Establishment and Application of a Method for Genome Size Estimation of <i>Piper</i> L. Using Flow Cytometry
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2265-2272.DOI: 10.3969/j.issn.1000-2561.2023.11.015

• Omics & Biotechnology • Previous Articles     Next Articles

Establishment and Application of a Method for Genome Size Estimation of Piper L. Using Flow Cytometry

WU Baoduo1,3,4, HU Lisong1,3,4, FENG Jianxiong2, FAN Rui1,3,4, GUO Fen2, JI Xunzhi1,3,4, HAO Chaoyun1,3,4, YAN Lin1,4,*()   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
    2. College of Tropical Crop Science, Yunnan Agricultural University, Pu’er, Yunnan 665000, China
    3. Hainan Sim Soonliang Academician Workstation, Wanning, Hainan 571533, China
    4. Key Laboratory of Genetic Resources Utilization of Spiced Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
  • Received:2022-10-10 Revised:2022-11-16 Online:2023-11-25 Published:2023-12-08
  • Contact: *YAN Lin,E-mail:yanlin2575@163.com

Abstract:

Six nuclear extracts and three leaves of different development stage were screened to establish the flow cytometry (FCM) method for determining the genomic size of Piper L. and Cucumis sativus L. was used as the DNA reference standard. Result showed that the modified nuclear extract and inverted leaf were suitable for the flow cytometry of Piper L. The genome size of cultivated varieties, including Kuching, Aman and EMAS, ranged from 667.94 to 719.32 Mb, and Reyin1, microphylla variety from Cambodia, Panniyur-1 and 73F5 ranged from 759.69 to 785.38 Mb. Therefore, the genome size was significantly different among cultivated varieties. Furthermore, it is speculated that the chromosome numbers of P. wallichii and P. hancei, with genome size larger than 900 Mb, should be larger than 52 with a multiple of 13, and the number of chromosomes of germplasm, with genome size ranged from 600 to 800 Mb, may be 52. The hybrid not only had similar phenotypes to cultivated varieties and P. flaviflorum, but also had an average genome size of cultivated varieties and P. flaviflorum. Therefore, it is speculated that the hybrid may be the hybrid offspring of cultivated varieties and P. flaviflorum. This study would provide a method for the determination of the genome size of Piper L. germplasm, and provide a technical basis for the study of Piper L. germplasm evaluation and distant hybridization.

Key words: Piper L., flow cytometry, modified nuclear extract, genomic size

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