Antifungal Activity of 13 Phenylpropanoid Metabolites Against Six Colletotrichum Species

doi:10.3969/j.issn.1000-2561.2022.12.014

Abstract

Abstract:

Colletotrichum is a fungal pathogen which causes a destructive disease called anthracnose and affects a wide range of tropical crop plants. Development of green pesticide is important for the prevention and control of anthracnose disease. Early transcriptome and metabolome studies of stylo (Stylosanthes spp.) have shown that the phenylpropanoid metabolic pathway is significantly up-regulated in response to Colletotrichum infection. In this study, an in vitro antifungal test was used to determine the inhibitory effects of 13 phenylpropanoid metabolites on the mycelial growth of six Colletotrichum species, including stylo C. gloeosporioides, rubber C. gloeosporioides, rubber C. acutatum, yam C. gloeosporioides, mango C. gloeosporioides and papaya C. gloeosporioides. The metabolites with higher inhibitory effects and the pairwise combinations were used to test the activity on the inhibition of the mycelial growth of six Colletotrichum species. In addition, the effects on conidial germination of five Colletotrichum species were also examined using the metabolites and their pairwise combinations. The results showed that 13 metabolites displayed different inhibitory effects on the six Colletotrichum species. Among all the tested metabolites, pterostilbene had the best inhibitory effect on the mycelial growth of the six Colletotrichum species at both 500 μmol/L and 1000 μmol/L. The inhibition rates of pterostilbene ranged from 47.47% to 80.74% at 1000 μmol/L. Phloretin and coumarin also showed inhibitory effects on the mycelial growth of the six Colletotrichum species at a 1000 μmol/L, but the rest nine metabolites did not show significant inhibitory effects. Then, pterostilbene, phloretin, coumarin, ferulic acid, naringin, resveratrol were combined in pair to test the effects on the mycelial growth and conidial germination of the six Colletotrichum. The results showed the combinations containing pterostilbene had a significant inhibitory effect on the mycelial growth of the six Colletotrichum species, and the inhibition rates ranging from 31.07% to 89.05%. For the conidial germination, pterostilbene and its combinations showed a significant inhibitory effect on five Colletotrichum. Therefore, the IC₅₀ values of pterostilbene and its combinations on conidial germination of five Colletotrichum species were determined. Overall, the metabolites or combinations with best inhibition effects on stylo C. gloeosporioides, rubber C. gloeosporioides, rubber C. acutatum, mango C. gloeosporioides and papaya C. gloeosporioides were pterostilbene+phloretin, pterostilbene+ferulic acid, pterostilbene+ferulic acid, pterostilbene+phloretin and pterostilbene with IC₅₀ values of 293.475 μmol/L, 67.660 μmol/L, 184.764 μmol/L, 108.671 μmol/L and 68.417 μmol/L, respectively. The metabolites and its combination identified from this study could provide candidates for further development of green pesticide.

Key words: phenylpropanoid metabolite, Colletotrichum, antifungal activity

CLC Number:

S432.1

WU Pengpeng, AN Wei, XU Yunfeng, LUO Lijuan, JIANG Lingyan. Antifungal Activity of 13 Phenylpropanoid Metabolites Against Six Colletotrichum Species[J]. Chinese Journal of Tropical Crops, 2022, 43(12): 2515-2526.

