Morphological Characteristics and the Change Trends of the Internal Reproductive System of Paurocephala sauteri Adults in Different Developmental Stage

doi:10.3969/j.issn.1000-2561.2023.05.017

Abstract

Abstract:

Paurocephala sauteri, characterized by small individuals, rapid reproduction and overlapped generations, is the most damaging pest of mulberry trees in tropical and subtropical areas. It happens in seedling period, leading to yield loss over 40%, or even out of harvest, and is very difficult to control. In order to accurately predict and efficiently control the pest, and to improve the yield and quality of mulberry leaves, the internal reproductive system of the male and female adults of the pest in different developmental periods was dissected and observed to clarify the morphological characteristics and changes of the internal reproductive system of male and female adults of the pest before and after mating. Except for 2 pairs of lateral oviducts the composition of the internal reproductive system of the female adults is similar to Trioza erytreae (Del guercio), and male adults is highly similar to Diaphorina citri (Kuwayama). Compared with the pre-mating period, the length of spermatheca in mating and post-mating periods reached nearly 1.63 times and 1.85 times, and the width increased by about 1.74 times and 1.84 times respectively, with significant differences in each period (P<0.05), the length and width of eggs increased rapidly, to mating period increased by nearly 2 times (P<0.05). At the same time, several large vesicles appeared inside the egg in mating period and disappeared in post-mating period, them should be trophoblasts, providing nutrition for oocytes. Compared with the pre-mating period, the testes of the male increased to the maximum in the mating period and shrank to the minimum in the post-mating period. There were significant differences in the three periods (P<0.05), while the length and width of the seminal vesicle both significantly increased to about twice (P<0.05) in mating and post-mating periods. This study has practical significance to precisely judge and determine the appropriate period of prevention and control, to clone reproductive-related genes, to develop new gene targeting agents, and to formulate efficient prevention and control measures of P. sauteri.

Key words: Paurocephala sauteri, internal reproductive system, morphological characteristics, change trends

CLC Number:

S888.723

WEI Hongxian, GENG Tao, WU Huazhou, LOU Dezhao, WU Chunbiao, XIE Yunhui, LU Fuping, WANG Shuchang. Morphological Characteristics and the Change Trends of the Internal Reproductive System of Paurocephala sauteri Adults in Different Developmental Stage[J]. Chinese Journal of Tropical Crops, 2023, 44(5): 1023-1029.

Figures/Tables 6

Fig. 1 Internal reproductive system of adult female P. sauteri

Fig. 2 Morphological characteristics of internal reproductive system of adult male P. sauteri

Tab. 1 Size change of spermatheca and egg of female adult P. sauteri in different developmental periods μm

时期 Period	受精囊Spermatheca		卵Egg
时期 Period	长度Length	宽度Width	长度Length	宽度Width
交配前期	897.57±48.23^c	505.58±23.42^c	216.95±26.64^c	112.09±17.41^b
交配期	1463.54±30.72^b	878.13±25.30^b	562.50±21.66^b	283.59±22.12^a
交配后期	1662.11±55.36^a	932.29±56.33^a	617.68±22.61^b	281.47±20.35^a

Fig. 3 Morphological characteristics of internal reproductive system of female adult P. sauteri in different periods A: Pre-mating period; B: Mating period; C: Post-mating period. Side light source photos of didital biomicros-copy AT3116 are on the left and bottom light source photos are on the right.

Tab. 2 Size change of testis and seminal vesicle of male adult P. sauteri in different developmental periods μm

时期 Period	精巢Testis		贮精囊Seminal vesicle
时期 Period	长度Length	宽度Width	长度Length	宽度Width
交配前期	1200.89±21.02^b	497.40±13.04^b	343.75±31.25^c	329.69±28.95^b
交配期	1342.19±37.16^a	546.88±28.53^a	809.38±37.30^a	635.42±36.53^a
交配后期	1009.94±64.15^c	487.98±24.00^b	760.94±27.61^b	623.96±49.93^a

Fig. 4 Morphological characteristics of internal reproductive system of male adult P. sauteri in the different periods A: Pre-mating period; B: Mating period; C: Post-mating period. Side light source photos of didital biomicros-copy AT3116 are on the left and bottom light source photos are on the right.

