Cloning of Mango Gene <i>MinMYB10</i> and Vector Construction for Expression Analysis
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (7): 1297-1303.DOI: 10.3969/j.issn.1000-2561.2019.07.008

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Cloning of Mango Gene MinMYB10 and Vector Construction for Expression Analysis

BAI Beibei1,2,JING Yonglin1,2,LAN Li1,WANG Jia1,ZHAO Zhichang2,*()   

  1. 1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
  • Received:2018-08-01 Revised:2019-03-06 Online:2019-07-25 Published:2019-08-01
  • Contact: ZHAO Zhichang


The MYB (myeloblastosis) transcription factors are mainly involved in the regulation of flavonoid metabolism, growth and development in plants. In order to study how the flavonoid metabolic pathways are regulated in mango fruit, a MYB gene, designated as MinMYB10, was cloned from the flesh of Jinhuang mango fruit. The full-length cDNA sequence of MinMYB10 was 1021 bp long, containing an open reading frame of 837 bp which would encode a peptide of 278 amino acid residues with a molecular weight of 31.60 ku and an isoelectric point of 5.89. Phylogenetic analysis showed that the encoded protein sequence was closely related to its homolog from Arabidopsis thaliana. We have also constructed a silencing vector, pTRV2-MinMYB10, and an over expression vector, pGreenII 62-SK-MinMYB10, aiming to decipher the role of MinMYB10 in the metabolism of anthocyanin in mango fruit in our upcoming researches.

Key words: mango, MinMYB10 gene, vector construction

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