Abstract:

In order tooptimize the chromosome mounting technique and explore the chromosome karyotype of C. kanehirae Hay, and to provide important cytological evidences for the study of the evolution, evolutionary characteristics and genetic regularity of camphor plants, the effects of differentpretreatment methods and enzymatic hydrolysis time on the production of C. kanehirae were discussed by the enzymatic dissociation wall low permeability method. The observation showed that pretreated in 0.002 mol/L 8-hydroxyl for 2 h, dissociated in 4% cellulase and 5% pectinase for 5 h could achieve the optimal experimental results. The karyotype analysis showed that the chromosome number was 24, with a karyotype formula 2n=2x=24=22m+2sm. The results demonstrated that the asymmetry index was 55.81%, the karyotype was 2B type, suggesting that C. kanehirae Hay maybe a relatively primitive species.

Key words: Cinnamomum kanehirae Hay of sharp leaves, root tip, chromosome preparation, karyotype analysis