Figures/Tables 5

References 38

[1]	CANNON P F, DAMM U, JOHNSTON P R, WEIR B S. Colletotrichum-current status and future directions[J]. Studies in Mycology, 2012, 73: 181-213.
[2]	李少卡, 赵亚, 王祥和, 胡福初, 陈哲, 范鸿雁. 海南荔枝炭疽病病原菌鉴定及遗传多样性分析[J]. 农业生物技术学报, 2021, 29(4): 673-687.
	LI S K, ZHAO Y, WANG X H, HU F C, CHEN Z, FAN H Y. Identification and genetic diversity analysis of Litchi chinensis Colletotrichum spp. in Hainan[J]. Journal of Agricultural Biotechnology, 2021, 29(4): 673-687. (in Chinese)
[3]	李其利, 卜俊燕, 唐利华, 黄穗萍, 郭堂勋, 莫贱友. 芒果炭疽菌研究进展[J]. 微生物学杂志, 2020, 40(1): 117-124.
	LI Q L, BU J Y, TANG L H, HUANG S P, GUO T X, MO J Y. Advances in mango anthracnose (Colletotrichum gloeosporioides)[J]. Journal of Microbiology, 2020, 40(1): 117-124. (in Chinese)
[4]	蔡志英, 李加智, 王进强, 张春霞, 何明霞, 贺丽琼. 橡胶胶孢炭疽菌和尖孢炭疽菌对杀菌剂的敏感性测定[J]. 云南农业大学学报, 2008, 23(6): 787-790.
	CAI Z Y, LI J Z, WANG J Q, ZHANG C X, HE M X, HE L Q. Sensitivity test of Colletotrichum gloeosporioides and Colletotrichum acutatum isolated from rubber to the fungicides[J]. Journal of Yunnan Agricultural University, 2008, 23(6): 787-790. (in Chinese)
[5]	易克贤. 柱花草炭疽病及其抗病育种进展[J]. 中国草地, 2001, 23(4): 59-65.
	YI K X. Stylo anthracnose and current research progresses on anthracnose resistance breeding[J]. Grassland China, 2001, 23(4): 59-65. (in Chinese)
[6]	DEAN R, KAN J, PRETORIUS Z A, HAMMOND- KOSACK K E, PIETRO A D, SPANU P D, RUDD J J, DICKMAN M, KAHMANN R, ELLIS J. The Top 10 fungal pathogens in molecular plant pathology[J]. Molecular Plant Pathology, 2012, 13(7): 804.
[7]	刘丽萍, 高洁, 李玉. 植物炭疽菌属Colletotrichum真菌研究进展[J]. 菌物研究, 2020, 18(4): 266-281.
	LIU L P, GAO J, LI Y. Advances in knowledge of the fungi referred to the genus Colletotrichum[J]. Journal of Fungal Research, 2020, 18(4): 266-281. (in Chinese)
[8]	李河, 李司政, 王悦辰, 刘君昂, 徐建平, 周国英. 油茶苗圃炭疽病原菌鉴定及抗药性[J]. 林业科学, 2019, 55(5): 85-94.
	LI H, LI S Z, WANG Y C, LIU J A, XU J P, ZHOU G Y. Identification of the pathogens causing anthracnose of Camellia oleifera in nursery and their resistence to fungicides[J]. Scienta Silve Sinicae, 2019, 55(5): 85-94. (in Chinese)
[9]	张正炜, 郗厚诚, 常文程, 黄璐璐, 陈秀. 我国植物源农药商品化应用现状及产业发展建议[J]. 世界农药, 2020, 42(12): 6-15.
	ZHANG Z W, XI H C, CHANG W C, HUANG L L, CHEN X. Current situation of commercialized application of plant-derived pesticides in China and suggestions for industrial development[J]. World Pesticides, 2020, 42(12): 6-15. (in Chinese)
[10]	王忠兴, 魏芸娜, 顾沛雯. 几种植物源和微生物源药剂对葡萄灰霉菌的毒力测定及田间防效[J]. 北方园艺, 2019(15): 55-60.
	WANG Z X, WEI Y N, GU P W. The toxicity and control effect of several botanical and biological's pesticides against Botrytis cinerea[J]. Northern Horticulture, 2019(15): 55-60. (in Chinese)
[11]	石洁, 李宝燕, 栾炳辉, 李凌云, 田园园, 王英姿. 5种生物药剂对葡萄炭疽病药效研究[J]. 中国果树, 2021(1): 74-76.
	SHI J, LI B Y, LUAN B H, LI L Y, TIAN Y Y, WANG Y Z. Study on the control effect of five biological fungicides on grape anthracnose[J]. China Fruits, 2021(1): 74-76. (in Chinese)
[12]	DONG N Q, LIN H X. Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions[J]. Journal of Integrative Plant Biology, 2021, 63(1): 180-209.
[13]	NAOUMKINA M A, ZHAO Q, GALLEGO‐GIRALDO L, DAI X, ZHAO P X, DIXON R A. Genome-wide analysis of phenylpropanoid defence pathways[J]. Molecular Plant Pathology, 2010, 11(6): 829-846. DOI PMID
[14]	BEYER S F, BEESLEY A, ROHMANN P F W, SCHULTHEISS H, LANGENBACH C. The Arabidopsis non-host defence-associated coumarin scopoletin protects soybean from Asian soybean rust[J]. The Plant Journal, 2019, 99(3): 397-413.
[15]	LIU X, CUI X, JI D, ZHANG Z, TIAN S. Luteolin-induced activation of the phenylpropanoid metabolic pathway contributes to quality maintenance and disease resistance of sweet cherry[J]. Food Chemistry, 2021, 342: 128309.
[16]	ZHANG M Y, WANG D J, GAO X X, YUE Z Y, ZHOU H L. Exogenous caffeic acid and epicatechin enhance resistance against Botrytis cinerea through activation of the phenylpropanoid pathway in apples[J]. Scientia Horticulturae, 2020, 268: 109348.
[17]	YANG L, WANG Y, HE X B, XIAO QL, HAN ST, JIA Z, LI S L, DING W. Discovery of a novel plant-derived agent against Ralstonia solanacearum by targeting the bacterial division protein FtsZ[J]. Pesticide Biochemistry and Physiology, 2021, 177: 104892.
[18]	YANG L, WEI Z L, LI S L, XIAO R, XU Q Q, RAN Y, DING W. Plant secondary metabolite, daphnetin reduces extracellular polysaccharides production and virulence factors of Ralstonia solanacearum[J]. Pesticide Biochemistry and Physiology, 2021, 179: 104948.
[19]	ROY S, NUCKLES E, ARCHBOLD D D. Effects of phenolic compounds on growth of Colletotrichum spp[J]. Current Microbiology, 2018, 75(5): 550-556.
[20]	JIANG L Y, WU P P, YANG L Y, LIU C, GUO P F, WANG H, WANG S C, XU F P, ZHUANG Q W, TONG X Z, LIU P D, LUO L J. Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides[J]. Genomics, 2021, 113(4): 2702-2716.
