Identification and Analysis of ACA<i>/</i>ECA Gene Family in <i>Hevea brasiliensis</i>
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2244-2255.DOI: 10.3969/j.issn.1000-2561.2023.11.013

• Omics & Biotechnology • Previous Articles     Next Articles

Identification and Analysis of ACA/ECA Gene Family in Hevea brasiliensis

YANG Jingjing1,2, FANG Yongjun1, ZHANG Hongtao1,3, LONG Xiangyu1, QIN Yunxia1, YANG Jianghua1, XIAO Xiaohu1,*()   

  1. 1. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2. College of Tropical Crops, Yunnan University, Pu’er, Yunnan 665099, China
    3. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
  • Received:2022-10-21 Revised:2022-11-09 Online:2023-11-25 Published:2023-12-08
  • Contact: *XIAO Xiaohu,E-mail:xiaofeihu_011588@126.com

Abstract:

Ca2+ is a second messenger in plant cells, and Ca2+-ATPase (ACA/ECA), as an important protein in Ca2+ transport, plays a crucial role in ensuring the balance of intracellular calcium ions and abiotic stress in plants. To investigate the biological function of Ca2+-ATPase (ACA/ECA) in Hevea brasiliensis, 45 ACA/ECA gene family members were identified from the rubber tree genome using the ACA/ECA protein sequence of the model plant Arabidopsis as a probe, including 38 ACA members and 7 ECA members. Expasy and Plant-mPLoc were used to comprehensively analyze the physicochemical properties, gene structure, chromosomal localization, phylogeny, and expression patterns of the 45 members. Physicochemical properties analysis showed that the number of amino acids encoded by each member was 86-1142, the molecular weight of amino acids ranged from 10 048.04 to 125 412.75 Da, and the protein products were mostly localized on the cytoplasmic membrane, with a few members localized on the endoplasmic reticulum, chloroplast, vacuole, and nucleus. In evolutionary, members of the ACA/ECA family were clearly clustered into ACA and ECA two branches, and rubber tree members were always in close proximity to cassava in both branches, indicating that there was a close relative between rubber tree and cassava during phylogeny. Chromosomal localization revealed that the 45 members of the ACA/ECA family were unevenly distributed across 14 chromosomes and one contig in rubber tree, in which, chromosomes 2 and 9 had the largest member distribution of 11 members. Expression analysis showed that there were obvious differences in the expression of some members in different groups, HbACA31 was expressed the most in leaves, HbACA36 was expressed the most in latex, and the expression of HbACA36 was obviously up-regulated in latex after treatment with ethenol. In addition, we also analyzed the influence of tapping on ACA/ECA gene expression, and found that HbACA36 was at a high expression level during tapping of the two rubber tree varieties, PR107 and Reyan 8-79, and significantly up-regulated during tapping of high-yield variety Reyan 8-79,which speculated that HbACA36 was involved in the regulation of rubber biosynthesis and played an important role. The results presented in this study reveal the physicochemical properties and expression patterns of ACA/CA family members for the first time in rubber tree, and would provide a foundation for further investigation of the biological functions of ACA/ECA genes in H. brasiliensis, especially in the regulation of the rubber production.

Key words: Hevea brasiliensis, Ca2+-ATPase (ACA/ECA), gene family, biological functiun, expression analysis

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