Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 579-585.DOI: 10.3969/j.issn.1000-2561.2020.03.022

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Antioxidant Activities and GC-MS Analysis of Ilex kudingcha C.J. Tseng Extracts by Different Extraction Methods

ZHU Kexue1,2,DAO Chunli1,3,GU Wenliang1,XU Fei1,TAN Lehe1,2,*()   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
    2. Hainan Provincial Engineering Research Center for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
    3. College of Tropical Crop Science, Yunnan Agricultural University, Pu’er, Yunnan 665000, China;
  • Received:2019-05-29 Revised:2019-08-14 Online:2020-03-25 Published:2020-04-16
  • Contact: TAN Lehe


The present study was aimed to compare the effects of the extracts of Ilex kudingcha C.J. Tseng with different organic solvents (ethanol, ethyl acetate, petroleum ether) assisted by ultrasound and supercritical CO2 extraction against oxidation activities (DPPH?) scavenging activity, hydroxyl radical (?OH) elimination activity, superoxide anion free radical (O2 -?) scavenging activity and ABTS +? radical elimination ability), and component analysis by gas chromatography-mass spectrometry (GC-MS). The DPPH? and O2 -? scavenging abilities were as follows: ultrasound-ethanol extract > supercritical CO2 extract > ultrasound-ethyl acetate > ultrasound-petroleum ether extract. The OH? and ABTS +? radical scavenging abilities were as follows: ultrasound-ethanol extract > ultrasound-ethyl acetate extract > supercritical CO2 extract > ultrasound-petroleum ether extract. GC-MS analysis showed that there were five main types of substances in the four different extraction methods, a total of 50 chemical components and seven common components.

Key words: Ilex kudingcha C.J. Tseng, extracts, supercritical CO2, in vitro antioxidant activity, GC-MS

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