[21]	陈年春. 农药生物测定技术农药植保专业用[M]. 北京: 北京农业大学出版社, 1991.
	CHEN N C. Pesticide bioassay technology for pesticide plant protection specialty[M]. Beijing: Beijing Agricultural University Press, 1991. (in Chinese)
[22]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
	FANG Z D. Research methods of plant diseases[M]. 3rd edition. Beijing: China Agriculture Press, 1998. (in Chinese)
[23]	TALBOT N J, EBBOLE D J, HAMER J E. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea[J]. Plant Cell, 1993, 5(11): 1575-1590.
[24]	周子骞, 李敏, 高兆银, 王义, 洪小雨, 李春霞, 胡美姣. 海南番木瓜炭疽病病原菌鉴定及其防治药剂筛选[J]. 西南农业学报, 2020, 33(1): 70-76.
	ZHOU Z Q, LI M, GAO Z Y, WANG Y, HONG X Y, LI C X, HU M J. Pathogens identification and fungicide screening of papaya fruit anthrocnose in Hainan[J]. Southwest China Journal of Agricultural Sciences, 2020, 33(1): 70-76. (in Chinese)
[25]	曹学仁, 车海彦, 杨毅, 罗大全. 2014年海南省橡胶炭疽病菌对多菌灵和咪鲜胺的敏感性测定[J]. 植物病理学报, 2015, 45(6): 626-631.
	CAO X R, CHE H Y, YANG Y, LUO D Q. Sensitivity of Colletotrichum spp. from Hevea brasiliensis to carbendazim and prochloraz in Hainan province in China in 2014[J]. Acta Phytopathologica Sinica, 2015, 45(6): 626-631. (in Chinese)
[26]	王万东, 刘光华, 尼章光, 罗心平, 俞艳春. 芒果炭疽病的发生规律及综合防治[J]. 广东农业科学, 2008, 35(6): 67-69.
	WANG W D, LIU G H, NI Z G, LUO X P, YU Y C. Pathogenesis and comprehensive treatments of mango anthracnose[J]. Guangdong Agricultural Sciences, 2008, 35(6): 67-69. (in Chinese)
[27]	王雅君, 邵远志, 何文琪, 尤文静, 葛春晖, 李雯. 采后番木瓜果实炭疽病病原菌分离鉴定及其拮抗菌的筛选[J]. 食品工业科技, 2020, 41(6): 111-118, 130.
	WANG Y J, SHAO Y Z, HE W Q, YOU W J, GE C H, LI W. Isolation and identification of anthracnose pathogen in postharvest papaya fruits and screening of the antagonistic bacteria[J]. Science and Technology of Food Industry, 2020, 41(6): 111-118, 130. (in Chinese)
[28]	WINCH J E, NEWHOOK F J, JACKSON G V H, COLE J S. Studies of Colletotrichum gloeosporioides disease on yam, Dioscorea alata, in Solomon Islands[J]. Plant Pathology, 1984, 33(4): 467-477.
[29]	张俊有, 蒋家珍. 采用半果接种法研究几种杀菌剂对芒果炭疽病的防治效果[J]. 江苏农业科学, 2020, 48(24): 102-107.
	ZHANG J Y, JIANG J Z. Study on control efficacy of several fungicides on mango anthracnose by semi-fruit inoculation method[J]. Jiangsu Agricultural Sciences, 2020, 48(24): 102-107. (in Chinese)
[30]	林春花, 徐轩, 戈玉琪, 牟保辉, 刘文波, 缪卫国, 郑服丛. 中国2种橡胶树炭疽病菌对咪鲜胺敏感性比较[J]. 热带作物学报, 2017, 38(1): 111-115.
	LIN C H, XU X, GE Y Q, MOU B H, LIU W B, MIAO W G, ZHENG F C. Comparison of sensitivity between two Colletotrichum species from Hevea brasiliensis to prochloraz in China[J]. Chinese Journal of Tropical Crops, 2017, 38(1): 111-115. (in Chinese)
[31]	LYGIN A V, HILL C B, PAWLOWSKI M, ZERNOVA O V, WIDHOLM J M, HARTMAN G, LOZOVAYA V V. Inhibitory effects of stilbenes on the growth of three soybean pathogens in culture[J]. Phytopathology, 2014, 104(8): 843-850. DOI PMID
[32]	XU D, DENG Y, XI P, ZHU Z, KONG X, WAN L, SITU J, LI M, GAO L, JIANG Z. Biological activity of pterostilbene against Peronophythora litchii, the litchi downy blight pathogen[J]. Postharvest Biology and Technology, 2018, 144: 29-35.
[33]	黄小兰, 盖智星, 王日葵, 贺明阳, 韩冷, 周炼. 对羟基苯甲酸处理对采后柑橘炭疽病的抑制及机理研究[J]. 食品与机械, 2016, 32(9): 121-125, 208.
	HUANG X L, GAI Z X, WANG R K, HE M Y, HAN L, ZHOU L. Mechanism and inhibitory effect of p-hydrxybenzoic acid on anthracnose in postharvest citrus[J]. Food and Machinery, 2016, 32(9): 121-125, 208. (in Chinese)
[34]	杨婷, 史红安, 李聪丽, 李建华, 王立华, 李国元, 张志林. 13种萜类化合物对胶孢炭疽菌和链格孢的抑制活性[J]. 植物保护, 2017, 43(2): 192-195.
	YANG T, SHI H A, LI C L, LI J H, WANG L H, LI G Y, ZHANG Z L. Antifungal activity of 13 terpenoid compounds against Colletotrichum gloeosporioides and Alternaria sp[J]. Plant Protection, 2017, 43(2): 192-195. (in Chinese)
[35]	周丹丹, 王卓, 邢梦珂, 屠康, 周程雁. 植物精油抑制炭疽菌及对枇杷采后炭疽病与品质的影响[J]. 食品科学, 2017, 38(19): 212-217.
	ZHOU D D, WANG Z, XING M K, TU K, ZHOU C Y. Inhibitory effect of plant essential oils on colletorichum acutatum and postharvest anthracnose and quality of loquat fruits[J]. Food Science, 2017, 38(19): 212-217. (in Chinese)
[36]	王娅宁, 尉亚辉, 郝浩永, 姬婧媛. 白藜芦醇代谢物的研究进展[J]. 西北植物学报, 2007, 27(4): 4852-4857.
	WANG Y N, WEI Y H, HAO H Y, JI J Y. Advances in the research of resveratrol metabolins[J]. Acta Botanica Boreali-occidentalia Sinica, 2007, 27(4): 4852-4857. (in Chinese)
[37]	KOLOUCHOVÁ I, MAŤÁTKOVÁ O, PALDRYCHOVÁ M, KODEŠ Z, KVASNIČKOVÁ E, SIGLER K, ČEJKOVÁ A, ŠMIDRKAL J, DEMNEROVÁ K, MASÁK J. Resveratrol, pterostilbene, and baicalein: plant-derived anti-biofilm agents[J]. Folia Microbiologica, 2018, 63(3): 261-272. DOI PMID
[38]	PEZET R, PONT V. Ultrastructural observations of pterostilbene fungitoxicity in dormant conidia of Botrytis cinerea pers[J]. Journal of Phytopathology, 1990, 129(1): 19-30.

