Analysis on Volatiles of Oolong Tea Varieties Induced by Empoasca sp.

doi:10.3969/j.issn.1000-2561.2019.03.023

Abstract

Abstract:

The changes of tea plant volatiles induced by tea green leafhopper Empoasca sp. were determined to provide basis for breeding of resistant tea varieties. Based on the field observation of Empoasca sp. on different tea varieties from Fujian province, Rougui and Tieguanyin were found to have the highest population density and the lowest population density of Empoasca sp. respectively, and selected as the research materials in this paper. Gas chromatography-mass spectrometer (GC-MS) was used to determine and analyze constitutive volatiles of tea and induced volatiles by Empoasca sp. The results showed that P-Cymene, beta.-Ocimene, Trans.-beta.-Ocimene, alpha.-Farnesene, gamma.-Terpineneand and 3-Methyl-3-cyclohexen-1-one were the main constituent volatiles of Tieguanyin and Rougui. The 1,6-Octadien-3-ol, 3,7-dimethyl- was detected only in the health shoots of Tieguanyin, while Tridecane, Acetic acid octyl ester, Hexadecane and Cedrol were be detected only in Rougui. The content of beta.-Myrcene, beta.-Ocimene, Trans.-beta.-Ocimene, alpha.-Farnesene and 1,6-Octadien-3-ol, 3,7-dimethyl were far higher than that of Rougui. In combination with other literature, it is inferred that these volatile chemicals possibly resulted in the significant difference in the population density of Empoasca sp. between Tieguanyin and Rougui. After a period of damage by Empoasca sp., the composition and content of volatiles in Tieguanyin and Rougui changed greatly. Both of them released large amounts of beta.-Myrcene, beta.-Ocimene, Trans.-beta.-Ocimene and alpha.-Farnesene, the contents of these chemicals largely increased in 4 h and 8 h damage treatments with Empoasca sp. ranged from 1.49 to 41.22 times than them released from health tea shoots. In addition, Empoasca sp. feeding also induced new volatile chemical including (Z)-Butanoic acid 3-hexenyl ester, Decanal, Indole, Hexanoicacid 3-hexenyl ester, and phenylethyl alcohol. The 10 volatile chemicals mentioned above may be related to the induced resistance of tea plant and the resistance of Tieguanyin against to Empoasca sp.

Key words: Camellia sinensis (L.) O. Ktze., Empoasca sp., volatile components, resistance

JIN Shan,HAN Liwei,YE Naixing,WANG Wei,HUANG Huoshui,LIU Wei. Analysis on Volatiles of Oolong Tea Varieties Induced by Empoasca sp.[J]. Chinese Journal of Tropical Crops, 2019, 40(3): 576-582.

