Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (11): 2255-2260.DOI: 10.3969/j.issn.1000-2561.2019.11.021

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Optimization of Extraction Process and Detection Method of Dencichine

DUAN Shaofeng1,2,3,CHEN Geng1,2,3,YAN Jing1,2,3,QIN Zhongming3,ZHANG Guanghui1,2,LIANG Yanli1,2,3,*()   

  1. 1. National & Local Joint Engineering Research Center on Germplasms Utilization & Innovation of Chinese Medicinal Materials in Southwest, Yunnan Agricultural University, Kunming, Yunnan 650201, China
    2. Yunnan Provincial Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
    3. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
  • Received:2019-02-19 Revised:2019-05-14 Online:2019-11-25 Published:2019-11-28
  • Contact: Yanli LIANG


The extraction process of dencichine in Panax notoginseng was optimized by the L9(3 4) orthogonal test, and the content of dencichine was determined by HPLC. The optimized extraction condition for dencichine was extracted once with ultrapure water at solid-to-liquid ratio of 1∶20 (g/mL) for 10 min. Flower bud had the highest dencichine content, followed by rhizome, main roots and leaves. The content of dencichine in flower buds was 3.33%. There was no significant correlation between the content of dencichine and the rhizom size. The content of dencichine in the rhizome of P. zingiberensis and P. vienamensis var. fuscidiscus was significantly lower than that of the main root of P. notoginseng. It was also significantly lower than that of other parts of P. notoginseng, indicating that P. notoginseng was the best source of dencichine. The content of dencichine was mainly affected by species and parts, and had nothing to do with product specifications. The extraction method and HPLC detection method established in this paper could quickly and easily determine the content of dencichine. It can lay a foundation for the establishment of related standards of dencichine and provide a basis for the comprehensive utilization and development of P. notoginseng.

Key words: Panax notoginseng, dencichine, HPLC, supersonic extraction