In order to understand the mineral element content of different cassava germplasm resource and to select the germplasm with rich content, and provide data support for the comprehensive utilization of cassava, the contents of ash and mineral elements in 113 cassava germplasm resources were determined by muffle furnace method, atomic absorption spectrometry and atomic fluorescence spectrometry. The results showed that the content of mineral element of Cr, Cu and Se was lower than 1 mg/kg (dry weight), and the content of Ca, Fe, K, Mg, Mn, Na and Zn was 66.39-1609.81 mg/kg, 4.35-38.93 mg/kg, 1954.56-8762.78 mg/kg, 149.04-1143.73 mg/kg, 4.48-37.40 mg/kg, 36.80-530.40 mg/kg, and 6.35- 24.83 mg/kg, respectively. The ash content and mineral element content of the 113 cassava varieties were divided into five grades by the probability grading method, namely extremely high, high, medium, low and very low. Extremely high strains were recommended as basic parent materials. In the correlation analysis, ash content had a significant correlation with Fe, Mg, Na and Zn content, Ca content had a very significant positive correlation with K and Mn content, Ca content had a very significant negative correlation with Mg and Na content, Fe content had a very significant positive correlation with Mg, Na and Zn content, K content had a very significant positive correlation with Mn content, a very significant negative correlation with Na content, Mg content had a very significant negative correlation with Na and Zn, and Zn content had a very significant positive correlation with Mn and Na content. Therefore, the absorption of Zn and various mineral elements in cassava germplasm resources had a strong mutual promotion, Fe content and Mg, Na content had a strong mutual promotion, Ca content and K, Mn content had a strong promotion, while the absorption of Ca, K, Mn and Fe, Mg, Na content may have a certain antagonistic effect. Further combined with principal component analysis and cluster analysis, the mineral element contents of different cassava varieties were comprehensively evaluated, and two principal components with eigenvalues greater than 1 was obtained, with a cumulative variance contribution rate of 61.707%. Cluster analysis could divide the 113 cassava varieties into three categories. According to the difference of mineral element contents, different cassava varieties could be divided into high Na, Mg, Zn, Fe content group and high Mn, Ca, K, Zn content group. Cassava varieties such as ‘274' ‘50' ‘571' ‘521' ‘417' had higher scores in the comprehensive evaluation of principal components.