Physiological and Biochemical Analysis of <i>Oncidium </i>in Response to Stress of Soft Rot Pathogen
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1610-1615.DOI: 10.3969/j.issn.1000-2561.2020.08.015

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Physiological and Biochemical Analysis of Oncidium in Response to Stress of Soft Rot Pathogen

WU Qiuzhen,LIN Zhengchun,NI Shanshan,LAI Zhongxiong()   

  1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2019-11-12 Revised:2019-11-28 Online:2020-08-25 Published:2020-09-17
  • Contact: LAI Zhongxiong


The pseudobulb of Sherry Baby, Oncidium was infected by bacterial soft rot bacteria infection. The first leaf on the pseudobulb was sampled 0, 8, 16, 24, 32 h, after infection to determine the chlorophyll fluorescence properties [Fv/Fm, Y(Ⅱ), Y(NPQ), Y(NO)], superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO) activity, malondialdehyde (MDA), lignin, and the change of soluble sugar content. The results showed that with the extension of infection time, Fv/Fm, Y(Ⅱ), Y(NPQ) gradually decline, Y(NO) increased, and the photosynthetic system was damaged. PPO activity and lignin content decreased gradually; SOD and POD activity increased gradually; CAT activity and soluble sugar content first increased and then decreased; MDA content decreased gradually from 0 to 24 h and then leveled off after 24 h. The study would heip to explore the resistance mechanism and establish the physiological method of resistance identification.

Key words: Oncidium spp., soft rot, physiological and biochemical indexes, chlorophyll fluorescence properties, lignin

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