代谢物 Metabolite	浓度 Concentration /(μmol·L^-1)	孢子萌发抑制率Inhibition rate of spore germination/%
代谢物 Metabolite	浓度 Concentration /(μmol·L^-1)	柱花草胶孢炭疽 Stylo C. gloeosporioides	橡胶胶孢炭疽 Rubber C. gloeosporioides	橡胶尖孢炭疽菌 Rubber C. acutatum	芒果胶孢炭疽 Mango C. gloeosporioides	番木瓜胶孢炭疽 Papaya C. gloeosporioides
根皮素	1000	12.42±1.17^ef	0.91±1.86^c	9.16±2.42^f	-7.92±1.75^d	58.49±4.73^b
柚皮素	1000	13.42±2.93^ef	11.00±2.58^d	-3.97±3.95^h	6.77±3.32^c	17.84±3.07^e
香豆素	1000	16.56±3.89^de	0.88±0.66^c	1.13±0.64^gh	0.25±2.75^cd	50.40±5.17^bc
阿魏酸	1000	14.57±1.74^def	0.74±4.28^c	16.68±2.87^e	-12.86±1.87^f	35.95±5.62^cd
白藜芦醇	1000	47.50±4.75^b	-0.64±2.32^c	22.30±2.40^de	-3.05±4.69^cde	22.17±8.78^de
紫檀芪	1000	100^a	100^a	100^a	100^a	98.67±0.86^a
紫檀芪+根皮素	1000+1000	100^a	99.50±0.50^a	97.22±1.59^ab	100^a	99.73±0.27^a
紫檀芪+阿魏酸	1000+1000	100^a	91.89±1.99^b	89.52±2.71^c	100^a	100^a
紫檀芪+香豆素	1000+1000	100^a	91.53±1.32^b	91.20±1.39^bc	100^a	100^a
根皮素+香豆素	1000+1000	21.01±2.71^cd	9.59±1.11^d	-3.55±2.00^h	2.45±2.26^c	88.50±1.28^a
根皮素+阿魏酸	1000+1000	24.72±2.41^c	19.61±3.13^c	17.62±2.04^de	-8.35±2.54^d	51.17±8.51^bc
香豆素+阿魏酸	1000+1000	8.72±1.54^f	8.92±1.85^d	5.02±2.49^fg	20.47±4.19^b	51.55±9.91^bc
白藜芦醇+柚皮素	1000+1000	46.93±2.01^b	7.84±4.03^d	24.35±2.84^d	16.27±3.67^b	43.53±10.36^bc

