Optimization of Extraction Process and Detection Method of Dencichine

doi:10.3969/j.issn.1000-2561.2019.11.021

Abstract

Abstract:

The extraction process of dencichine in Panax notoginseng was optimized by the L₉(3 ⁴) orthogonal test, and the content of dencichine was determined by HPLC. The optimized extraction condition for dencichine was extracted once with ultrapure water at solid-to-liquid ratio of 1∶20 (g/mL) for 10 min. Flower bud had the highest dencichine content, followed by rhizome, main roots and leaves. The content of dencichine in flower buds was 3.33%. There was no significant correlation between the content of dencichine and the rhizom size. The content of dencichine in the rhizome of P. zingiberensis and P. vienamensis var. fuscidiscus was significantly lower than that of the main root of P. notoginseng. It was also significantly lower than that of other parts of P. notoginseng, indicating that P. notoginseng was the best source of dencichine. The content of dencichine was mainly affected by species and parts, and had nothing to do with product specifications. The extraction method and HPLC detection method established in this paper could quickly and easily determine the content of dencichine. It can lay a foundation for the establishment of related standards of dencichine and provide a basis for the comprehensive utilization and development of P. notoginseng.

Key words: Panax notoginseng, dencichine, HPLC, supersonic extraction

DUAN Shaofeng,CHEN Geng,YAN Jing,QIN Zhongming,ZHANG Guanghui,LIANG Yanli. Optimization of Extraction Process and Detection Method of Dencichine[J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2255-2260.

Figures/Tables 7

References 22

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水平 Level	（见表2）：因素Factor
水平 Level	A：提取次数 Extraction times	B：甲醇体积分数 Methanol volume fraction/%	C：提取时间 Ultrasonic time/min	D：料液比 Solid-to-liquid ratio/(g·mL^-1)
1	1	0	10	1:20
2	2	50	20	1:30
3	3	100	30	1:50

水平 Level	（见表2）：因素Factor
水平 Level	A：提取次数 Extraction times	B：甲醇体积分数 Methanol volume fraction/%	C：提取时间 Ultrasonic time/min	D：料液比 Solid-to-liquid ratio/(g·mL^-1)
1	1	0	10	1:20
2	2	50	20	1:30
3	3	100	30	1:50

编号 No.	因素Factor				总提取量 The total extract quantity/%
编号 No.	A：提取次数 Extraction times	B：甲醇体积分数 Methanol volume fraction/%	C：提取时间 Ultrasonic time/min	D：料液比 Solid-to-liquid ratio (g:mL^-1)	总提取量 The total extract quantity/%
1	1	0	10	1:20	1.92
2	1	0	20	1:30	1.23
3	1	0	30	1:50	0.98
4	2	50	20	1:50	1.02
5	2	50	30	1:20	1.49
6	2	50	10	1:30	1.12
7	3	100	30	1:30	0.71
8	3	100	10	1:50	0.58
9	3	100	20	1:20	0.69
K₁	1.09	1.28	1.09	1.59
K₂	1.11	1.15	1.04	1.01
K₃	0.94	0.32	0.91	0.68
R	0.17	0.96	0.18	0.91

编号 No.	因素Factor				总提取量 The total extract quantity/%
编号 No.	A：提取次数 Extraction times	B：甲醇体积分数 Methanol volume fraction/%	C：提取时间 Ultrasonic time/min	D：料液比 Solid-to-liquid ratio (g:mL^-1)	总提取量 The total extract quantity/%
1	1	0	10	1:20	1.92
2	1	0	20	1:30	1.23
3	1	0	30	1:50	0.98
4	2	50	20	1:50	1.02
5	2	50	30	1:20	1.49
6	2	50	10	1:30	1.12
7	3	100	30	1:30	0.71
8	3	100	10	1:50	0.58
9	3	100	20	1:20	0.69
K₁	1.09	1.28	1.09	1.59
K₂	1.11	1.15	1.04	1.01
K₃	0.94	0.32	0.91	0.68
R	0.17	0.96	0.18	0.91

头数 Quantity	三七素含量 Dencichine content/%	商品 Commodity	三七素含量 Dencichine content/%
20	1.24±0.0942^a	三七花 Panax notoginseng flowers	3.33±0.3360^a
30	1.18±0.1764^ab	筋条Rootlet	1.32±0.0786^ab
40	1.02±0.0752^abc	剪口Rhizome	1.57±0.0686^a
45	1.23±0.1388^a	须根Fibrous root	1.22±0.4485^ab
50	1.18±0.0127^ab
60	0.97±0.0216^bc