Analysis on Volatiles of Oolong Tea Varieties Induced by <i>Empoasca</i> sp.
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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (3): 576-582.DOI: 10.3969/j.issn.1000-2561.2019.03.023

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Analysis on Volatiles of Oolong Tea Varieties Induced by Empoasca sp.

JIN Shan1,2,HAN Liwei2,YE Naixing1,WANG Wei1,HUANG Huoshui3,*(),LIU Wei1,4,5,*()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University / Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, Fujian 350002, China
    2. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    3. Integrated Technical Service Center, Quanzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Quanzhou, Fujian 362000, China
    4. College of Life Sciences, Ningde Normal University, Ningde, Fujian 352000, China
    5. Collaborative Innovation Center of Chinese Oolong Tea Industry, Wuyi University, Wuyishan, Fujian 354300, China
  • Received:2018-08-01 Revised:2018-11-15 Online:2019-03-15 Published:2019-03-15
  • Contact: HUANG Huoshui,LIU Wei


The changes of tea plant volatiles induced by tea green leafhopper Empoasca sp. were determined to provide basis for breeding of resistant tea varieties. Based on the field observation of Empoasca sp. on different tea varieties from Fujian province, Rougui and Tieguanyin were found to have the highest population density and the lowest population density of Empoasca sp. respectively, and selected as the research materials in this paper. Gas chromatography-mass spectrometer (GC-MS) was used to determine and analyze constitutive volatiles of tea and induced volatiles by Empoasca sp. The results showed that P-Cymene, beta.-Ocimene, Trans.-beta.-Ocimene, alpha.-Farnesene, gamma.-Terpineneand and 3-Methyl-3-cyclohexen-1-one were the main constituent volatiles of Tieguanyin and Rougui. The 1,6-Octadien-3-ol, 3,7-dimethyl- was detected only in the health shoots of Tieguanyin, while Tridecane, Acetic acid octyl ester, Hexadecane and Cedrol were be detected only in Rougui. The content of beta.-Myrcene, beta.-Ocimene, Trans.-beta.-Ocimene, alpha.-Farnesene and 1,6-Octadien-3-ol, 3,7-dimethyl were far higher than that of Rougui. In combination with other literature, it is inferred that these volatile chemicals possibly resulted in the significant difference in the population density of Empoasca sp. between Tieguanyin and Rougui. After a period of damage by Empoasca sp., the composition and content of volatiles in Tieguanyin and Rougui changed greatly. Both of them released large amounts of beta.-Myrcene, beta.-Ocimene, Trans.-beta.-Ocimene and alpha.-Farnesene, the contents of these chemicals largely increased in 4 h and 8 h damage treatments with Empoasca sp. ranged from 1.49 to 41.22 times than them released from health tea shoots. In addition, Empoasca sp. feeding also induced new volatile chemical including (Z)-Butanoic acid 3-hexenyl ester, Decanal, Indole, Hexanoicacid 3-hexenyl ester, and phenylethyl alcohol. The 10 volatile chemicals mentioned above may be related to the induced resistance of tea plant and the resistance of Tieguanyin against to Empoasca sp.

Key words: Camellia sinensis (L.) O. Ktze., Empoasca sp., volatile components, resistance