Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 315-322.DOI: 10.3969/j.issn.1000-2561.2020.02.015

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning and Expression of Cyclophilin Gene PhCyP from Phalaenopsis

YUAN Xiuyun,XU Shenping,ZHANG Yan,WANG Mofei,JIANG Suhua,LIANG Fang,CUI Bo()   

  1. Institute of Bioengineering, Zhengzhou Normal University, Zhengzhou, Henan 450044, China
  • Received:2019-04-30 Revised:2019-06-27 Online:2020-02-25 Published:2020-03-21
  • Contact: CUI Bo


To study the molecular regulation mechanism of Phalaenopsis in response to low temperature stress, a cyclophilin gene PhCyP (GenBank accession number: MH992514) was cloned from the leaf of Phalaenopsis through the RT-PCR and RACE method. The full-length cDNA sequence was 829 bp with an open reading frame (ORF) of 522 bp encoding 173 amino acids. PhCyP contained a cyclophilin-like domain (CLD) with alkaline and hydrophilic properties. The phylogenetic tree showed that PhCyP was closely related to the cyclophilin from Phalaenopsis equestris, Vanda hybrid cultivar, Apostasia shenzhenica and Dendrobium catenatum in Orchidaceae. The expression analysis showed that PhCyP was highly expressed in different tissues including root, leaf, flower, ovary and seed in different development stage. Under 13 ℃/8 ℃ day/night temperature, the expression level of PhCyP reduced gradually with treatment time and was the lowest at 6 d, then increased gradually from 9 d to restoring. Under 4 ℃ treatment, the expression level of PhCyP increased gradually, and achieved the highest level at 4 h, then its expression level decreased gradually with a lower level than that before treatment at 48 h. The results suggested that PhCyP was involved in the response to low temperature stress with different molecular regulation mechanism to different low temperature stress.

Key words: Phalaenopsis spp., low temperature, cyclophilin, gene expression

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