References 23

[1]	卢芙萍, 耿涛, 武华周, 王娜玉, 汪永松, 娄德钊, 王树昌. 桑小头木虱Paurocephala sauteri Enderlein生物学特性研究[J]. 热带作物学报, 2020, 41(9): 1883-1888.
	LU F P, GENG T, WU H Z, WANG N Y, WANG Y S, LOU D Z, WANG S C. Biological characteristics of muiberry psyllid Paurocephala sauteri Enderlein (Hemiptera: Psylloidea: Psyllidae)[J]. China Journal of Tropical Crops, 2020, 41(9): 1883-1888. (in Chinese)
[2]	李法圣. 中国木虱志[M]. 北京: 科学出版社, 2011: 226-227.
	LI F S. Psyllidomorpha of China[M]. Beijing: Science Press, 2011: 226-227. (in Chinese)
[3]	YI C L, MAN M Y. First record of the mulberry psyllid Anomoneura mori Schwarz (Hemiptera: Psylloidea: Psyllidae) from Taiwan[J]. Journal of Asia-Pacific Entomology, 2018(2): 603-608.
[4]	杨集昆, 李法圣. 小头木虱属五新种及母生滑头木虱新属种(同翅目: 木虱科: 小头木虱亚科)[J]. 武夷科学, 1986, 6: 45-58.
	YANG J K, LI F S. Six new species and a new genus of Paurocephalinae (Psyllidae: Homoptera)[J]. Wuyi Science Journal, 1986, 6: 45-58. (in Chinese)
[5]	唐毅. 西南地区木虱总科区系分类研究(半翅目: 胸喙亚目)[D]. 贵阳: 贵州大学, 2007.
	TANG Y. Taxonomic and faunistic study on Psyllozdea (Hemiptera) from SW-China[D]. Guiyang: Guizhou University, 2007. (in Chinese)
[6]	MIFSUD D, BURCKHARDT D. Taxonomy and phylogeny of the Old World jumping plant-louse genus Paurocephala (Insecta, Hemiptera, Psylloidea)[J]. Journal of Natural History, 2002, 36: 1887-1986. DOI URL
[7]	CRAWFORD D L. The Psyllidae of borneo[J]. The Philippine Journal of Science, 1920, 17(4): 353-359. DOI URL
[8]	KUMUYAMA S. A revision of the Psyllidae of Taiwan[ J]. Insecta Matsumurana, 1931, 5(3): 117-133.
[9]	肖培彬, 马义雷, 李皓, 韩群鑫. 亚洲柑橘木虱内生殖系统形态变化规律研究[J]. 环境昆虫学报, 2017, 39(6): 1207-1213.
	XIAO P B, MA Y L, LI H, HAN Q X. Morphological changes of internal reproductive system of Diaphorina citri Kuwayama[J]. Journal of Environmental Entomology, 2017, 39(6): 1207-1213. (in Chinese)
[10]	李文敬, 李新畅, 李旋, 崔娟, 徐伟, 张金平, 史树森. 大豆食心虫成虫性比对寿命和生殖力的影响[J]. 大豆科学, 2020, 39(3): 451-457.
	LI W J, LI X C, LI X, CUI J, XU W, ZHANG J P, SHI S S. Effect of sex ratio on fecundity and lifetime of Leguminivora glycinivorelle adults[J]. Soybean Science, 2020, 39(3): 451-457. (in Chinese)
[11]	李俊. 桃小食心虫和梨小食心虫生殖及消化系统的组织细胞学研究[D]. 太原: 山西农业大学, 2016.
	LI J. Histocytology study of the reproductive and digestive systems of Carposina sasakii and Grapholita molesta[D]. Taiyang: Shanxi Agricultural University, 2016. (in Chinese)
[12]	张万娜, 肖海军, 梁革梅, 郭予元. 棉铃虫卵巢形态与卵子发生过程观察[J]. 昆虫学报, 2013, 56(4): 358-364.
	ZHANG W N, XIAO H J, LIANG G M, GUO Y Y. Observation on ovarian morphology and oogenesis in cotton bollworm[J]. Acta Entomologica Sinica, 2013, 56(4): 358-364. (in Chinese)
[13]	夏亚运. 红颈常室茧蜂卵巢发育及其生殖相关蛋白研究[D]. 海口: 海南大学, 2017.
	XIA Y Y. The ovarian development and reproduction-related proteins in Peristenus spretus Chen et van Achterbery[D]. Haikou: Hainan University, 2017. (in Chinese)
