Tissue Culture and Rapid Propagation Technology of Hopson Flower of Musa velutina

doi:10.3969/j.issn.1000-2561.2019.11.011

Abstract

Abstract:

As a wild banana resource, Musa velutina can be regarded as an important genetic and breeding resource and has an ornamental value. In this study, the tissue culture and rapid propagation technology of Musa velutina were studied using the hopson flowers as the plant materials. Callus could be induced by the callus induction medium. The best bud differentiation medium was MS+6-BA (3.0 mg/L)+NAA (0.1-0.2 mg/L), the best bud proliferation medium was MS+6-BA (2.0 mg/L)+NAA (0.1 mg/L), and the best rooting medium was MS+IBA (3.0 mg/L)+NAA (0.3 mg/L). It took about 100 d from sampling to obtaining tissue culture seedlings with complete root system, and the reproduction efficiency was more than 100 plants/flower bud.

Key words: Musa velutina, hopson flowers, tissue culture, bud differentiation, bud proliferation

HUANG Dongmei,XU Yi,LI Jingyang,LI Yujia,LIN Fei,LI Yanxia,SONG Shun. Tissue Culture and Rapid Propagation Technology of Hopson Flower of Musa velutina[J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2174-2177.

Figures/Tables 3

References 18

[1]	Häkkinen M, Sharrock S . Diversity in the genus Musa-focus on Rhodochlamys[J]. INIBAP Annual Report, 2001,8:16-23.
[2]	Häkkinen M . Ornamental bananas: focus on Rhodochlamys[J]. Folia Malaysiana, 2005,6:49-72.
[3]	Häkkinen M . Musa chunii Häkkinen, a new species (Musaceae) from Yunnan, China and taxonomic identity of Musa rubra[J]. Journal of Systematics and Evolution, 2009,47(1):87-91.
[4]	Sabu M, Joe A, Sreejith P E . Musa velutina subsp. markkuana (Musaceae): a new subspecies from northeastern India[J]. Phytotaxa, 2013,92(2):49-54.
[5]	Simmonds N W, Shepherd K . The taxonomy and origins of the cultivated bananas[J]. Journal of the Linnean Society of London Botany, 1955,55:302-312.
[6]	Joe A, Sabu M, Sreejith P E . A new variety ofMusa velutina H. Wendl. & Drude (Musaceae) from Assam, North-East India[J]. Plant Systematics and Evolution, 2014,300(1):13-17.
[7]	冯慧敏, 陈友, 邓长娟 , 等. 芭蕉属野生种的地理分布[J]. 果树学报, 2009,26(3):361-368.
[8]	李锡文 . 云南芭蕉科植物[J]. 植物分类学报, 1978,16(3):54-64.
[9]	Häkkinen M, Väre H . Taxonomic history and identity of Musa dasycarpa, M. velutina and M. assamica (Musaceae) in Southeast Asia[J]. Journal of Systematics and Evolution, 2008,46(2):230-235.
[10]	张光勇, 陈伟强, 刘学敏 , 等. 云南省野生香蕉资源收集及保存[J]. 热带农业科技, 2011,34(1):36-38.
[11]	Uma S, Saraswathi M S, Durai P , et al. Diversity and distribution of section Rhodochlamys (Genus Musa, Musaceae) in India and breeding potential for banana improvement programmes[J]. Plant Genetic Resources Newsletter, 2006,146:17-23.
[12]	Capdeville G D, Júnior M T S, Szinay D , et al. The potential of high-resolution BAC-FISH in banana breeding[J]. Euphytica, 2009,166(3):431-443.
[13]	Kirchoff B K . Inflorescence and flower development in Musa velutina H. Wendl. & Drude (Musaceae), with a consideration of developmental variability, restricted phyllotactic direction, and hand initiation[J]. International Journal of the Plant Sciences, 2017,178:259-272.
[14]	刘伟良, 陈友, 王静毅 , 等. 云南热带地区野生香蕉资源考察及分布现状分析[J]. 热带农业科学, 2007,27(3):31-34.
[15]	Wallace R, Häkkinen M . Musa × georgiana, a new intersectional hybrid banana with edible banana breeding relevance and ornamental potential[J]. Nordic Journal of Botany, 2010,27(3):182-185.
[16]	Häkkinen M . Ornamental bananas: focus on Rhodochlamys[J]. Chronica Horticulturae, 2007,47:7-12.
[17]	张建斌, 贾彩红, 刘菊华 , 等. 香蕉未成熟雄花组织培养与快速繁殖研究[J]. 热带作物学报, 2012,33(7):1225-1229.
[18]	Pancholi N, Wetten A, Caligari P D S . Germination of Musa velutina seeds: Comparison of in vivo and in vitro systems[J]. Vitro Cellular & Development Biology Plant, 1995,31(3):127-130.