Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 579-585.DOI: 10.3969/j.issn.1000-2561.2020.03.022
• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles Next Articles
ZHU Kexue1,2,DAO Chunli1,3,GU Wenliang1,XU Fei1,TAN Lehe1,2,*()
Received:
2019-05-29
Revised:
2019-08-14
Online:
2020-03-25
Published:
2020-04-16
Contact:
TAN Lehe
CLC Number:
ZHU Kexue,DAO Chunli,GU Wenliang,XU Fei,TAN Lehe. Antioxidant Activities and GC-MS Analysis of Ilex kudingcha C.J. Tseng Extracts by Different Extraction Methods[J]. Chinese Journal of Tropical Crops, 2020, 41(3): 579-585.
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Fig. 1 DPPH• scavenging activities of I. kudingcha C.J. Tseng extracts by different extraction methods Different lowercase letters indicate significant difference (P< 0.05).
Fig. 2 •OH scavenging activities of I. kudingcha C.J. Tseng extracts by different extraction methods Different lowercase letters indicate significant difference (P< 0.05).
Fig. 3 O2-• scavenging activities of I. kudingcha C.J. Tseng extracts by different extraction methods Different lowercase letters indicate significant difference (P<0.05).
Fig. 4 ABTS+• scavenging activities of I. kudingcha C.J. Tseng extracts by different extraction methods Different lowercase letters indicate significant difference (P<0.05).
类别 Types | 保留时间Retention time /min | 化学成分 Chemical components | 超声-乙醇 Ultrasound -ethanol | 超声-乙酸乙酯 Ultrasound-ethyl acetate | 超声-石油醚 Ultrasound- petroleum ether | 超临界CO2 Supercritical CO2 |
---|---|---|---|---|---|---|
烷烃类 | 33.121 | 十六烷(Hexadecane) | - | + | - | - |
33.324 | 十三烷(Tridecane, 1-iodo-) | - | + | - | - | |
39.503 | 二十四烷(Tetracosane) | - | + | - | - | |
41.876 | 癸烷(Decane, 3,6-dimethyl-) | - | + | - | - | |
42.602 | 十五烷(Pentadecane) | + | + | - | - | |
43.311 | 环氧十二烷(13-Oxabicyclo[10.1.0]tridecane) | - | + | + | - | |
44.413 | 二十烷(Eicosane) | - | + | - | - | |
44.907 | 二十六烷(Hexacosane) | - | + | - | - | |
46.122 | 1,2-环氧十六烷(Oxirane, tetradecyl-) | - | + | - | - | |
47.129 | 十八烷(Octadecanal) | + | - | - | + | |
47.588 | 三癸基环氧乙烷(Oxirane, tridecyl-) | - | + | - | - | |
48.076 | 壬烷(9-Oxabicyclo[6.1.0]nonane) | - | + | - | + | |
49.506 | 二十八烷(Octacosane) | - | + | + | + | |
54.140 | 四十四烷(Tetratetracontane) | + | - | - | + | |
55.155 | 1,2-环氧环辛烷(9-Oxabicyclo[6.1.0]nonane, cis-) | - | - | + | - | |
62.616 | 氯代十六烷(Hexadecane, 1-chloro-) | - | + | + | - | |
67.922 | 十九烷(Nonadecane) | - | + | - | - | |
74.300 | 1,2-环氧十六烷(Oxirane, tetradecyl-) | + | - | - | - | |
74.382 | 胆甾烷 [Cholestane, 4,5-epoxy-, (4.alpha.,5.alpha.)-] | - | + | + | + | |
酯类 | 30.161 | 丙氨酸乙酯(D-Alanine, N-ethoxycarbonyl-, ethyl ester) | - | + | - | - |
36.745 | 亚硫酸,十八烷基2-丙酯(Sulfurous acid, octadecyl 2-propyl ester) | - | + | - | + | |
37.103 | 棕榈酸甲酯(Hexadecanoic acid, methyl ester) | + | - | - | + | |
37.689 | 邻苯二甲酸,壬酯(Phthalic acid, nonyl pentadecyl ester) | - | - | - | + | |
