Effects of Exogenous Ca 2+ on Leaf Physiological Indexes in Black Pepper Under Low Temperature Stress

doi:10.3969/j.issn.1000-2561.2020.02.009

Abstract

Abstract:

Piper nigrum cv. Reyin1 is widely planted in China and is sensitive to cold stress. Five different concentrations of calcium chloride treatments including 0, 7, 14, 21, 28 mmol/L were set to spray black pepper. All the materials were treated with low temperature (10 ℃/5 ℃, 12 h/12 h) for four days and recovery training (28 ℃/20 ℃, 12 h/12 h) for six days. The phenotype and physiological indexes were analyzed. Calcium chloride treatment of 7 mmol/L could improve the cold damage phenotype and net photosynthetic rate of black pepper. With Ca ²⁺ concentration increasing, the cold damage phenotype gradually aggravated and net photosynthetic rate became lower. Compared with CK, the 7 mmol/L Ca ²⁺ treatment could significantly improve the activity of antioxidant enzymes, increase the soluble sugar content and reduce the malondialdehyde content. With the Ca ²⁺ treatment concentration increasing, the activity of antioxidant enzymes and the soluble sugar content became lower, and the malondialdehyde content became higher. The results would provide a reference for cold resistance technical guidance and cold resistance molecular breeding in black pepper production.

Key words: Piper nigrum cv. Reyin1, calcium chloride, cold stress

CLC Number:

Q949.732.3

WU Baoduo,TANG Hu,HU Lisong,FAN Rui,YANG Jianfeng,ZHOU Yanfei,HAO Chaoyun. Effects of Exogenous Ca ²⁺ on Leaf Physiological Indexes in Black Pepper Under Low Temperature Stress[J]. Chinese Journal of Tropical Crops, 2020, 41(2): 267-274.

Figures/Tables 6

Tab. 1 Effects of calcium chloride treatment on photosynthetic parameters of pepper

处理 Treatment	P_n/(μmol·m^-2·s^-1)		G_s/(mol·m^-2·s^-1)		T_r/(mmol·m^-2·s^-1)		C_i/(μmol·mol^-1)
处理 Treatment	低温胁迫前 Before low temperature stress	低温胁迫后 After low temperature stress	低温胁迫前 Before low temperature stress	低温胁迫后 After low temperature stress	低温胁迫前 Before low temperature stress	低温胁迫后 After low temperature stress	低温胁迫前 Before low temperature stress	低温胁迫后 After low temperature stress
CK	4.44±0.17^a	0.78±0.20^b	0.06±0.02^a	0.02±0.01^a	244.51±41.71^a	325.48±29.33^b	1.26±0.38^a	0.37±0.11^a
T1	4.68±0.36^a	1.39±0.24^a	0.06±0.02^a	0.03±0.01^a	260.92±29.01^a	307.37±12.82^c	1.33±0.31^a	0.46±0.15^a
T2	4.42±0.45^a	0.76±0.19^b	0.05±0.01^a	0.02±0.00^a	239.03±14.72^a	324.75±12.05^bc	1.13±0.21^a	0.29±0.02^a
T3	3.84±0.29^b	0.83±0.21^b	0.05±0.01^a	0.03±0.00^a	253.10±20.19^a	342.96±12.61^b	1.11±0.27^a	0.40±0.04^a
T4	1.20±0.10^c	0.05±0.02^c	0.01±0.00^b	0.02±0.00^a	248.90±9.91^a	385.39±1.19^a	0.36±0.00^b	0.34±0.06^a

Fig. 1 Plant phenotype of P. nigrum cv. Reyin1 treated by different concentration of CaCl2 under cold treatment

Fig. 2 Ca2+-ATPase activities of P. nigrum cv. Reyin1 treated by different concentration of CaCl2 under low temperature stress Different lowercase letters indicate significant difference at 0.05 level.

Fig. 3 SOD, CAT, POD activities of P. nigrum cv. Reyin1 treated by different concentration of CaCl2 under low temperature stress Different lowercase letters indicate significant difference at 0.05 level.

Fig. 4 Soluble protein and sugar contents of P. nigrum cv. Reyin1 treated by different concentration of CaCl2 under low temperature stress Different lowercase letters indicate significant difference at 0.05 level.

Fig.5 MDA and Pro contents of P. nigrum cv. Reyin1 treated by different concentration of CaCl2 under low temperature stress Different lowercase letters indicate significant difference at 0.05 level.

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Effects of Exogenous Ca ²⁺ on Leaf Physiological Indexes in Black Pepper Under Low Temperature Stress

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