	72	80	75	79	76	4.4
		0.10	106	103	98	103	92	100	5.4
		0.04	110	112	89	111	101	104	9.5
	蕉肉	1.00	81	81	86	88	86	84	3.7
		0.10	115	111	110	124	109	114	5.4
		0.04	105	101	85	100	100	98	8.0
	土壤	1.00	78	83	78	76	77	78	3.6
		0.10	100	90	99	109	98	99	6.7
		0.04	104	102	105	102	90	101	6.0

农药 Pesticide	基质 Matrix	添加水平 Spiked level/(mg∙kg^-1)	回收率 Recovery/%						相对标准偏差RSD/%
农药 Pesticide	基质 Matrix	添加水平 Spiked level/(mg∙kg^-1)	1	2	3	4	5	平均回收率 Average recovery/%	相对标准偏差RSD/%
苯醚甲环唑	全蕉	1.00	90	84	82	81	77	83	5.6
		0.10	90	88	92	91	98	92	4.0
		0.04	95	85	86	83	94	88	6.2
	蕉肉	1.00	88	86	86	84	90	87	2.6
		0.10	93	90	84	95	92	91	4.6
		0.04	90	79	88	82	82	84	5.4
	土壤	1.00	76	71	76	77	77	75	3.1
		0.10	98	98	100	99	99	99	0.9
		0.04	111	120	108	105	105	110	5.6
噻呋酰胺	全蕉	1.00	74	72	80	75	79	76	4.4
		0.10	106	103	98	103	92	100	5.4
		0.04	110	112	89	111	101	104	9.5
	蕉肉	1.00	81	81	86	88	86	84	3.7
		0.10	115	111	110	124	109	114	5.4
		0.04	105	101	85	100	100	98	8.0
	土壤	1.00	78	83	78	76	77	78	3.6
		0.10	100	90	99	109	98	99	6.7
		0.04	104	102	105	102	90	101	6.0

农药Pesticide	基质Matrix	C₀/(mg∙kg^-1)	K	R²	DT₅₀/d	消解方程Digestion equation
苯醚甲环唑	土壤	3.1	0.035	0.909	20	C=3.1×exp(-0.035t)
	全蕉	1.3	0.044	0.961	16	C=1.3×exp(-0.044t)
噻呋酰胺	土壤	3.0	0.026	0.720	27	C=3.0×exp(-0.026t)
	全蕉	1.8	0.035	0.964	20	C=1.8×exp(-0.035t)

农药Pesticide	基质Matrix	C₀/(mg∙kg^-1)	K	R²	DT₅₀/d	消解方程Digestion equation
苯醚甲环唑	土壤	3.1	0.035	0.909	20	C=3.1×exp(-0.035t)
	全蕉	1.3	0.044	0.961	16	C=1.3×exp(-0.044t)
噻呋酰胺	土壤	3.0	0.026	0.720	27	C=3.0×exp(-0.026t)
	全蕉	1.8	0.035	0.964	20	C=1.8×exp(-0.035t)

基质Matrix	施药剂量Application dosage/(mg∙kg^-1)	施药次数 Application frequency	噻呋酰胺残留量 Thifluzamide residual/(mg∙kg^-1)			苯醚甲环唑残留量 Difenoconazole residual/(mg∙kg^-1)
			采收间隔期Preharvest interval/d			采收间隔期Preharvest interval/d
			35	42	49	35	42	49
全蕉	417	3	<0.040	<0.040	<0.040	<0.040	<0.040	<0.040
	417	4	0.078	0.068	<0.040	<0.040	<0.040	<0.040
	278	3	0.091	<0.040	<0.040	0.092	<0.040	<0.040
	278	4	0.13	0.18	<0.040	<0.040	<0.040	<0.040
蕉肉	417	3	<0.040	<0.040	<0.040	<0.040	<0.040	<0.040
	417	4	<0.040	<0.040	<0.040	<0.040	<0.040	<0.040
	278	3	<0.040	<0.040	<0.040	<0.040	<0.040	<0.040
	278	4	<0.040	<0.040	<0.040	<0.040	<0.040	<0.040
土壤	417	3	0.062	0.10	0.13	0.077	0.29	0.17
	417	4	0.45	0.42	0.23	0.27	0.27	0.10
	278	3	0.15	0.29	0.30	0.20	0.19	0.16
	278	4	0.14	0.40	0.19	0.17	0.21	0.090