Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2023, Vol. 44 ›› Issue (5): 977-985.DOI: 10.3969/j.issn.1000-2561.2023.05.013

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Establishment of Callus Regeneration System of Hippeastrum ‘Bangkok Rose’

YANG Wei1,2, LIU Xinyi1,2, ZENG Jingjue2, WU Kuilin2, FANG Lin2, WU Shasha1, ZHAI Junwen1,*(), ZENG Songjun1,2,3,*()   

  1. 1. College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China
    3. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China
  • Received:2022-10-18 Revised:2022-11-08 Online:2023-05-25 Published:2023-06-07
  • Contact: *ZHAI Junwen,E-mail:zhai-jw@163.com;ZENG Songjun,E-mail:zengsongjun@scib.ac.cn


Hippeastrum is a new kind of flower introduced in large scale from abroad in recent years, but its seedlings are expensive, regular scale cuttage propagation speed is slow and needs a large number of mother bulbs. Cross breeding, a common method for its new varieties, however, has poor orientation. This aim of this study was to establish an efficient callus regeneration system for rapid propagation and factory production of seedlings, which can also be used for the orientation breeding of Hippeastrum. The effects about different plant growth regulators on callus induction and plantlet regeneration were studied with the leaves of Hippeastrum ‘Bankkok Rose’ plantlet in vitro. The highest induction rate of callus (39.67%) was observed when the basal leaves of the buds in vitro were cultured on MS+2,4-D 2.00 mg/L+ TDZ 0.50 mg/L medium for 45 days. The optimal medium for callus proliferation was MS+6-BA 2.00 mg/L, and the average proliferation was 4.01 times every 20 days. The optimal medium for callus differentiation was MS+ KT 0.50 mg/L. After 60 days, the adventitious bud differentiation coefficient reached 10.59, and the seedling formation coefficient was 5.67. The rooting rate reached 100% after 30 days in MS+IBA 0.50 mg/L. After 30 days of root culture, the small plants were transplanted to the coir: peat soil: vermiculite =1 : 1 : 1 substrate, and the survival rate reached 93.33% after 30 days. This study could provide technical support for industrial propagation of Hippeastrum seedlings, and also provide excellent receptor materials for subsequent molecular breeding.

Key words: Hippeastrum, leave in vitro, plant growth regulators, tissue culture

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