Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 708-714.DOI: 10.3969/j.issn.1000-2561.2019.04.013

• Biotechnology and Tissue Cultures • Previous Articles     Next Articles

Construction of DNA Fingerprinting with SSR Markers for 68 Rambutan (Nephelium lappaceum L.) Accessions

LIN Xing’e1,NIU Junhai2,CHEN Ying3,MING Jianhong1,GAO Hongmao1,GE Yu1,ZHOU Zhaoxi1,*()   

  1. 1. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 570102, China
    2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China
    3. Institute of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
  • Received:2018-06-27 Revised:2019-02-13 Online:2019-04-15 Published:2019-05-21
  • Contact: Zhaoxi ZHOU


The SSR fingerprint map and genetic diversity of 68 rambutan (Nephelium lappaceum L.) accessions were studied using 10 pairs of selected polymorphic SSR primers. The results indicated that all the primers showed polymorphism, and 20 polymorphic alleles were revealed and the average polymorphic information content (PIC) was 0.393. The genetic similarities among accessions ranged from 0.290 to 0.664, on which a phylogenetic tree showing the relationship among the accessions was constructed. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 68 cultivars. The DNA fingerprinting provides a technical reference for the varieties identification and molecular breeding in rambutan.

Key words: Nephelium lappaceum L., SSR, DNA fingerprint