Rapid Multiplication and <i>in vitro</i> Flowering of <i>Mengzia foliosa</i> (King & Pantl.) W.C. Huang, Z.J. Liu & C. Hu (Orchidaceae)
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2236-2243.DOI: 10.3969/j.issn.1000-2561.2023.11.012

• Special Topic of Orchid Science • Previous Articles     Next Articles

Rapid Multiplication and in vitro Flowering of Mengzia foliosa (King & Pantl.) W.C. Huang, Z.J. Liu & C. Hu (Orchidaceae)

SHAO Li1, ZENG Xinhua1, HUANG Weichang1,2,*()   

  1. 1. Shanghai Chenshan Botanical Garden / Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai 201602, China
    2. College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2023-06-28 Revised:2023-07-25 Online:2023-11-25 Published:2023-12-08
  • Contact: *HUANG Weichang,E-mail:huangweichang@csnbgsh.cn

Abstract:

In this study, the effects of different concentration and ratio of growth regulators on seed germination, rapid propagation and in vitro flowering of Mengzia foliosa were studied. The results showed that the seeds had strong activity and high germination rate at 105 days after artificial self-pollination, and 1/2MS+0.6 mg/L NAA+1.0 mg/L 6-BA medium was more suitable for seed germination. Meanwhile, 2.0 mL/L 6-BA without NAA was more suitable for cluster bud proliferation; and 1/2MS+1.0 mL/L NAA+30 g/L potato homogenate medium was the best for rooting and hardening-off. In addition, 1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L PP333 medium had the highest flowering rate. Our results not only contribute to the conservation and sustainable use of the species, but also provide important materials and technical support for wild population restoration and reconstruction.

Key words: Mengzia foliosa, aseptic sowing, rapid propagation, in vitro flowering

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