Metabolomics Analysis of Anthocyanin in Different Flower Colors of <i>Phalaenopsis-</i>Type<i> Dendrobium</i>
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (11): 2167-2178.DOI: 10.3969/j.issn.1000-2561.2023.11.004

• Special Topic of Orchid Science • Previous Articles     Next Articles

Metabolomics Analysis of Anthocyanin in Different Flower Colors of Phalaenopsis-Type Dendrobium

WU Meiqing1,2, LIAO Yi2,3, LU Shunjiao2,3, YIN Hantai1,2, YU Wengang1,*(), LI Chonghui2,3,*()   

  1. 1. College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Tropical Crop Germplasm Research Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, Hainan 571101, China
    3. Hainan Provincial Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, Hainan 571737, China
  • Received:2023-07-26 Revised:2023-09-12 Online:2023-11-25 Published:2023-12-08
  • Contact: *LI Chonghui,E-mail:blchh@sina.com;YU Wengang,E-mail:yuwg2008@hainanu.edu.cn

Abstract:

In order to further investigate the anthocyanin biosynthetic pathway in Phalaenopsis-type Dendrobium (Den-Phals) varieties with different flower color, this study used widely targeted metabolomics technology to analyze the anthocyanin composition and content of five Den-Phals varieties with blue-purple, light peach-red, red, purple-red and deep purple-red floral color, respectively. The results showed that a total of 38 metabolites were identified from the tested Den-Phals, most of which were glycosides and acylated derivatives of cyanidin (Cy), peonidin (Pn), pelargonidin (Pg), delphinidin (Dp) and petunidin (Pt). There were significant differences in the composition and content of anthocyanin in five Den-Phals varieties. Metabolite difference analysis showed that Cy and Dp glycosides in purple flowers were significantly higher than those in light peach red flowers. The contents of three Dp-type glycosides in blue-purple flowers were significantly higher than those in other colored varieties. Only one Cy-type glycoside was screened out, and the content of which in light pink flowers was high. The content of Cy-type glycosides was the highest in most samples. With the increase of cyanidin and delphinidin and the derivatives, the color of flowers deepened and turned to red-purple. It was speculated that Cy-type glycosides made Den-Phals tend to purple while Dp glycosides gave Den-Phals a blue-purple tone. Based on the above results, it is speculated that there are acylation synthesis pathways of the above five anthocyanins in Den-Phals, which can provide a basis for flower color formation mechanism and flower color improvement of Den-Phals.

Key words: Phalaenopsis-type Dendrobium, flower color, metabolome, anthocyanins

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