Genetic Diversity of Wild <i>Paphiopedilum hirsutissimum </i>Populations in Southwest China with SSR Markers
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2208-2218.DOI: 10.3969/j.issn.1000-2561.2023.11.009

• Special Topic of Orchid Science • Previous Articles     Next Articles

Genetic Diversity of Wild Paphiopedilum hirsutissimum Populations in Southwest China with SSR Markers

XU Yan1,2, CHEN Zhiguang2, XU Yufeng2, GE Hong2, YANG Shuhua2, ZHAO Xin2, KOU Yaping2, YU Xiaonan1,*(), JIA Ruidong2,*()   

  1. 1. College of Landscape Architecture, Beijing Forestry University / Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding / National Engineering Research Center for Floriculture / Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 100083, China
    2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Flower Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2022-12-20 Revised:2023-01-31 Online:2023-11-25 Published:2023-12-08
  • Contact: *YU Xiaonan,E-mail:yuxiaonan626@126.com;JIA Ruidong,E-mail:jiaruidong@caas.cn

Abstract:

Paphiopedilum hirsutissimum (Lindl. ex Hook. f.) Stein is a rare and endangered plant in China with high ornamental value. In order to explore the genetic characteristics of wild P. hirsutissimum resources in Southwest China, and contribute to the protection and utilization of the wild resources, this study used SSR molecular marker to analyze the genetic diversity and population genetic structure of 190 P. hirsutissimum resources which collected from six populations in Guangxi, Yunnan and Guizhou provinces in Southwest China. In this study, the results showed that ten pairs of primers with good amplification effect were selected from 115 pairs of primers, and 50 alleles were detected by 10 pairs of SSR primers of 190 P. hirsutissimum. The average number of alleles (Na) was five and the average number of effective alleles (Ne) was 2.4835. The average Shannon information index (I) was 0.8592. The average observed heterozygosity (Ho) was 0.4518 and the average expected heterozygosity (He) was 0.4387. The average polymorphic information content (PIC) was 0.3996 and the average Nei’s expected heterozygosty (h) was 0.4370. In this six wild P. hirsutissimum populations, the number of alleles (Na) was from 2.8000 to 4.3000. The number of effective alleles (Ne) was from 1.9655 to 2.4060. The observed heterozygosity (Ho) was from 0.3891 to 0.4839. The expected heterozygosity (He) was from 0.3795 to 0.4683. The Shannon information index (I) was from 0.6584 to 0.8369, and the Nei’s expected heterozygosty (h) was from 0.3648 to 0.4382. In the six wild populations, the genetic diversity of Guangxi Yachang (GYC) and Guizhou Wanfeng Lake (QWF) populations was generally higher (h=0.4382, h=0.4276), while the genetic diversity of Guangxi Mulun (GML) population was relatively low (h=0.3648). Analysis of molecular variance (AMOVA) results showed that genetic variation mainly occurred among individuals within the population (94%), while genetic differentiation within populations was very small (6%). UPGMA cluster analysis based on genetic distance showed that the genetic distance of the six P. hirsutissimum populations was very small, there was no obvious group division and the genetic distance was not completely related to the geographical location. The results of Structure and principal coordinate analysis were consistent with those of UPGMA cluster analysis. Structure and principal coordinate analysis results showed that there was homogenization among the populations, and there was no obvious group division. In summary, the genetic diversity of Paphiopedilum hirsutissimum resources is relatively rich, which can provide a theoretical basis for the protection and utilization of wild P. hirsutissimum resources in Southwest China.

Key words: Paphiopedilum hirsutissimum, wild populations, SSR, genetic diversity, genetic structure

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