38.748 | 2-己基-1-癸醇硫酸氢酯(1-Decanol, 2-hexyl-) | - | + | - | - | |
45.614 | 乙酸正十七烷基酯(Heptadecyl acetate) | + | + | + | + | |
53.151 | 十七碳二烯酸甲酯(Methyl 9,12-heptadecadienoate) | - | - | + | - | |
53.643 | 9,12-十八酸(Z,Z)-, 2-羟基-1-(羟甲基)乙基酯 [9,12-Octadecadienoic acid (Z,Z)-, 2-hydroxy-1- (hydroxymethyl)ethyl ester] | - | - | + | - | |
酸类 | 38.009 | 2-溴十六烷酸(n-Hexadecanoic acid) | + | + | + | + |
48.979 | 油酸(Oleic Acid) | + | + | + | + | |
50.097 | 反式-13-十八碳烯酸(trans-13-Octadecenoic acid) | - | - | + | - | |
54.664 | 顺式-十八碳烯酸(cis-Vaccenic acid) | - | + | - | + | |
53.335 | 十七酸(Heptadecanoic acid, heptadecyl ester) | - | + | - | ||
69.995 | 11,13-二甲基-12-顺醋酸(11,13-Dimethyl-12-tetradecen-1-ol acetate) | + | - | - | + | |
烯烃类 | 35.566 | 新植二烯(Neophytadiene) | + | + | + | + |
51.434 | 6,11-二甲基-2,6,10-十二碳三烯(6,11-Dimethyl-2,6,10-dodecatrien-1-ol) | + | + | + | + | |
59.674 | 17-三十五烯(17-Pentatriacontene) | - | + | - | - | |
63.776 | 1,E-11,Z-13-十八碳三烯(1,E-11,Z-13-Octadecatriene) | - | + | - | + | |
其他类 | 43.974 | 油酸酰胺 [9-Octadecenamide, (Z)-] | - | - | - | + |
5.314 | 氯丙氧基苯 [benzene, 1-phenoxy-3- (4-phenoxyphenoxy)-] | - | + | - | - | |
类别 Types | 保留时间Retention time /min | 化学成分 Chemical components | 超声-乙醇 Ultrasound -ethanol | 超声-乙酸乙酯 Ultrasound-ethyl acetate | 超声-石油醚 Ultrasound- petroleum ether | 超临界CO2 Supercritical CO2 |
其他类 | 39.823 | 2,2-亚甲基双-(4-甲基-6-叔丁基苯酚) Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl- | + | + | - | - |
40.127 | 鲸蜡基戊烷基醚(Hexadecyl pentyl ether) | + | - | + | + | |
51.011 | 十八烷基乙烯基醚 [Octadecane, 1-(ethenyloxy)-] | - | - | + | - | |
40.814 | 聚酰胺树 [9,12-Octadecadienoic acid (Z,Z)-] | + | + | + | + | |
48.471 | 9,17-十八二烯醛 [9,17-Octadecadienal, (Z)-] | + | + | + | + | |
52.119 | 亚麻酰氯 [9,12-Octadecadienoyl chloride, (Z,Z)-] | - | + | + | + | |
54.175 | 12-甲基-反,反-2,13-十八碳二烯-1-醇 (12-Methyl-E,E-2,13-octadecadien-1-ol) | - | + | + | - | |
59.972 | 苯并二氧六环(1,4 Benzodioxan-6-amine) | + | - | + | + | |
61.529 | 正十八烷-5,4-二丁基(Octadecane, 5,14-dibutyl-) | - | + | - | - | |
70.032 | 顺,顺-2-甲基-3,13-十八二烯醇(2-Methyl-Z,Z-3,13-octadecadienol) | - | + | + | - |
Tab. 1 Chemical components of I. kudingcha C. J. Tseng extracts by different extraction methods
类别 Types | 保留时间Retention time /min | 化学成分 Chemical components | 超声-乙醇 Ultrasound -ethanol | 超声-乙酸乙酯 Ultrasound-ethyl acetate | 超声-石油醚 Ultrasound- petroleum ether | 超临界CO2 Supercritical CO2 |
---|---|---|---|---|---|---|
烷烃类 | 33.121 | 十六烷(Hexadecane) | - | + | - | - |
33.324 | 十三烷(Tridecane, 1-iodo-) | - | + | - | - | |
39.503 | 二十四烷(Tetracosane) | - | + | - | - | |
41.876 | 癸烷(Decane, 3,6-dimethyl-) | - | + | - | - | |
42.602 | 十五烷(Pentadecane) | + | + | - | - | |
43.311 | 环氧十二烷(13-Oxabicyclo[10.1.0]tridecane) | - | + | + | - | |
44.413 | 二十烷(Eicosane) | - | + | - | - | |
44.907 | 二十六烷(Hexacosane) | - | + | - | - | |
46.122 | 1,2-环氧十六烷(Oxirane, tetradecyl-) | - | + | - | - | |
47.129 | 十八烷(Octadecanal) | + | - | - | + | |
47.588 | 三癸基环氧乙烷(Oxirane, tridecyl-) | - | + | - | - | |