代谢物 Metabolite	浓度 Concentration /(μmol·L^-1)	孢子萌发抑制率Inhibition rate of spore germination/%
代谢物 Metabolite	浓度 Concentration /(μmol·L^-1)	柱花草胶孢炭疽 Stylo C. gloeosporioides	橡胶胶孢炭疽 Rubber C. gloeosporioides	橡胶尖孢炭疽菌 Rubber C. acutatum	芒果胶孢炭疽 Mango C. gloeosporioides	番木瓜胶孢炭疽 Papaya C. gloeosporioides
根皮素	1000	12.42±1.17^ef	0.91±1.86^c	9.16±2.42^f	-7.92±1.75^d	58.49±4.73^b
柚皮素	1000	13.42±2.93^ef	11.00±2.58^d	-3.97±3.95^h	6.77±3.32^c	17.84±3.07^e
香豆素	1000	16.56±3.89^de	0.88±0.66^c	1.13±0.64^gh	0.25±2.75^cd	50.40±5.17^bc
阿魏酸	1000	14.57±1.74^def	0.74±4.28^c	16.68±2.87^e	-12.86±1.87^f	35.95±5.62^cd
白藜芦醇	1000	47.50±4.75^b	-0.64±2.32^c	22.30±2.40^de	-3.05±4.69^cde	22.17±8.78^de
紫檀芪	1000	100^a	100^a	100^a	100^a	98.67±0.86^a
紫檀芪+根皮素	1000+1000	100^a	99.50±0.50^a	97.22±1.59^ab	100^a	99.73±0.27^a
紫檀芪+阿魏酸	1000+1000	100^a	91.89±1.99^b	89.52±2.71^c	100^a	100^a
紫檀芪+香豆素	1000+1000	100^a	91.53±1.32^b	91.20±1.39^bc	100^a	100^a
根皮素+香豆素	1000+1000	21.01±2.71^cd	9.59±1.11^d	-3.55±2.00^h	2.45±2.26^c	88.50±1.28^a
根皮素+阿魏酸	1000+1000	24.72±2.41^c	19.61±3.13^c	17.62±2.04^de	-8.35±2.54^d	51.17±8.51^bc
香豆素+阿魏酸	1000+1000	8.72±1.54^f	8.92±1.85^d	5.02±2.49^fg	20.47±4.19^b	51.55±9.91^bc
白藜芦醇+柚皮素	1000+1000	46.93±2.01^b	7.84±4.03^d	24.35±2.84^d	16.27±3.67^b	43.53±10.36^bc