[14]	文靓, 陈丰, 陈亚青, 周来娣, 刘玉成, 冯启理, 刘琳. 斜纹夜蛾精巢的发育和精子的发生[J]. 华南师范大学学报(自然科学版), 2019, 51(4): 47-56.
	WEN L, CHEN F, CHEN Y Q, ZHOU L D, LIU Y C, FENG Q L, LIU L. The testis development and spermatogenesis in Spodoptera litura (Lepidoptera: Noctuidae)[J]. Journal of South China Normal University (Natural Science Edition), 2019, 51(4): 47-56. (in Chinese)
[15]	罗静. 中黑盲蝽信息素合成相关基因的筛选及功能分析[D]. 武汉: 华中农业大学, 2017.
	LUO J. Screening and function analysis of pheromone biosynthesis relateg-genes in Adelphocoris suturalis[D]. Wuhan: Huazhong Agricultural University, 2017. (in Chinese)
[16]	雷晨. 白背飞虱、灰飞虱Doublesex基因分析[D]. 杭州: 浙江大学, 2018.
	LEI C. Functional analysis of Doublesex gene in Sogatella furcifera and Laodelphax striatellus[D]. Hangzhou: Zhejiang University, 2018. (in Chinese)
[17]	LI D T, DAI Y T, CHEN X, WANG X Q, LI Z D, MOUSSIAN B, ZHANG C X. Ten fatty acyl-CoA reductase family genes were essential for the survival of the destructive rice pest, Nilaparvata lugens[J]. Pest Management Science, 2020, 76(7): 2304-2315. DOI URL
[18]	夏婷. 褐飞虱雄虫生殖基因NlSPATA5和NlPHF7调控雌虫生殖及种群增长的研究[D]. 扬州: 扬州大学, 2017.
	XIA T. The regulatory roles of two male-derived genes in female fecundity and population growth in brown planthopper Nilaparvata lugens[D]. Yangzhou: Yangzhou University, 2017. (in Chinese)
[19]	BLOWERS J R, MORAN V G. Notes on the female reproductive system of the South African citrus psylla, Trioza erytreae (Del Guercio) (Homptera: Psyllidae)[J]. Journal of the Entomological Society of Southern Africa, 1967, 30(1): 75-81.
[20]	张晓红, 李敏, 张虎芳. 缘蝽总科4种雄性昆虫减数分裂期染色体形态分析(半翅目: 缘蝽总科)[J]. 西北农林科技大学学报(自然科学版), 2013, 41(4): 169-177.
	ZHANG X H, LI M, ZHANG H F. Morphology of chromosome during meioticof 4 male insects Coreoidea (Hemiptera: Coreoidea)[J]. Journal of Northwest A & F University (Natural Science Edition), 2013, 41(4): 169-177. (in Chinese)
[21]	SOUZA H V, ARAKAKI R L M, DIAS L N, MURAKAMI A S, COSTA L A A, BICUDO H E M DE C, ITOYAMA M M. Cytogenetical aspects of testicular cells in economically important species of Coreidae family (Heteroptera)[J]. Cytologia, 2007, 72: 49-56. DOI URL
[22]	SOUZA H V, SOUZA F B, MARUYAMA S R C, CASTANHOLE M M U, ITOYAMA M M. Meiosis, spermatogenesis and nucleolar behavior in the seminiferous tubules of Alydidae, Coreidae and Rhopalidae (Hetewptema) species[J]. Genetics and Molecular Research, 2009, 8(4): 1383-396. DOI URL
[23]	卢芙萍, 耿涛, 武华周, 娄德钊, 涂娜娜, 王树昌. 桑小头木虱对不同颜色的趋性[J]. 果树学报, 2021, 38(12): 2156-2165.
	LU F P, GENG T, WU H Z, LOU D Z, TU N N, WANG S C. Evaluation on color preference of adult mulberry psyllid (Paurocephala sauteri Enderlein)[J]. Journal of Fruit Science, 2021, 38(12 )