Figures/Tables 4

References 22

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序号 No.	化合物名称 Name of compound	铁观音（相对于内标的含量） Tieguanyin (Relative contents to internal standard, %)				肉桂（相对于内标的含量） Rougui (Relative contents to internal standard, %)
		健康对照Contrast		虫害处理Treatment		健康对照Contrast		虫害处理Treatment
		4 h	8 h	4 h	8 h	4 h	8 h	4 h	8 h
1	β-月桂烯 beta.-Myrcene	0.86±0.04^b	1.82±0.11^a	3.78±0.31^a	3.38±0.25^a	0.28±0.09^a	0.33±0.07^a	7.07±1.07^a	6.10±1.85^a
2	柠檬烯 D-Limonene	0.34±0.04^b	0.81±0.09^a	—	—	0.30	—	—	—
3	邻异丙基苯甲烷 p-Cymene	1.37±0.19^b	3.30±0.49^a	1.18±0.14^b	3.68±0.55^a	1.27±0.48^a	2.32±0.77^a	0.95±0.19^b	2.66±0.54^a
4	苯甲醛 Benzaldehyde	0.23±0.06^a	0.13±0.05^a	0.74±0.09^a	0.81±0.05^a	0.12±0.05^a	0.21±0.07^a	0.07±0.02^b	0.33±0.06^a
5	反式-β-罗勒烯 Trans.- beta.- Ocimene	1.92±0.23^b	3.89±0.37^a	8.78±1.23^a	8.78±1.31^a	0.40±0.07^a	0.00±0.00^b	16.49±3.24^a	11.03±1.44^b
6	β-罗勒烯 beta.-Ocimene	153.54±14.19^b	259.67±21.68^a	285.57±13.14^b	387.66±26.91^a	39.62±5.56^a	26.23±4.03^a	471.98±34.24^a	309.39±27.50^b
7	γ-萜品烯 gamma.- Terpinene	1.30	—	0.16	—	0.31±0.06^a	0.43±0.06^a	0.30	—
8	芳樟醇 1,6-Octadien-3-ol, 3,7- dimethyl-	0.23	—	2.43±0.53^a	1.71±0.44^a	—	—	12.39±2.96^a	1.79±0.52^b
9	苯乙醇 Phenylethyl Alcohol	—	—	0.12±0.02^a	0.11±0.02^a	—	—	0.26±0.05^a	0.27±0.05^a
10	十二烷 Dodecane	0.16±0.05^a	0.17±0.03^a	0.21±0.04^a	0.25±0.05^a	0.15±0.02^a	0.14±0.02^a	0.14	—
11	3-甲基-3-环己烯-1-酮 3-Methyl-3-cyclohexen-1-one	21.30±2.96^a	26.46±5.01^a	—	—	27.26±3.73^a	18.37±3.12^a	—	—
12	(Z)-丁酸-3- 己烯酯 Butanoic acid, 3-hexenyl ester, (Z)-	—	—	0.45±0.08^a	0.17±0.07^a	—	—	2.50±0.69^a	0.37±0.32^a
13	癸醛Decanal	—	—	0.16±0.04^a	0.32±0.05^a	—	—	0.08	—
14	吲哚Indole	—	—	0.75±0.10^a	0.35±0.09^b	—	—	1.90±0.25^a	0.62±0.19^b
15	十三（碳）烷 Tridecane	—	—	0.25±0.04^a	0.18±0.04^a	0.06±0.03^a	0.05±0.00^a	—	—
16	乙酸辛酯 Acetic acid, octyl ester	—	—	0.09±0.02^a	0.12±0.05^a	0.06±0.02^a	0.04±0.02^a	0.09	—
17	石竹烯Caryophyllene	0.64±0.05^b	1.54±0.24^a	—	—	0.21±0.04^b	0.47±0.05^a	—	—
18	α-法呢烯 alpha.- Farnesene	8.45±1.74^b	17.96±2.06^a	27.87±3.98^b	59.90±8.40^a	—	1.25	43.08±6.91^a	45.64±6.76^a
19	己酸-3-己烯酯 Hexanoic acid, 3-hexenyl ester	—	—	0.41±0.06^a	0.12±0.04^b	—	—	5.28±0.60^a	1.01±0.17^b
20	十六烷 Hexadecane	—	—	0.19±0.07^a	0.30±0.09^a	0.07±0.02^a	0.06±0.02^a	0.15	—
21	雪松醇Cedrol	—	—	0.20±0.06^a	0.28±0.07^a	—	0.08	—	0.17