代谢物Metabolite	菌株Strain	回归方程Regression equation	半抑制浓度IC₅₀/(μmol·L^-1)	相关系数R²
紫檀芪	柱花草胶孢炭疽菌	y=8.531x-22.396	422.046	0.926
	橡胶胶孢炭疽菌	y=4.00x-8.78	158.090	0.998
	橡胶尖孢炭疽菌	y=5.473x-13.203	258.325	0.992
	芒果胶孢炭疽菌	y=2.517x-5.345	132.884	0.993
	番木瓜胶孢炭疽菌	y=3.043x-5.584	68.417	0.956
紫檀芪+根皮素	柱花草胶孢炭疽菌	y=2.488x-6.140	293.475	0.960
	橡胶胶孢炭疽菌	y=4.108x-9.092	163.332	0.962
	橡胶尖孢炭疽菌	y=9.073x-22.859	330.770	0.972
	芒果胶孢炭疽菌	y=8.649x-17.609	108.671	0.994
	番木瓜胶孢炭疽菌	y=5.703x-11.000	84.905	0.931
紫檀芪+阿魏酸	柱花草胶孢炭疽菌	y=2.846x-7.828	536.712	0.952
	橡胶胶孢炭疽菌	y=1.357x-2.483	67.660	0.999
	橡胶尖孢炭疽菌	y=4.947x-11.213	184.764	0.868
	芒果胶孢炭疽菌	y=2.090x-5.480	418.270	0.850
	番木瓜胶孢炭疽菌	y=7.014x-16.344	213.844	0.963
紫檀芪+香豆素	柱花草胶孢炭疽菌	y=5.182x-13.990	500.808	0.961
	橡胶胶孢炭疽菌	y=3.238x-7.081	153.755	0.945
	橡胶尖孢炭疽菌	y=4.593x-11.767	364.661	0.975
	芒果胶孢炭疽菌	y=5.499x-12.163	162.851	0.967
	番木瓜胶孢炭疽菌	y=4.029x-7.808	86.662	0.953

代谢物Metabolite	菌株Strain	回归方程Regression equation	半抑制浓度IC₅₀/(μmol·L^-1)	相关系数R²
紫檀芪	柱花草胶孢炭疽菌	y=8.531x-22.396	422.046	0.926
	橡胶胶孢炭疽菌	y=4.00x-8.78	158.090	0.998
	橡胶尖孢炭疽菌	y=5.473x-13.203	258.325	0.992
	芒果胶孢炭疽菌	y=2.517x-5.345	132.884	0.993
	番木瓜胶孢炭疽菌	y=3.043x-5.584	68.417	0.956
紫檀芪+根皮素	柱花草胶孢炭疽菌	y=2.488x-6.140	293.475	0.960
	橡胶胶孢炭疽菌	y=4.108x-9.092	163.332	0.962
	橡胶尖孢炭疽菌	y=9.073x-22.859	330.770	0.972
	芒果胶孢炭疽菌	y=8.649x-17.609	108.671	0.994
	番木瓜胶孢炭疽菌	y=5.703x-11.000	84.905	0.931
紫檀芪+阿魏酸	柱花草胶孢炭疽菌	y=2.846x-7.828	536.712	0.952
	橡胶胶孢炭疽菌	y=1.357x-2.483	67.660	0.999
	橡胶尖孢炭疽菌	y=4.947x-11.213	184.764	0.868
	芒果胶孢炭疽菌	y=2.090x-5.480	418.270	0.850
	番木瓜胶孢炭疽菌	y=7.014x-16.344	213.844	0.963
紫檀芪+香豆素	柱花草胶孢炭疽菌	y=5.182x-13.990	500.808	0.961
	橡胶胶孢炭疽菌	y=3.238x-7.081	153.755	0.945
	橡胶尖孢炭疽菌	y=4.593x-11.767	364.661	0.975
	芒果胶孢炭疽菌	y=5.499x-12.163	162.851	0.967
	番木瓜胶孢炭疽菌	y=4.029x-7.808	86.662	0.953