Analysis on Enzyme Activity and Microbial Community Diversity in Rhizosphere Soil of Different Sugarcane Varieties
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 819-828.DOI: 10.3969/j.issn.1000-2561.2020.04.025

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Analysis on Enzyme Activity and Microbial Community Diversity in Rhizosphere Soil of Different Sugarcane Varieties

NONG Zemei1,2,SHI Guoying1,ZENG Quan1,YE Xuelian1,QIN Huadong2,*(),HU Chunjin1,*()   

  1. ⒈ Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
    2. College of Agriculture, Guangxi University, Nanning, Guangxi 530005, China
  • Received:2019-03-27 Revised:2019-09-09 Online:2020-04-25 Published:2020-05-09
  • Contact: QIN Huadong,HU Chunjin


In order to provide scientific support for the selection of sugarcane production and the maintenance of sugarcane soil productivity, the differences in nitrogen uptake efficiency of eight sugarcane varieties and the effects of different varieties on the enzyme activities and microbial community diversity in the rhizosphere soil were compared through the barrel cultivation experiment. Sugarcane plants and rhizosphere soils were collected at the end of large growth stage of sugarcane, and nitrogen utilization efficiency, invertase and urease activities in the rhizosphere soil of different sugarcane varieties were measured and compared. Meanwhile, the bacterial community structure of the sugarcane rhizosphere soil was determined and analyzed by the Miseq high-throughput sequencing technology. The nitrogen uptake efficiency of different sugarcane varieties was different. The average nitrogen uptake efficiency of the eight varieties was 17.95%-27.57%, and the nitrogen use efficiency was 22.15%-34.02%. The highest nitrogen uptake efficiency was for GT44, and the lowest was for B9. The invertase activity of the rhizosphere soil was 3.51-6.56 mg/(g·d), and there were significant differences among different cultivars. But the urease activity was 1.12-1.42 mg/(g·d), and there was few differences among different varieties. GT48 and ROC22 were the two varieties with higher soil enzyme activity in the rhizosphere. The results of high-throughput sequencing showed that there were some differences in the bacterial population structure in the rhizosphere soil of the eight sugarcane varieties, but there was no significant difference among different varieties in the composition of the dominant bacteria, and the main difference was in the abundance of the dominant genera. Bacillus was the dominant genus for GT44 and Y93159, while Chryseolinea was the dominant genus for the other six varieties. Redundancy analysis (RDA) showed that the nitrogen absorption efficiency of sugarcane, the content of available N and pH value of soil had the greatest effect on the soil microbial community structure. Correlation analysis showed that the nitrogen absorption efficiency of sugarcane and soil physiochemical characteristics were significantly correlated with different dominant microbial communities. This study would deepen the understanding of the microbial community in the sugarcane rhizosphere and provide references for the relation between the microbial composition and diversity with environmental factors.

Key words: sugarcane, rhizosphere soil, soil enzyme activity, microbial community diversity

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