Genetic Diversity Analysis and Molecular ID Construction of Mango Germplasm Based on SSR Fluorescence Markers
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2292-2304.DOI: 10.3969/j.issn.1000-2561.2023.11.018

• Germplasm Resources, Genetics & Breeding • Previous Articles     Next Articles

Genetic Diversity Analysis and Molecular ID Construction of Mango Germplasm Based on SSR Fluorescence Markers

TANG Yujuan1,2, LUO Shixing1,2, HUANG Guodi1,2,*(), SONG Enliang1, LI Riwang1,2, ZHAO Ying1,2, ZHANG Yu1,2, MO Yonglong1,2, TANG Yingying1,2   

  1. 1. Guangxi Subtropical Crops Research Institute, Nanning, Guangxi 530001, China
    2. Guangxi Zhuang Autonomous Region Engineering Research Center of Green and Efficient Development for Mango Industry, Nanning, Guangxi 530001, China
  • Received:2022-09-08 Revised:2022-10-24 Online:2023-11-25 Published:2023-12-08
  • Contact: *HUANG Guodi,E-mail:1161737795@qq.com

Abstract:

Abundant germplasm resources are the basis for mango variety breeding and industrial development. For better protection and utilization of mango germplasm resources, the TP-M13-SSR marker developed by our team previously were used to analyze the genetic diversity and construct molecular ID of 145 mango germplasms containing local cultivars, bred varieties and wild relative species, which stored in the nursery of Guangxi Innovation Base of mango germplasm resources conservation. The results showed that the average number of observed alleles for the 12 primer pairs was 3.2838, the average observed heterozygosity (Ho) was 0.5858, the average expected heterozygosity (He) was 0.6725, the average Shannon index (I) was 1.3383, and the average Nei’s gene diversity index (Na) was 0.6702. The polymorphism information content (PIC) of the 12 primer pairs ranged from 0.5036 to 0.7827, with an average value of 0.6396. All the primers were highly polymorphic sites. The result suggested that TP-M13-SSR primer could provide data support for genetic diversity analysis of mango. The genetic similarity coefficient of 145 materrials varied from 0.5676 to 1.000, with an average of 0.7417. The genetic similarity coefficient between Irwin and Indian No. 1 was 1.000. The minimum genetic similarity coefficient was 0.5676, between M. persiciformis 20-2 and Dadouxiang mango, M. persiciformis 20-2 and Shuoshuai mango, Jinhuang mango and Guire 10-1 mango. All the 145 mango germplasms were divided into two groups when the genetic similarity coefficient was 0.7060. Group I contained 108 mango species and 20 M. persiciformis species, accounting for 88.90% of the total number of germplasms. Group Ⅱ contained 17 specimens, all of which were M. persiciformis species. Group I could be further divided into five subgroups when the genetic similarity coefficient was 0.7330, among which subgroups I-1 and I-3 were the most abundant, accounting for 91.92% of all mango germplasms. The results of UPGMA clustering analysis showed that M. persiciformis were not clustered strictly according to the species relationship, and the overall clustering result of mango germplasms was basically consistent with its geographical origin. All the 145 materials were amplified by 12 pairs of SSR fluorescent primers to obtain the fingerprint map, and the molecular ID was obtained by the assignment of numbers and letters combination. Each pair of primers could distinguish 12.4 germplasms on average, and the identification rate was significantly higher than that of previous studies, indicating that TP-M13-SSR had more advantageous than denaturing polyacrylamide gel electrophoresis in mango germplasm identification. This study would provide scientific basis for the collection and utilization of germplasm resources and variety breeding of mango. It is also proposed for molecular identification of bred varieties, which is of great significance to the development of mango industry for providing methods for molecular identification and intellectual property of bred varieties.

Key words: Mangifera indica L., TP-M13-SSR, genetic diversity, molecular identity

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