Comparison of Dissolved Organic Matter from Biochar on Attenuating Biotoxicity of Oxytetracycline

doi:10.3969/j.issn.1000-2561.2019.12.024

Abstract

Abstract:

In order to explore and state the possibility effects of dissolved organic matter (DOM) on the biotoxicity of oxytetracycline (OTC), aqueous solution and soil culture incubation experiments were conducted to investigate the effects of DOM extracted from biochar, derived from the cassava residue at 350 ℃, 500 ℃, 650 ℃, on the pakchoi (Brassica rapa var. glabra) seed germination, root and bud elongation. Results showed that DOM350 and DOM500 had a promoting effect on seed germination rate of Brassica rapa var. glabra in hydroponic and soil culture condition, which was 3.33% and 10.00%, respectively. For DOM-OTC system, there was a significant correlation between DOM concentrations and bud elongation of pakchoi (B. rapa var. glabra) seeds (P<0.05). Furthermore, DOM had a synergistic effect with low concentration (<34.78 mg/kg) of OTC to promote root and bud extension of seeds, and antagonized the biotoxicity of OTC with high concentration (>94.09 mg/kg) of OTC. However, DOM alleviated the toxicity of OTC on root elongation rate, and had a highly significant effect on shoot elongation of seeds cultured in water (P<0.01), when DOC concentration at 10 mg/L.

Key words: dissolved organic matter, oxytetracycline, Brassica rapa var. glabra, biotoxicity, biochar

CLC Number:

GE Chengjun,ZHAO Yuanyuan,YU Huamei,YUE Lin,FU Yue,MA Ruiyang,CHENG Lei. Comparison of Dissolved Organic Matter from Biochar on Attenuating Biotoxicity of Oxytetracycline[J]. Chinese Journal of Tropical Crops, 2019, 40(12): 2489-2497.

Figures/Tables 12

Fig. 1 Germination rate of pakchoi under OTC stress Different lowercase letters mean significant difference (P<0.05).

Fig. 2 Germination rate of pakchoi with DOM addition

Fig. 3 Germination rate of co-existence of DOM and OTC under water culturing Different lowercase letters mean significant difference (P <0.05).

Fig. 4 Germination rate of co-existence of DOM and OTC under soil culturing

Fig. 5 Effect of co-existence OTC and DOM on the inhibition rate of root elongation under hydroponic condition

Tab. 1 Regression equation for seed root elongation under the effect of DOM and oxytetracycline stress grown in water

样品 Samples	拟合方程 Regression equation	R²	P
DOM350	RI=0.0091X²-0.5427X+72.893	0.8045	P>0.05
DOM500	RI=0.0129X²-0.6624X+72.433	0.7491	P>0.05
DOM650	RI=0.0081X²-0.3399X+73.721	0.9898	P<0.01

Fig. 6 Effect of co-existence OTC and DOM on the inhibition rate of root elongation under soil culturing

Tab. 2 Regression equation and IC50 for seed root elongation under the effect of DOM and oxytetracycline stress

样品 Samples	拟合方程 Regression equation	R²	IC₅₀	P
CK	RI=0.609X-13.330	0.8920	103.99	<0.01
DOM350	RI=0.416X-18.541	0.8080	164.76	<0.05
DOM500	RI=0.259X-24.369	0.8756	287.14	<0.01
DOM650	RI=0.508X-17.668	0.8560	133.20	<0.01

Fig. 7 Effect of co-existence OTC and DOM on the inhibition rate of shoot elongation under hydroponic condition

Tab. 3 Regression equation for shoot elongation under the stress of DOM and oxytetracycline under hydroponic condition

样品 Samples	拟合方程 Fitting equation	R²	P	n
DOM350	RI=-1.1166X+17.975	0.8224	P<0.01	12
DOM500	RI=-1.2036X+22.753	0.9302	P<0.01	12
DOM650	RI=-1.5691X+12.567	0.8230	P<0.01	12

Fig. 8 Effect of co-existence OTC and DOM on the inhibition rate of shoot elongation under soil culturing

Tab. 4 Regression equation and IC50 for shoot elongation under the stress of DOM and oxytetracycline under soil culturing

样品 Samples	拟合方程 Fitting equation	R²	IC₅₀	P
CK	RI=0.0862X-7.9438	0.8825	672.20	P<0.01
DOM350	RI=0.0808X-13.379	0.9340	784.39	P<0.01
DOM500	RI=0.0461X-16.468	0.8466	1441.82	P<0.01
DOM650	RI=0.0749X-15.232	0.8449	870.92	P<0.01

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