48.076 | 壬烷(9-Oxabicyclo[6.1.0]nonane) | - | + | - | + | |
49.506 | 二十八烷(Octacosane) | - | + | + | + | |
54.140 | 四十四烷(Tetratetracontane) | + | - | - | + | |
55.155 | 1,2-环氧环辛烷(9-Oxabicyclo[6.1.0]nonane, cis-) | - | - | + | - | |
62.616 | 氯代十六烷(Hexadecane, 1-chloro-) | - | + | + | - | |
67.922 | 十九烷(Nonadecane) | - | + | - | - | |
74.300 | 1,2-环氧十六烷(Oxirane, tetradecyl-) | + | - | - | - | |
74.382 | 胆甾烷 [Cholestane, 4,5-epoxy-, (4.alpha.,5.alpha.)-] | - | + | + | + | |
酯类 | 30.161 | 丙氨酸乙酯(D-Alanine, N-ethoxycarbonyl-, ethyl ester) | - | + | - | - |
36.745 | 亚硫酸,十八烷基2-丙酯(Sulfurous acid, octadecyl 2-propyl ester) | - | + | - | + | |
37.103 | 棕榈酸甲酯(Hexadecanoic acid, methyl ester) | + | - | - | + | |
37.689 | 邻苯二甲酸,壬酯(Phthalic acid, nonyl pentadecyl ester) | - | - | - | + | |
38.748 | 2-己基-1-癸醇硫酸氢酯(1-Decanol, 2-hexyl-) | - | + | - | - | |
45.614 | 乙酸正十七烷基酯(Heptadecyl acetate) | + | + | + | + | |
53.151 | 十七碳二烯酸甲酯(Methyl 9,12-heptadecadienoate) | - | - | + | - | |
53.643 | 9,12-十八酸(Z,Z)-, 2-羟基-1-(羟甲基)乙基酯 [9,12-Octadecadienoic acid (Z,Z)-, 2-hydroxy-1- (hydroxymethyl)ethyl ester] | - | - | + | - | |
酸类 | 38.009 | 2-溴十六烷酸(n-Hexadecanoic acid) | + | + | + | + |
48.979 | 油酸(Oleic Acid) | + | + | + | + | |
50.097 | 反式-13-十八碳烯酸(trans-13-Octadecenoic acid) | - | - | + | - | |
54.664 | 顺式-十八碳烯酸(cis-Vaccenic acid) | - | + | - | + | |
53.335 | 十七酸(Heptadecanoic acid, heptadecyl ester) | - | + | - | ||
69.995 | 11,13-二甲基-12-顺醋酸(11,13-Dimethyl-12-tetradecen-1-ol acetate) | + | - | - | + | |
烯烃类 | 35.566 | 新植二烯(Neophytadiene) | + | + | + | + |
51.434 | 6,11-二甲基-2,6,10-十二碳三烯(6,11-Dimethyl-2,6,10-dodecatrien-1-ol) | + | + | + | + | |
59.674 | 17-三十五烯(17-Pentatriacontene) | - | + | - | - | |
63.776 | 1,E-11,Z-13-十八碳三烯(1,E-11,Z-13-Octadecatriene) | - | + | - | + | |
其他类 | 43.974 | 油酸酰胺 [9-Octadecenamide, (Z)-] | - | - | - | + |
5.314 | 氯丙氧基苯 [benzene, 1-phenoxy-3- (4-phenoxyphenoxy)-] | - | + | - | - | |
类别 Types | 保留时间Retention time /min | 化学成分 Chemical components | 超声-乙醇 Ultrasound -ethanol | 超声-乙酸乙酯 Ultrasound-ethyl acetate | 超声-石油醚 Ultrasound- petroleum ether | 超临界CO2 Supercritical CO2 |
其他类 | 39.823 | 2,2-亚甲基双-(4-甲基-6-叔丁基苯酚) Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl- | + | + | - | - |
40.127 | 鲸蜡基戊烷基醚(Hexadecyl pentyl ether) | + | - | + | + | |
51.011 | 十八烷基乙烯基醚 [Octadecane, 1-(ethenyloxy)-] | - | - | + | - | |
40.814 | 聚酰胺树 [9,12-Octadecadienoic acid (Z,Z)-] | + | + | + | + | |
48.471 | 9,17-十八二烯醛 [9,17-Octadecadienal, (Z)-] | + | + | + | + | |
52.119 | 亚麻酰氯 [9,12-Octadecadienoyl chloride, (Z,Z)-] | - | + | + | + | |
54.175 | 12-甲基-反,反-2,13-十八碳二烯-1-醇 (12-Methyl-E,E-2,13-octadecadien-1-ol) | - | + | + | - | |
59.972 | 苯并二氧六环(1,4 Benzodioxan-6-amine) | + | - | + | + | |
61.529 | 正十八烷-5,4-二丁基(Octadecane, 5,14-dibutyl-) | - | + | - | - | |
70.032 | 顺,顺-2-甲基-3,13-十八二烯醇(2-Methyl-Z,Z-3,13-octadecadienol) | - | + | + | - |
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