序号 No.	化合物名称 Name of compound	铁观音（相对于内标的含量） Tieguanyin (Relative contents to internal standard, %)				肉桂（相对于内标的含量） Rougui (Relative contents to internal standard, %)
		健康对照Contrast		虫害处理Treatment		健康对照Contrast		虫害处理Treatment
		4 h	8 h	4 h	8 h	4 h	8 h	4 h	8 h
1	β-月桂烯 beta.-Myrcene	0.86±0.04^b	1.82±0.11^a	3.78±0.31^a	3.38±0.25^a	0.28±0.09^a	0.33±0.07^a	7.07±1.07^a	6.10±1.85^a
2	柠檬烯 D-Limonene	0.34±0.04^b	0.81±0.09^a	—	—	0.30	—	—	—
3	邻异丙基苯甲烷 p-Cymene	1.37±0.19^b	3.30±0.49^a	1.18±0.14^b	3.68±0.55^a	1.27±0.48^a	2.32±0.77^a	0.95±0.19^b	2.66±0.54^a
4	苯甲醛 Benzaldehyde	0.23±0.06^a	0.13±0.05^a	0.74±0.09^a	0.81±0.05^a	0.12±0.05^a	0.21±0.07^a	0.07±0.02^b	0.33±0.06^a
5	反式-β-罗勒烯 Trans.- beta.- Ocimene	1.92±0.23^b	3.89±0.37^a	8.78±1.23^a	8.78±1.31^a	0.40±0.07^a	0.00±0.00^b	16.49±3.24^a	11.03±1.44^b
6	β-罗勒烯 beta.-Ocimene	153.54±14.19^b	259.67±21.68^a	285.57±13.14^b	387.66±26.91^a	39.62±5.56^a	26.23±4.03^a	471.98±34.24^a	309.39±27.50^b
7	γ-萜品烯 gamma.- Terpinene	1.30	—	0.16	—	0.31±0.06^a	0.43±0.06^a	0.30	—
8	芳樟醇 1,6-Octadien-3-ol, 3,7- dimethyl-	0.23	—	2.43±0.53^a	1.71±0.44^a	—	—	12.39±2.96^a	1.79±0.52^b
9	苯乙醇 Phenylethyl Alcohol	—	—	0.12±0.02^a	0.11±0.02^a	—	—	0.26±0.05^a	0.27±0.05^a
10	十二烷 Dodecane	0.16±0.05^a	0.17±0.03^a	0.21±0.04^a	0.25±0.05^a	0.15±0.02^a	0.14±0.02^a	0.14	—
11	3-甲基-3-环己烯-1-酮 3-Methyl-3-cyclohexen-1-one	21.30±2.96^a	26.46±5.01^a	—	—	27.26±3.73^a	18.37±3.12^a	—	—
12	(Z)-丁酸-3- 己烯酯 Butanoic acid, 3-hexenyl ester, (Z)-	—	—	0.45±0.08^a	0.17±0.07^a	—	—	2.50±0.69^a	0.37±0.32^a
13	癸醛Decanal	—	—	0.16±0.04^a	0.32±0.05^a	—	—	0.08	—
14	吲哚Indole	—	—	0.75±0.10^a	0.35±0.09^b	—	—	1.90±0.25^a	0.62±0.19^b
15	十三（碳）烷 Tridecane	—	—	0.25±0.04^a	0.18±0.04^a	0.06±0.03^a	0.05±0.00^a	—	—
16	乙酸辛酯 Acetic acid, octyl ester	—	—	0.09±0.02^a	0.12±0.05^a	0.06±0.02^a	0.04±0.02^a	0.09	—
17	石竹烯Caryophyllene	0.64±0.05^b	1.54±0.24^a	—	—	0.21±0.04^b	0.47±0.05^a	—	—
18	α-法呢烯 alpha.- Farnesene	8.45±1.74^b	17.96±2.06^a	27.87±3.98^b	59.90±8.40^a	—	1.25	43.08±6.91^a	45.64±6.76^a
19	己酸-3-己烯酯 Hexanoic acid, 3-hexenyl ester	—	—	0.41±0.06^a	0.12±0.04^b	—	—	5.28±0.60^a	1.01±0.17^b
20	十六烷 Hexadecane	—	—	0.19±0.07^a	0.30±0.09^a	0.07±0.02^a	0.06±0.02^a	0.15	—
21	雪松醇Cedrol	—	—	0.20±0.06^a	0.28±0.07^a	—	0.08	—	0.17